Team:Paris Saclay/Notebook/June/30

From 2014.igem.org

(Difference between revisions)
(2 - Réhydration of selected biobricks:)
(2 - Réhydration of selected biobricks:)
Line 16: Line 16:
''by Juliette''
''by Juliette''
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* BBa_I9026
+
* BBa_I9026 (rhII translational unit)
-
* BBa_K592025
+
* BBa_K592025 (BB0034-amilCP)
-
* BBa_K786002
+
* BBa_K786002 (sensory rhodopsin II & Tar sensitive to blue-green light)
-
* BBa_K786003
+
* BBa_K786003 (sensory rhodopsin I & Tar sensitive to orange light)
-
* BBa_K778002
+
* BBa_K778002  
-
* BBa_K592011 {2 times}
+
* BBa_K592011 (cjBlue green chromoprotein)(2 times)
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* BBa_K592009
+
* BBa_K592009 (amilCP blue chromoprotein)
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* BBa_K1033910 {idem}
+
* BBa_K103391 (Yellow chromoprotein and Lime yellow-green chromoprotein)(2 times)
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* BBa_J45014
+
* BBa_J45014 (lemon scent, alcohol acetyltransferase I)
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* BBa_K517003
+
* BBa_K517003 (lemon scent, β-pinene)
====3 - Transformations :====
====3 - Transformations :====

Revision as of 08:02, 25 July 2014

Contents

Monday 30th June

Lab work

1 - Culture or the supercompetents:

by Mathias

  • MG1655
  • MG1655Z1

1ml of an overnight culture in 100ml of LB.

2 - Réhydration of selected biobricks:

by Juliette

  • BBa_I9026 (rhII translational unit)
  • BBa_K592025 (BB0034-amilCP)
  • BBa_K786002 (sensory rhodopsin II & Tar sensitive to blue-green light)
  • BBa_K786003 (sensory rhodopsin I & Tar sensitive to orange light)
  • BBa_K778002
  • BBa_K592011 (cjBlue green chromoprotein)(2 times)
  • BBa_K592009 (amilCP blue chromoprotein)
  • BBa_K103391 (Yellow chromoprotein and Lime yellow-green chromoprotein)(2 times)
  • BBa_J45014 (lemon scent, alcohol acetyltransferase I)
  • BBa_K517003 (lemon scent, β-pinene)

3 - Transformations :

by Maher & Mathias

We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10-1 and 10-2.

4 - Preparations :

  • Aliquots of antibiotics :

by Marie & Mathieu

Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)

  • Petri dishes

by Romain

Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.

  • Range of gelose with different concentration of agar

by Arnaud

Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.

Members present:

  • Instructors : Solenne.
  • Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

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