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Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP
From 2014.igem.org
(Difference between revisions)
| Line 27: | Line 27: | ||
Attempt 1: | Attempt 1: | ||
<ul> | <ul> | ||
| - | <li>Start date/time:</li> | + | <li>Start date/time: 24/07, 11:08</li> |
| - | <li>End date/time:</li> | + | <li>End date/time:24/07, 1550</li> |
<li> UIDs of primers/plasmids used: | <li> UIDs of primers/plasmids used: | ||
<ul> | <ul> | ||
| - | <li> | + | <li> A1, P33, P34</li> |
| - | <li> | + | <li> A1, P35, P36</li> |
| - | <li> | + | <li> A2, P37, P38</li> |
| + | <li> A2, P39, P40 </ul></li> | ||
<li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li> | <li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li> | ||
Revision as of 14:30, 24 July 2014
Gal4 - Hap1GR - H2BmRFP
Design
Aim of the construct
- Describe aims here
End goal plasmid
Experimentation (up to date)
PCR
Attempt 1:- Start date/time: 24/07, 11:08
- End date/time:24/07, 1550
- UIDs of primers/plasmids used:
- A1, P33, P34
- A1, P35, P36
- A2, P37, P38
- A2, P39, P40
- PCR Spreadsheet (on google drive)
- Gel 27/07/2014
Gibson
Attempt 1:- Start date/time:
- End date/time:
- Comments:
E-Coli transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Agrobacteria transformation
Attempt 1:- Start date/time:
- End date/time:
- Induce comments:
- Growth comments:
- Electroporation/selection comments:
Spore preparation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Spore transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Evaluation
- Start date/time:
- End date/time:
- Comments:
