Team:Paris Saclay/Notebook/July/24

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(→Thursday 24th July)
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We have to make another PCR.
We have to make another PCR.
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===E - The Suppressing Salicylate System===
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====Transformation of competent E.coli cells====
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''by Fabio''
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We transformed in DH5α both BioBricks rehydrated [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23 yesterday] ('''BBa_J61051''' and '''BBa_K228001''').
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'''Samples:'''
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# BBa_J61051 Concentrated
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# BBa_J61051 Undiluted
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# BBa_K228001 Concentrated
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# BBa_K228001 Undiluted
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# DH5α Control
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
'''Members present:'''
'''Members present:'''

Revision as of 13:05, 24 July 2014

Contents

Thursday 24th July

Lab work

C - Lemon scent

Results of the PCR Targeting

by Romain

PCR targeting made the July 22nd.

Results:

  • Nothing has grown on the control dishes.
  • Failure, nothing has grown on the clone I dishes.
  • Failure, other bacteria have grown on the clone II dishes.

We have to make another PCR.

E - The Suppressing Salicylate System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α both BioBricks rehydrated yesterday (BBa_J61051 and BBa_K228001).

Samples:

  1. BBa_J61051 Concentrated
  2. BBa_J61051 Undiluted
  3. BBa_K228001 Concentrated
  4. BBa_K228001 Undiluted
  5. DH5α Control

Protocol

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/24"