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Team:Paris Saclay/Notebook/July/23
From 2014.igem.org
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==Lab work== | ==Lab work== | ||
| - | ===1 - p cola part 2=== | + | ====1 - p cola part 2==== |
''by Sean'' | ''by Sean'' | ||
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# Discard supernatant. Make tube interior as dry as possible. '''Tip''': use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. <span style="color:red">Note: we decided to leave the tube at room temperature and continue tomorrow instead.</span> | # Discard supernatant. Make tube interior as dry as possible. '''Tip''': use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. <span style="color:red">Note: we decided to leave the tube at room temperature and continue tomorrow instead.</span> | ||
# <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font> | # <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font> | ||
| + | |||
| + | ===E - The Suppressing Salicylate System=== | ||
| + | |||
| + | ====Rehydration of BioBricks==== | ||
| + | ''by Fabio'' | ||
| + | |||
| + | The first step of this Subproject is to rehydrate the following BioBricks. Both provided in the iGEM 2014 DNA Kit Distribution. | ||
| + | |||
| + | * BBa_J61051 - ''Salicylate promoter + NahR'' | ||
| + | * BBa_K228001 - ''RNA suppressor'' | ||
==Reunion== | ==Reunion== | ||
| - | + | Handled topics: | |
| + | * Collaborations with Colombia, Paris Bettencourt and Virginia | ||
| + | * Organisation of the Meeting with french teams | ||
| + | * Definitions of the sub projects | ||
| + | * Definitions of notebook's standards | ||
| + | * Organisation of the Wiki | ||
| + | * Great discussion about Human Practices | ||
'''Members there''': | '''Members there''': | ||
Revision as of 12:56, 24 July 2014
Contents |
Wednesday 23rd July
Lab work
1 - p cola part 2
by Sean
p cola DNA was extracted in several instances yesterday. Today we attempt to collect all of the DNA and precipitate it.
Protocol
- Add equal parts plasmid DNA and ethanol 100% in a 1.5 microcentrifuge tube.
- If total volume is V, then add V/5 of sodium acetate (initial concentration of NaAc is 3M; we want a final concentration of .3M). Numerical application: Total volume of p cola was 150μl. Hence 150 μl of ethanol 100% was added, as well as 30μl of NaAc 3M. Incubate at -20°C for 30 minutes. (5:20pm - 5:50pm in our case)
- Centrifuge for 10 minutes at 4°C and at 11000g.
- Discard supernatant.
- Add 1ml of ethanol 70%.
- Repeat step 3.
- Discard supernatant. Make tube interior as dry as possible. Tip: use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. Note: we decided to leave the tube at room temperature and continue tomorrow instead.
- (tomorrow) Resuspend pellet in 30μl H2O milliQ.
E - The Suppressing Salicylate System
Rehydration of BioBricks
by Fabio
The first step of this Subproject is to rehydrate the following BioBricks. Both provided in the iGEM 2014 DNA Kit Distribution.
- BBa_J61051 - Salicylate promoter + NahR
- BBa_K228001 - RNA suppressor
Reunion
Handled topics:
- Collaborations with Colombia, Paris Bettencourt and Virginia
- Organisation of the Meeting with french teams
- Definitions of the sub projects
- Definitions of notebook's standards
- Organisation of the Wiki
- Great discussion about Human Practices
Members there:
- Instructors and advisors: Solenne and Sylvie.
- Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.
