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Team:BYU Provo/Notebook/Metabolism/julyaug
From 2014.igem.org
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| + | <h2>Week of July 5th</h2> | ||
| + | <h3>June 30, 2014</h3> | ||
| + | <p>--BRK--I chose one colony of E.coli from four separate LB and CAM plates containing transformed bacteria with EreB and a promoter of either 101, 108, 111, or 119. These four colonies were streaked on four separate LB, CAM, and Erythromycin plates and grown overnight at 37C.</p> | ||
| + | <h3>July 1, 2014</h3> | ||
| + | <p>--BRK--The streaked colonies grew well on the LB/CAM/Erythromycin plates, indicating that the plasmid with the EreB gene had been successfully transformed. I took pictures of all the plates to compare the growth and use in the future to determine the effect of the different promoters.</p> | ||
| + | <h3>July 3, 2014</h3> | ||
| + | <p>--BRK--Our team went to the wastewater treatment plant to learn more about the entire treatment process. I collected the biofilm and effluent water run-off from the plant to bring back to the lab for testing.</p> | ||
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| + | <h2>Week of July 12th</h2> | ||
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| + | <h2>Week of July 19th</h2> | ||
| + | <h2>Week of July 26th</h2> | ||
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Revision as of 22:25, 23 July 2014
BYU 2014 Notebook |
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Week of July 5th
June 30, 2014
--BRK--I chose one colony of E.coli from four separate LB and CAM plates containing transformed bacteria with EreB and a promoter of either 101, 108, 111, or 119. These four colonies were streaked on four separate LB, CAM, and Erythromycin plates and grown overnight at 37C.
July 1, 2014
--BRK--The streaked colonies grew well on the LB/CAM/Erythromycin plates, indicating that the plasmid with the EreB gene had been successfully transformed. I took pictures of all the plates to compare the growth and use in the future to determine the effect of the different promoters.
July 3, 2014
--BRK--Our team went to the wastewater treatment plant to learn more about the entire treatment process. I collected the biofilm and effluent water run-off from the plant to bring back to the lab for testing.
