Team:Paris Saclay/Notebook/July/22

From 2014.igem.org

(Difference between revisions)
(→C - Lemon scent)
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''by Romain''
''by Romain''
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Strains used: DY330  
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Strains used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)
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Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)
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1- Dilution of 300µl of bacterial culture in 30ml of LB + 30µl Cm at 30°C.
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2- With the pre-culture:
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*make glycerol stock: 1ml bacterial culture with 240µl glycerol 87%.
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*Make extraction of 5ml of plasmid [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
'''Members there''':
'''Members there''':

Revision as of 20:18, 23 July 2014

Contents

Tuesday 22st July

Lab Work

1 - Samples for stock

by Fabio

We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C.

  • BBa_K1033902
  • BBa_K1033905
  • BBa_K1033910
  • BBa_K1033913
  • BBa_K1033922
  • BBa_K1033925
  • BBa_K1033927
  • BBa_K1033929
  • BBa_K103921
  • BBa_J45017
  • BBa_K731201
  • BBa_K762100

from Liquide Bacterial Cultures transformed the 21st July

2 - Electrophoresis

by Arnaud (Process B), Fabio (gel), Marie and Romain (Process B)

We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel.

Process A

Process B

Results:

  • A:
  • B:
  • C:

Protocol

3 - Bacterial Culture

by Fabio and Marie

One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony.

4 - Plasmid DNA extraction

by Sean and Terry

Plasmids used: cf part 1

Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.

C - Lemon scent

PCR Targeting

by Romain

Strains used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)

1- Dilution of 300µl of bacterial culture in 30ml of LB + 30µl Cm at 30°C.

2- With the pre-culture:

  • make glycerol stock: 1ml bacterial culture with 240µl glycerol 87%.
  • Make extraction of 5ml of plasmid Protocol

Members there:

  • Instructors and advisors: Solenne.
  • Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.

Back to the calendar

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