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Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
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| - | * | + | * BBa_K773002 (x2) |
| - | * | + | * BBa_K517003 (x2) |
| - | * | + | * BBa_K773002 (x2) |
| - | * | + | * BBa_K592011 (x2) |
| - | * | + | * BBa_K1033910 (x2) |
| - | * | + | * BBa_K592009 (x2) |
| - | * | + | * BBa_K786002 (x2) |
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| - | * | + | * BBa_J45014 (x2) |
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | ||
Revision as of 15:36, 23 July 2014
Contents |
Thursday 3rd July
Lab Work
1 - Phages P1 transduction in MG1655 and MG1655Z1
by Mathias
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation
Pour in LB Kan petri dishes, incubate at 37°C. (Turn the petri dishes over when there is no more liquid in!)
2 - Plasmid DNA Purification
by Fabio, Maher, Mathieu, Marie and Romain
- BBa_K773002 (x2)
- BBa_K517003 (x2)
- BBa_K773002 (x2)
- BBa_K592011 (x2)
- BBa_K1033910 (x2)
- BBa_K592009 (x2)
- BBa_K786002 (x2)
- BBa_K786003 (x2)
- BBa_J45014 (x2)
- BBa_I9026 (x2)
Members present:
- Instructors : Alice & Solenne.
- Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
