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Team:Paris Saclay/Notebook/July/22
From 2014.igem.org
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We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C. | We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C. | ||
| - | * | + | *BBa_K1033902 |
| - | * | + | *BBa_K1033905 |
| - | * | + | *BBa_K1033910 |
| - | * | + | *BBa_K1033913 |
| - | * | + | *BBa_K1033922 |
| - | * | + | *BBa_K1033925 |
| - | * | + | *BBa_K1033927 |
| - | * | + | *BBa_K1033929 |
| - | * | + | *BBa_K103921 |
| - | * | + | *BBa_J45017 |
| - | * | + | *BBa_K731201 |
| - | * | + | *BBa_K762100 |
from Liquide Bacterial Cultures transformed the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July] | from Liquide Bacterial Cultures transformed the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July] | ||
Revision as of 14:37, 23 July 2014
Contents |
Tuesday 22st July
Lab Work
1 - Samples for stock
by Fabio
We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C.
- BBa_K1033902
- BBa_K1033905
- BBa_K1033910
- BBa_K1033913
- BBa_K1033922
- BBa_K1033925
- BBa_K1033927
- BBa_K1033929
- BBa_K103921
- BBa_J45017
- BBa_K731201
- BBa_K762100
from Liquide Bacterial Cultures transformed the 21st July
2 - Electrophoresis
by Arnaud (Process A), Fabio (gel), Marie and Romain (Process B)
We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel.
Process A
Process B
Results:
- A:
- B:
- C:
3 - Bacterial Culture
by Fabio and Marie
One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony.
4 - Plasmid DNA extraction
by Sean and Terry
Plasmids used: cf part 1
Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.
Members there:
- Instructors and advisors: Solenne.
- Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
