Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP

From 2014.igem.org

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(Created page with "<div id="constructs" align="center"> Constructs: <ul> <li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/gal4_hap1gr_H2RBmRFP">Full circuit attempt [SSB]</a></li> </...")
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<div id="constructs" align="center">
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<html>
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Constructs:
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<h1> Gal4 - Hap1GR - H2BmRFP>
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<h2> Design </h2>
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<div id="Aim" align="left">
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<h4> Aim of the construct </h4>
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<ul>
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<li> Describe aims here </li>
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</ul>
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</div>
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<div id="The plasmid" align="left">
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<h4> End goal plasmid </h4>
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<ul>
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<li> insert image here </li>
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</ul>
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</div>
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<h2> Experimentation (up to date)</h2>
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<div id="PCR" align="left">
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<h4>PCR</h4>
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Attempt 1:
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<ul>
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<li>Start date/time:</li>
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<li>End date/time:</li>
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<li> UIDs of primers/plasmids used:
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<li> A2, P12, P13</li>
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<li> B3, P14, P13</li>
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<li> A4, P12, P13 </li>
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<li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li>
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<li><a href="http://google.com/GEL_27_07_2014">Gel 27/07/2014</a></li>
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</ul>
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</div>
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<div id="Gibson" align="left">
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<h4> Gibson </h4>
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Attempt 1:
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<ul>
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<li>Start date/time:</li>
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<li>End date/time:</li>
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<li>Comments:</li>
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</ul>
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</div>
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<div id="E-Coli transformation" align="left">
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<h4>E-Coli transformation </h4>
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Attempt 1:
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<ul>
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<li>Start date/time:</li>
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<li>End date/time:</li>
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<li>Comments:</li>
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</ul>
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</div>
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<div id="Agrobacteria transformation" align="left">
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<h4>Agrobacteria transformation</h4>
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Attempt 1:
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<ul>
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<li>Start date/time:</li>
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<li>End date/time:</li>
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<li>Induce comments:</li>
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<li>Growth comments:</li>
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<li>Electroporation/selection comments:</li>
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</ul>
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</div>
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<div id="Spore preparation" align="left">
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<h4>Spore preparation</h4>
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Attempt 1:
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<ul>
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<li>Start date/time:</li>
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<li>End date/time:</li>
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<li>Comments:</li>
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</ul>
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</div>
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<div id="Spore transformation" align="left">
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<h4> Spore transformation</h4>
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Attempt 1:
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<ul>
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<li>Start date/time:</li>
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<li>End date/time:</li>
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<li>Comments:</li>
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</ul>
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</div>
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<div id="Evaluation" align="left">
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<h2> Evaluation</h2>
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<ul>
<ul>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/gal4_hap1gr_H2RBmRFP">Full circuit attempt [SSB]</a></li>
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<li>Start date/time:</li>
 +
<li>End date/time:</li>
 +
<li>Comments:</li>
</ul>
</ul>
</div>
</div>
 +
</html>

Revision as of 18:23, 22 July 2014

Gal4 - Hap1GR - H2BmRFP>

Design

Aim of the construct

  • Describe aims here

End goal plasmid

  • insert image here

Experimentation (up to date)

PCR

Attempt 1:

Gibson

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

E-Coli transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Agrobacteria transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Induce comments:
  • Growth comments:
  • Electroporation/selection comments:

Spore preparation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Spore transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Evaluation

  • Start date/time:
  • End date/time:
  • Comments:

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