Team:Wageningen UR

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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<br>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Wageningen_UR&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:Wageningen_UR/Team"style="color:#000000"> Team </a> </td>
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<h1>Save the bananas!</h1>
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<p>iGEM Wageningen UR 2014</p>
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<a href="https://2014.igem.org/Team:Wageningen_UR/Project"style="color:#000000"> Project</a></td>
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<li><a href="background/solution.html">Our Solution</a></li>
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<li><a href="projects.html">Projects</a>
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<li><a href="projects/resistance.html">Resistance</a></li>
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<li><a href="projects/horizontal.html">Horizontal Gene Transfer Prevention</a></li>
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<h1>Overview</h1>
 +
<p>Pathogens belonging to the Fursarium species are known to infect a wide range of crops and cause large losses in agriculture. In this project, we will concentrate on protecting bananas from Fusarium oxysporum f.sp. cubense using an engineered strain of the native soil bacterium Pseudomonas putida. At present commercially grown bananas for export are all the same species, known as Cavendish. Prior to the 1960s, though, the Gros Michel cultivar was the primary banana for export. This species was almost completely wiped out within a decade by the Panama disease that is caused by Fusarium. Recently a new strain of Fusarium has been infecting Cavendish plants and is spreading throughout the world.</p>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<p>The first and perhaps most important part is the production of fungal growth inhibitors. In order to prevent these fungal inhibitors from damaging the soil microbiome, their production will be coupled to the sensing of fusaric acid, a characteristic toxin produced by Fusarium. To help our host compete against the fungus, resistance against fusaric acid will also be incorporated.</p>
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<a href="https://2014.igem.org/Team:Wageningen_UR/Modeling"style="color:#000000"> Modeling</a></td>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<p>Protecting the natural biodiversity is a big part of our project. When the Fusarium threat has been detected and subsequently cleaned up, the bacteria will activate a kill-switch. In addition to this, spread of the genetically modified material will be prevented by a double dependent plasmid system in which loss or gain of one of the two modified plasmids will cause the host to kill itself.</p>
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<a href="https://2014.igem.org/Team:Wageningen_UR/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:Wageningen_UR/Safety"style=" color:#000000"> Safety </a></td>
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<!--<div id="references">
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<h2>References</h2>
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<ol>
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<li>Ploetz, R. C. (1994). "Panama disease: Return of the first banana menace." International Journal of Pest Management 40(4): 326-336.
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<li>Export and production numbers from FAOSTAT 2011 (<a href="www.fao.org">www.fao.org</a>).</li>
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<a href="https://2014.igem.org/Team:Wageningen_UR/Attributions"style="color:#000000"> Attributions </a></td>
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iGEM Wageningen UR 2014. Follow us on <a href="https://twitter.com/igemwageningen">Twitter</a> and <a href="https://www.facebook.com/iGEMWageningen">Facebook</a>!
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For more information about iGEM, check out their <a href="https://igem.org">website</a> and <a href="https://twitter.com/igem">Twitter</a>!
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<ul>
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<li><a href="https://2014.igem.org/Team:Wageningen_UR">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:Wageningen_UR/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Wageningen_UR">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Wageningen_UR/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:Wageningen_UR/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:Wageningen_UR/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:Wageningen_UR/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:Wageningen_UR/Safety">Safety</a> </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Revision as of 12:06, 22 July 2014

Test Page

Overview

Pathogens belonging to the Fursarium species are known to infect a wide range of crops and cause large losses in agriculture. In this project, we will concentrate on protecting bananas from Fusarium oxysporum f.sp. cubense using an engineered strain of the native soil bacterium Pseudomonas putida. At present commercially grown bananas for export are all the same species, known as Cavendish. Prior to the 1960s, though, the Gros Michel cultivar was the primary banana for export. This species was almost completely wiped out within a decade by the Panama disease that is caused by Fusarium. Recently a new strain of Fusarium has been infecting Cavendish plants and is spreading throughout the world.

The first and perhaps most important part is the production of fungal growth inhibitors. In order to prevent these fungal inhibitors from damaging the soil microbiome, their production will be coupled to the sensing of fusaric acid, a characteristic toxin produced by Fusarium. To help our host compete against the fungus, resistance against fusaric acid will also be incorporated.

Protecting the natural biodiversity is a big part of our project. When the Fusarium threat has been detected and subsequently cleaned up, the bacteria will activate a kill-switch. In addition to this, spread of the genetically modified material will be prevented by a double dependent plasmid system in which loss or gain of one of the two modified plasmids will cause the host to kill itself.