Team:Wageningen UR
From 2014.igem.org
Line 1: | Line 1: | ||
{{CSS/Main}} | {{CSS/Main}} | ||
+ | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"> | ||
+ | <head> | ||
+ | <title>Test Page</title> | ||
+ | <meta http-equiv="content-type" content="text/html; charset=utf-8"/> | ||
+ | <meta name="description" content="Test page for wiki"/> | ||
+ | <meta name="author" content="Rik van Rosmalen"/> | ||
+ | |||
+ | <!--<link rel="stylesheet" type="text/css" href="test2.css"/>--> | ||
+ | <style type="text/css"> | ||
+ | /*Hiding default wiki elements - thanks to DTU-Denmark 2011*/ | ||
+ | #contentSub, #search-controls, .firstHeading, #footer-box, #catlinks, #p-logo { | ||
+ | display:none;} | ||
- | + | /* Some changes to the iGEM menu bar - thanks to DTU-Denmark 2011 (https://2011.igem.org/Team:DTU-Denmark/How_to_customize_an_iGEM_wiki) */ | |
+ | #menubar > ul > li:last-child { | ||
+ | display: none; | ||
+ | } | ||
+ | #menubar { | ||
+ | width: auto; | ||
+ | } | ||
+ | body { | ||
+ | margin: 10px 0 0 0; | ||
+ | padding: 0; | ||
+ | } | ||
+ | #top-section{ | ||
+ | background-color: transparent; | ||
+ | } | ||
+ | #menubar a:link, #menubar a:active, #menubar a:visited, #menubar:hover{ | ||
+ | color: transparent; | ||
+ | } | ||
+ | #menubar:hover li a { | ||
+ | color: black; | ||
+ | } | ||
- | + | /*Three Column layout with header and footer */ | |
- | + | * {/* For CSS Reset */ | |
+ | padding: 0; | ||
+ | margin: 0; | ||
+ | } | ||
+ | html { | ||
+ | min-height: 100%; | ||
+ | } | ||
+ | body { | ||
+ | font-family: Verdana, Geneva, sans-serif; | ||
+ | min-width: 1000px; /* 2 x (LC fullwidth + CC padding) + RC fullwidth */ | ||
+ | } | ||
+ | html, body { | ||
+ | height: 100%; | ||
+ | width: 100%; | ||
+ | } | ||
+ | #container{ | ||
+ | display:table; | ||
+ | width: 100%; | ||
+ | height: 100%; | ||
+ | margin: -100px 0 -40px; /*negative header and footer height*/ | ||
+ | padding: 100px 0 40px; /*header and footer height*/ | ||
+ | -moz-box-sizing: border-box; | ||
+ | box-sizing: border-box; | ||
+ | } | ||
+ | #container .column { | ||
+ | display: table-cell; | ||
+ | } | ||
+ | #center { | ||
+ | padding: 10px 20px; /* CC padding */ | ||
+ | box-shadow: 0px 0px 9px rgba(0,0,0,0.15); | ||
+ | } | ||
+ | #left { | ||
+ | width: 200px; /* LC width */ | ||
+ | padding: 0 10px; /* LC padding */ | ||
+ | } | ||
+ | #right { | ||
+ | width: 200px; /* RC width */ | ||
+ | padding: 0 10px; /* RC padding */ | ||
+ | } | ||
+ | #header{ | ||
+ | text-align:center; | ||
+ | font-family: "Courier New", Courier, monospace; | ||
+ | height: 100px; | ||
+ | } | ||
+ | #footer { | ||
+ | font-family:"Courier New", courier, monospace; | ||
+ | font-size:80%; | ||
+ | text-align:center; | ||
+ | height: 40px; | ||
+ | width: 100%; | ||
+ | } | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | + | /*** IE Fix ***/ | |
- | + | * html #left { | |
- | + | left: 150px; /* RC fullwidth */ | |
+ | } | ||
- | + | /* Menu - thanks to tutorial by Chris Spooner (http://line25.com/tutorials/how-to-create-a-pure-css-dropdown-menu)*/ | |
- | + | .menu { | |
+ | margin: 10px auto; | ||
+ | text-align: center; | ||
+ | } | ||
+ | .menu ul ul { | ||
+ | display: none; | ||
+ | } | ||
+ | .menu ul li:hover > ul { | ||
+ | display: block; | ||
+ | } | ||
+ | .menu ul { | ||
+ | background: #efefef; | ||
+ | box-shadow: 0px 0px 9px rgba(0,0,0,0.15); | ||
+ | padding: 0 10px; | ||
+ | margin: 0px; /*Needed to overwrite main igem wiki css*/ | ||
+ | border-radius: 10px; | ||
+ | list-style: none; | ||
+ | position: relative; | ||
+ | display: inline-table; | ||
+ | } | ||
+ | .menu ul:after { | ||
+ | content: ""; | ||
+ | clear: both; | ||
+ | display: block; | ||
+ | } | ||
+ | .menu ul li { | ||
+ | float: left; | ||
+ | margin: 0px; /*Needed to overwrite main igem wiki css*/ | ||
+ | } | ||
+ | .menu ul li:hover { | ||
+ | background: #cfcfcf; | ||
+ | box-shadow: 0px 0px 10px rgba(0,0,0,0.15); | ||
+ | } | ||
+ | .menu ul li:hover a { | ||
+ | color: #fff; | ||
+ | } | ||
+ | .menu ul li a { | ||
+ | display: block; | ||
+ | padding: 30px 15px; /*Menu padding between items*/ | ||
+ | color: #757575; | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | .menu ul ul { | ||
+ | background: #cfcfcf; /*Dropdown background color*/ | ||
+ | box-shadow: 5px 5px 5px rgba(0,0,0,0.15); | ||
+ | border-radius: 0px; | ||
+ | padding: 0; | ||
+ | position: absolute; | ||
+ | top: 100%; | ||
+ | } | ||
+ | .menu ul ul li:first-child { | ||
+ | border-top: 1px solid #6b727c; /*First dropdown option needs a top border also*/ | ||
+ | } | ||
+ | .menu ul ul li { | ||
+ | float: none; | ||
+ | /*border-top: 1px solid #6b727c; - Moved to first child of list so you won't get double lines */ | ||
+ | border-bottom: 1px solid #575f6a; | ||
+ | position: relative; | ||
+ | } | ||
+ | .menu ul ul li a { | ||
+ | padding: 15px 40px; color: #fff; | ||
+ | } | ||
+ | .menu ul ul li a:hover { | ||
+ | background: #4b545f; | ||
+ | } | ||
+ | .menu ul ul ul { | ||
+ | position: absolute; left: 100%; top:0; | ||
+ | } | ||
- | + | #igem_logo{ | |
- | + | height: 100%; | |
- | + | width: 250px; | |
+ | float: right; | ||
+ | } | ||
+ | #center h1,h2{ | ||
+ | margin-bottom:10 px; | ||
+ | padding: 10px; | ||
+ | padding-bottom: 0px; | ||
+ | font-size:150%; | ||
+ | border-bottom: 1px solid black; | ||
+ | } | ||
+ | #center h2{ | ||
+ | font-size:100%; | ||
+ | } | ||
+ | #center p{ | ||
+ | font-size:90%; | ||
+ | } | ||
+ | #center h1+p, h2+p{ | ||
+ | padding-top:10px; | ||
+ | } | ||
- | + | #references{ | |
- | + | font-family: "Courier New",Courier,monospace; | |
+ | } | ||
+ | #references h2{ | ||
+ | font-size:100%; | ||
+ | } | ||
+ | #references ol{ | ||
+ | font-size:75%; | ||
+ | padding-left:30px; | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
- | < | + | <body> |
- | + | ||
- | < | + | <div id="header"> |
- | < | + | <h1>Save the bananas!</h1> |
+ | <p>iGEM Wageningen UR 2014</p> | ||
+ | <div id="igem_logo"> | ||
+ | <a href="https://2014.igem.org"> | ||
+ | <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" alt="" width="50%" /> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
- | < | + | <div id="container"> |
- | + | ||
- | < | + | <div id="left" class="column"> |
- | <a href="https:// | + | <!--<div class="leftmenu"> |
+ | <ul> | ||
+ | <li></li> | ||
+ | </ul> | ||
+ | </div>--> | ||
+ | </div> | ||
+ | |||
+ | <div id="center" class="column"> | ||
+ | <!--<div class="menu"> | ||
+ | <ul> | ||
+ | <li><a href="overview.html">Overview</a> | ||
+ | <ul> | ||
+ | <li><a href="background/background.html">Background</a></li> | ||
+ | <li><a href="background/solution.html">Our Solution</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="projects.html">Projects</a> | ||
+ | <ul> | ||
+ | <li><a href="projects/toxin.html">Fungal Inhibition</a></li> | ||
+ | <li><a href="projects/sensing.html">Sensing</a></li> | ||
+ | <li><a href="projects/resistance.html">Resistance</a></li> | ||
+ | <li><a href="projects/switch.html">Kill Switch</a></li> | ||
+ | <li><a href="projects/horizontal.html">Horizontal Gene Transfer Prevention</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="team.html">Team</a> | ||
+ | <ul> | ||
+ | <li><a href="team/members.html">Members</a></li> | ||
+ | <li><a href="team/acknowledgements.html">Acknowledgements</a></li> | ||
+ | <li><a href="https://igem.org/Team.cgi?id=1493">Team page</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="test.html">Outreach</a> | ||
+ | <ul> | ||
+ | <li><a href="outreach/meeting.html">Dutch iGem Meeting</a></li> | ||
+ | <li><a href="outreach/rivm.html">RIVM</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="safety.html">Safety</a> | ||
+ | <ul> | ||
+ | <li><a href="safety/food.html">Food Safety</a></li> | ||
+ | <li><a href="safety/environment.html">Environment</a></li> | ||
+ | <li><a href="https://igem.org/Team.cgi?id=1493">Safety Form</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="journal.html">Lab Journal</a> | ||
+ | <ul> | ||
+ | <li><a href="journal/journal">Journal</a></li> | ||
+ | <li><a href="journal/protocols">Protocols</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div>--> | ||
+ | <h1>Overview</h1> | ||
+ | <p>Pathogens belonging to the Fursarium species are known to infect a wide range of crops and cause large losses in agriculture. In this project, we will concentrate on protecting bananas from Fusarium oxysporum f.sp. cubense using an engineered strain of the native soil bacterium Pseudomonas putida. At present commercially grown bananas for export are all the same species, known as Cavendish. Prior to the 1960s, though, the Gros Michel cultivar was the primary banana for export. This species was almost completely wiped out within a decade by the Panama disease that is caused by Fusarium. Recently a new strain of Fusarium has been infecting Cavendish plants and is spreading throughout the world.</p> | ||
- | < | + | <p>The first and perhaps most important part is the production of fungal growth inhibitors. In order to prevent these fungal inhibitors from damaging the soil microbiome, their production will be coupled to the sensing of fusaric acid, a characteristic toxin produced by Fusarium. To help our host compete against the fungus, resistance against fusaric acid will also be incorporated.</p> |
- | + | ||
- | < | + | <p>Protecting the natural biodiversity is a big part of our project. When the Fusarium threat has been detected and subsequently cleaned up, the bacteria will activate a kill-switch. In addition to this, spread of the genetically modified material will be prevented by a double dependent plasmid system in which loss or gain of one of the two modified plasmids will cause the host to kill itself.</p> |
- | + | ||
- | < | + | |
- | <a href=" | + | <!--<div id="references"> |
+ | <h2>References</h2> | ||
+ | <ol> | ||
+ | <li>Ploetz, R. C. (1994). "Panama disease: Return of the first banana menace." International Journal of Pest Management 40(4): 326-336. | ||
+ | <li>Export and production numbers from FAOSTAT 2011 (<a href="www.fao.org">www.fao.org</a>).</li> | ||
+ | <ol> | ||
+ | |||
+ | </div>--> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | <div id="right" class="column"> | ||
- | + | </div> | |
- | + | ||
+ | </div> | ||
- | < | + | <div id="footer"> |
- | </ | + | iGEM Wageningen UR 2014. Follow us on <a href="https://twitter.com/igemwageningen">Twitter</a> and <a href="https://www.facebook.com/iGEMWageningen">Facebook</a>! |
- | </ | + | <br /> |
+ | For more information about iGEM, check out their <a href="https://igem.org">website</a> and <a href="https://twitter.com/igem">Twitter</a>! | ||
+ | </div> | ||
- | |||
- | |||
+ | </body> | ||
- | </ | + | </html> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + |
Revision as of 12:06, 22 July 2014
Overview
Pathogens belonging to the Fursarium species are known to infect a wide range of crops and cause large losses in agriculture. In this project, we will concentrate on protecting bananas from Fusarium oxysporum f.sp. cubense using an engineered strain of the native soil bacterium Pseudomonas putida. At present commercially grown bananas for export are all the same species, known as Cavendish. Prior to the 1960s, though, the Gros Michel cultivar was the primary banana for export. This species was almost completely wiped out within a decade by the Panama disease that is caused by Fusarium. Recently a new strain of Fusarium has been infecting Cavendish plants and is spreading throughout the world.
The first and perhaps most important part is the production of fungal growth inhibitors. In order to prevent these fungal inhibitors from damaging the soil microbiome, their production will be coupled to the sensing of fusaric acid, a characteristic toxin produced by Fusarium. To help our host compete against the fungus, resistance against fusaric acid will also be incorporated.
Protecting the natural biodiversity is a big part of our project. When the Fusarium threat has been detected and subsequently cleaned up, the bacteria will activate a kill-switch. In addition to this, spread of the genetically modified material will be prevented by a double dependent plasmid system in which loss or gain of one of the two modified plasmids will cause the host to kill itself.