Team:Oxford/protocols/Streaking Plates

From 2014.igem.org

(Difference between revisions)
EmilyPritchett (Talk | contribs)
(Created page with "__NOTOC__ {{:Team:Oxford/templates/header}} <div class="abcdefg"> <div class="container cf row"> <h1>Plate Streaking</h1> <!--this will number your steps 1,2,3,etc--> <ol...")
Newer edit →

Revision as of 08:49, 22 July 2014



Plate Streaking



    Back to other protocols.

  1. Obtain an LB agar plate.

  2. Label the bottom of the plate.

  3. Carry out the following steps in the lamina flow hood. Using a sterile inoculation loop touch the top of the bacterial stock that is to be plated out.

  4. Gently spread the bacteria over the plate by streaking across one section of the plate. Then drag through the first section to streak over a second section of the plate. Then drag through the second section to streak the third section.

  5. Incubate the plate overnight at the appropriate temperature (30°C for Pseudomonas and 37°C for E. coli).

Retrieved from "http://2014.igem.org/Team:Oxford/protocols/Streaking_Plates"