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Team:BYU Provo/Notebook/CommonProcedures
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Revision as of 00:32, 22 July 2014
BYU 2014 Notebook |
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Q5 PCR Reaction:
10 uL 5x Q5 Reaction Buffer
1 uL dNTPs
1 uL Forward Primer
1 uL Reverse Primer
10 uL Q5 Enhancer
23.5 uL ddH2O
1-2 uL Template
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50 uL Reaction
uL Q5 Polymerase (add right before you start the PCR reaction. Keep on ice until you are ready to do so.)
