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		+	<h><blockquote><b>Q5 PCR Reaction:</b></blockquote></h>
		+	<p><blockquote>10 uL 5x Q5 Reaction Buffer</p></blockquote>
		+	<p><blockquote>1 uL dNTPs</p></blockquote>
		+	<p><blockquote>1 uL Forward Primer</p></blockquote>
		+	<p><blockquote>1 uL Reverse Primer</p></blockquote>
		+	<p><blockquote>10 uL Q5 Enhancer</p></blockquote>
		+	<p><blockquote>23.5 uL ddH2O</p></blockquote>
		+	<p><blockquote>1-2 uL Template</p></blockquote>
		+	<p><blockquote>---------------</p></blockquote>
		+	<p><blockquote>50 uL Reaction</blockquote></p>
		+	<br></br>
		+	<p><blockquote> uL Q5 Polymerase (add right before you start the PCR reaction. Keep on ice until you are ready to do so.)</blockquote></p>
		+
		+
		+
		+	<br></br>
	</html>		</html>

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Revision as of 22:38, 21 July 2014

BYU 2014 Notebook EDIT Procedures

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Q5 PCR Reaction:

10 uL 5x Q5 Reaction Buffer

1 uL dNTPs

1 uL Forward Primer

1 uL Reverse Primer

10 uL Q5 Enhancer

23.5 uL ddH2O

1-2 uL Template

---------------

50 uL Reaction

uL Q5 Polymerase (add right before you start the PCR reaction. Keep on ice until you are ready to do so.)