Team:Paris Saclay/Notebook/July/9
From 2014.igem.org
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* Instructors and advisors: Alice, Olivier, Solenne and Sylvie. | * Instructors and advisors: Alice, Olivier, Solenne and Sylvie. | ||
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] |
Revision as of 21:12, 21 July 2014
Contents |
Wednesday 9th July
Lab work
1 - Plasmid DNA Extraction:
Romain & Sean
Plasmids used: DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A and B.
2 - Electrophoresis:
Arnaud & Romain & Sean
Plasmids used:
- DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A.
- DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, B.
- XL1-Blue pCola-ges Kan
- pJBEI-6409 Cm
3 - New culture:
Romain
New liquid cultures of:
- FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
- BBa B0015 dble ter P5B1C3 Cm
4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.
Reunion
Conference with the team to discuss about the project. The handled topics were:
- Definition of Biobricks to make
- Planning of internships
- Definition of Biobricks to render
- Understanding the construction of consecutive genes
- Organisation and planning of experiences to do
- Sponsoring
- Wiki's progress
- Modelling's progress
Members present:
- Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.