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Team:Paris Saclay/Notebook/July/21
From 2014.igem.org
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==Lab Work== | ==Lab Work== | ||
| - | ===1 - Results: Transformation of supercompetent cells with | + | ===1 - Results: Transformation of supercompetent cells with CaCl<sub>2</sub>=== |
''by Romain'' | ''by Romain'' | ||
Revision as of 20:19, 21 July 2014
Contents |
Monday 21st July
Lab Work
1 - Results: Transformation of supercompetent cells with CaCl2
by Romain
Strains transformed: MG1655, MG1655Z1. from Bacterial Cultures transformed the 18th July
Results: Nothing has grown.
2 - Oligo's design
by Romain
3 - Liquid Bacterial Culture
by Marie, Romain & Sean
- DY330 pJBEI6409 with 10µl Cm in 10ml LB (x2)
- BT340 Cm and Amp
- The new strains received
4 - Electrophoresis
by Fabio (process A) and Mathieu (process B and C)
We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel. The PCRs came from Mathias' manipulation made the 18th July.
Process A
- J23119 Cl1
- J23119 Cl2
- J23106 Cl1
- J23100 Cl2
- PCR 1
- PCR 2
- PCR 3
- PCR 4
- PCR 5
- PCR 6
- PCR 7
- PCR 8
- PCR 9
- PCR 10
Process B
Process C
Pooling and purifying PCR 9 and 10 from process A.
- PCR purified products. IPS70 / IPS71 of pOsV230 (Apramycin)
- BT 340 (plasmid's flipase)
Results:
- A: From 1 to 4: Success, DNAs have the expected size.
- A: From 5 to 12: Failure, No PCR products.
- A: Numbers 13 and 14: Success, PCR products have the expected size.
- B: All 6 extractions were successful.
- C: Number 1: successful concentration of pOsV230's PCR product.
- C: Number 2 had no migration.
People there:
- Instructors and advisors: Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
