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Team:Gifu/Protocol
From 2014.igem.org
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$("tr:even").addClass("odd"); | $("tr:even").addClass("odd"); | ||
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</head> | </head> | ||
<body> | <body> | ||
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<h1 id="theme"><a name="top"></a>Protocols</h1> | <h1 id="theme"><a name="top"></a>Protocols</h1> | ||
<ul class="list"> | <ul class="list"> | ||
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| + | <li><a href="#RD">Media</a></li> | ||
<li><a href="#RD">Restriction Digests</a></li> | <li><a href="#RD">Restriction Digests</a></li> | ||
<li><a href="#LI">Ligation</a></li> | <li><a href="#LI">Ligation</a></li> | ||
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<li><a href="#SDS">SDS-PAGE</a></li> | <li><a href="#SDS">SDS-PAGE</a></li> | ||
</ul> | </ul> | ||
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<div id="contents"> | <div id="contents"> | ||
<h2><a name="RD"></a>Restriction Digests</h2> | <h2><a name="RD"></a>Restriction Digests</h2> | ||
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<h2><a name="CP"></a>Colony PCR</h2> | <h2><a name="CP"></a>Colony PCR</h2> | ||
<ol> | <ol> | ||
| - | <li>Collect a colony from a plate and then suspend it in 100 µL of LB | + | <li>Collect a colony from a plate and then suspend it in 100 µL of LB media (liquid). </li> |
<li>Mix the following reagents.</br> | <li>Mix the following reagents.</br> | ||
<table class="table" border="2"> | <table class="table" border="2"> | ||
Latest revision as of 02:10, 28 August 2015
