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Team:Sumbawagen/Notebook/protocol5
From 2014.igem.org
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<a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="https://igem.org"><img width="70px" height="50px" src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png"> </a> | <a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="https://igem.org"><img width="70px" height="50px" src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png"> </a> | ||
| - | <a class="brand" href="https://2014.igem.org/Team:Sumbawagen | + | <a class="brand" href="https://2014.igem.org/Team:Sumbawagen">Sumbawagen</a> |
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<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/ | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li> |
</ul> | </ul> | ||
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<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li> | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li> |
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li> | ||
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<a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook"> | + | |
| + | <li class="nav-header">Daily Notes</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook3">Mobile Programming</a></li> | ||
| + | <li class="divider"></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li> | ||
<!--<li><a href="#">Improvements</a></li>--> | <!--<li><a href="#">Improvements</a></li>--> | ||
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<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li> | ||
| - | |||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li> | ||
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support"> | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li> |
Revision as of 03:09, 19 February 2015
Team:Sumbawagen/Team
From 2014.igem.org
Notebook – Protocol – 5. Restriction enzymes digestion
1. Restriction enzymes digestion was done using Fast Digest product (Thermoscientific) using the following composition:
- DNA sample (1-3 ug), 15 ul
- Fast Digest restriction enzyme #1, 2 ul
- Fast Digest restriction enzyme #2, 2 ul
- 10x buffer, 3 ul
- Sterilized distilled water, 6 ul Total volume, 30 ul
2. Samples were incubated at 37 °C for more than 1 hour (typically 3 hours).
3. When necessary, samples were electrophoresed using protocol “3. Electrophoresis”(typically 3 hours).
4. Samples were purified using protocol “4. DNA purification”.
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