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Team:Sumbawagen/Notebook/protocol11
From 2014.igem.org
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<p>2. The pre-culture was shaken over-night at room temperature.</p> | <p>2. The pre-culture was shaken over-night at room temperature.</p> | ||
<p>3. Main culture was prepared for the next day, which contains pre-culture, LB medium, Cam, expression reagent for the expression of reporter gene mRFP (autoinduction medium which includes 20x NPS, 50x 5052 and 1,000x Mg), sugar for catabolite repression induction (either glucose, fructose, glucose+fructose, or honey). Detail receipt with various concentration of sugars was given in Tables below.</p> | <p>3. Main culture was prepared for the next day, which contains pre-culture, LB medium, Cam, expression reagent for the expression of reporter gene mRFP (autoinduction medium which includes 20x NPS, 50x 5052 and 1,000x Mg), sugar for catabolite repression induction (either glucose, fructose, glucose+fructose, or honey). Detail receipt with various concentration of sugars was given in Tables below.</p> | ||
| - | <p> | + | <p>4. Main culture was shaken over-night at room temperature. </p> |
<center><img src="https://static.igem.org/mediawiki/2014/d/d2/11_1.png"><br></center><br> | <center><img src="https://static.igem.org/mediawiki/2014/d/d2/11_1.png"><br></center><br> | ||
<center><img src="https://static.igem.org/mediawiki/2014/a/ae/11_2.png"><br></center><br> | <center><img src="https://static.igem.org/mediawiki/2014/a/ae/11_2.png"><br></center><br> | ||
Revision as of 03:03, 19 February 2015
Team:Sumbawagen/Team
From 2014.igem.org
Notebook – Protocol – 11. Cultivation
1. From plate of E. coli BL21/DE3 harboring appropriate plasmid, colony was picked and cultured in 2 ml LB medium containing antibiotic chloramphenicol (Cam) with final concentration of 34 ug/ml. 10 ml size glass tube was used. This was pre-culture.
2. The pre-culture was shaken over-night at room temperature.
3. Main culture was prepared for the next day, which contains pre-culture, LB medium, Cam, expression reagent for the expression of reporter gene mRFP (autoinduction medium which includes 20x NPS, 50x 5052 and 1,000x Mg), sugar for catabolite repression induction (either glucose, fructose, glucose+fructose, or honey). Detail receipt with various concentration of sugars was given in Tables below.
4. Main culture was shaken over-night at room temperature.
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