Team:UT-Dallas/temp

From 2014.igem.org

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<section id="titlechart"></html>{{Header_menu}}<html><div class="page_content"><br><h2>Safety</H2><p style="display:block">
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<section id="titlechart"></html>{{Header_menu}}<html><div class="page_content"><br><h2>Parts</H2><p style="display:block">
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<br> Before beginning work on the project, every lab member underwent training with the Advisor on various safety protocols and proper techniques to be followed in lab, including how to properly handle biological organisms and parts, how to use equipment safely, measures to keep the lab area clean and precautions when using carcinogenic materials such as ethidium bromide. Students were advised to wear long pants, close-toes shoes, and appropriate personal protective equipment. This training followed the requirements outlined in the laboratory safety training requirement of UT Dallas, which can be found at http://www.utdallas.edu/research/orc/lab_safety_training.
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<br><img src="https://static.igem.org/mediawiki/2014/4/48/GRNA_Library.png">
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E. coli strain DH5a was acquired from NEB and used as a chassis throughout the project. The species falls under Risk Group 1 as classified by the American Biological Safety Association (ABSA) and can potentially cause skin and eye irritation in humans. Had our project progressed to further stages, the use of Vibrio cholerae would have been required, which is in Risk Group 2 and poses greater risk to humans, requiring more precaution in handling it.
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<br><h2>Local Rules and Regulations </H2><br><p style="display:block">
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<br><h2>Parts List</H2><br><p style="display:block">
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<font color="black" style="font-weight:bold;font-size:20px">Who is responsible for biological safety at your institution?</font>
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<table style="border:1px solid black;">
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<br> Sanaz Okhovat and Shane Solis at the Office of Research Compliance are in charge of biology safety at UTD. <br><br>
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<tbody><tr style="border:1px solid black;">
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<font color="black" style="font-weight:bold;font-size:20px"> What are the biosafety guidelines of your institution? Please give a link to these guidelines. </font>
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<td style="border:1px solid black;">Name</td>
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<br> <a href="http://www.utdallas.edu/ehs/manuals/docs/BioSafety%20Manual.pdf" style="font-size:20px">http://www.utdallas.edu/ehs/manuals/docs/BioSafety%20Manual.pdf</a><br><br>
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<td style="border:1px solid black;">Type</td>
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<font color="black" style="font-weight:bold;font-size:20px"> In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations.</font>
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<td style="border:1px solid black;">Description</td>
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<br><a href="http://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf " style="font-size:20px">http://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf </a>
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<td style="border:1px solid black;">Length</td>
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<br><br>
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</tr>
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<tr style="border:1px solid black;">
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487021"> BBa_K1487021</a> </td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfA gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487022"> BBa_K1487022</a> </td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfB gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487023"> BBa_K1487023</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfC gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487024"> BBa_K1487024</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfD gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487025"> BBa_K1487025</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-ctxA gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487026"> BBa_K1487026</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-ctxB gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487027"> BBa_K1487027</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpA gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487028"> BBa_K1487028</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpB gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487029"> BBa_K1487029</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpC gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487030"> BBa_K1487030</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpD gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487031"> BBa_K1487031</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpE gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487032"> BBa_K1487032</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpF gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487033"> BBa_K1487033</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpH gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487034"> BBa_K1487034</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpI gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487035"> BBa_K1487035</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpJ gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487036"> BBa_K1487036</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpP gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487037"> BBa_K1487037</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpQ gRNA</td><td style="border:1px solid black;">143</td>
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</tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487038"> BBa_K1487038</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpR gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487039"> BBa_K1487039</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpS gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487040"> BBa_K1487040</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpT gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487041"> BBa_K1487041</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-toxT gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487063"> BBa_K1487063</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfA) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487064"> BBa_K1487064</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfB) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487065"> BBa_K1487065</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfC) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487066"> BBa_K1487066</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfD) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487067"> BBa_K1487067</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(ctxA) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487068"> BBa_K1487068</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(ctxB) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487069"> BBa_K1487069</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpA) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487070"> BBa_K1487070</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpB) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487071"> BBa_K1487071</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpC) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487072"> BBa_K1487072</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpD) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487073"> BBa_K1487073</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpE) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487074"> BBa_K1487074</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpF) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487075"> BBa_K1487075</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpH) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487076"> BBa_K1487076</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpI) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487077"> BBa_K1487077</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpJ) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487078"> BBa_K1487078</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpP) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487079"> BBa_K1487079</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpQ) - YFP</td><td style="border:1px solid black;">805</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487080"> BBa_K1487080</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpR) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487081"> BBa_K1487081</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpS) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487082"> BBa_K1487082</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpT) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487083"> BBa_K1487083</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(toxT) - YFP</td><td style="border:1px solid black;">807</td>
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</tr></tbody></table>
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<br><h2>Risks of the Project </H2><br><p style="display:block">
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<font color="black" style="font-weight:bold;font-size:20px">Risks to the safety and health of team members, or other people working in the lab:</font>
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E. coli is a frequently used organism on lab, which carries risk of lab-acquired infection since there are inherent risks of the organism spreading in the lab if it is not properly handled and contamination occurs.
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<br><br><font color="black" style="font-weight:bold;font-size:20px">Risks to the safety and health of the general public (if any biological materials escaped from your lab):</font><br>
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In the worst-case scenario, an antibiotic-resistant form of E. coli escapes lab and ends up the water source, where the likelihood of infection increases significantly and the bacteria is not responsive to the antibiotics administered for treatment.
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<br><br><font color="black" style="font-weight:bold;font-size:20px">Risks to the environment (from waste disposal, or from materials escaping from your lab):</font><br>
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If the organisms are not disposed of properly and end up outside of the work environment, the E. coli may end up in the water sources and affect the local ecosystems. In such a case, the organisms may interact with the bacteria present in the sewer systems and affect the native bacterial population.
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<br><br><font color="black" style="font-weight:bold;font-size:20px">Risks to security through malicious mis-use by individuals, groups, or countries:</font><br>
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In the hands of someone with malicious intent, the bacteria may be purposefully released into the environment. To minimize this risk, all experiments will be carried out in an area that is secured against unauthorized entry. The building in which our lab is located is also under constant surveillance.
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<br><br><font color="black" style="font-weight:bold;font-size:20px">What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)</font><br>
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The project, which targets various cholera genes, will be carried out in E. coli and the use of V. cholerae will be extremely limited, if at all ordered. We will take caution in handling organisms by limiting movement to the area and use personal protection equipment such as gloves and lab coats. Actions such as frequently changing gloves and cleaning the workbench with alcohol will be frequently implemented. By using efficient lab techniques and safe practices, we will keep the lab and the students working in it as safe as possible.
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<br><br><font color="black" style="font-weight:bold;font-size:20px">What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</font><br>
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We will demonstrate our model in E. coli, but our ideal final product would be a common probiotic bacterium, which poses very little risk to the lab workers, the public, or the environment. Ideally, our end product would contain a lytic phage specific for Vibrio, creating very little risk to the public and natural gut flora. It is possible for someone with ill intentions to alter our system and cause harm.
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<br><br><font color="black" style="font-weight:bold;font-size:20px">Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</font>
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Yes, one aspect of the project will involve using a lytic phage under an inducible promoter. This would kill our engineered E. coli.
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Revision as of 03:46, 18 October 2014


Parts





Parts List


Name Type Description Length
BBa_K1487021 CompositeP-acfA gRNA145
BBa_K1487022 CompositeP-acfB gRNA145
BBa_K1487023 CompositeP-acfC gRNA145
BBa_K1487024 CompositeP-acfD gRNA145
BBa_K1487025 CompositeP-ctxA gRNA145
BBa_K1487026 CompositeP-ctxB gRNA145
BBa_K1487027 CompositeP-tcpA gRNA145
BBa_K1487028 CompositeP-tcpB gRNA145
BBa_K1487029 CompositeP-tcpC gRNA145
BBa_K1487030 CompositeP-tcpD gRNA145
BBa_K1487031 CompositeP-tcpE gRNA145
BBa_K1487032 CompositeP-tcpF gRNA145
BBa_K1487033 CompositeP-tcpH gRNA145
BBa_K1487034 CompositeP-tcpI gRNA145
BBa_K1487035 CompositeP-tcpJ gRNA145
BBa_K1487036 CompositeP-tcpP gRNA145
BBa_K1487037 CompositeP-tcpQ gRNA143
BBa_K1487038 CompositeP-tcpR gRNA145
BBa_K1487039 CompositeP-tcpS gRNA145
BBa_K1487040 CompositeP-tcpT gRNA145
BBa_K1487041 CompositeP-toxT gRNA145
BBa_K1487063 CompositeP + t.seq(acfA) - YFP807
BBa_K1487064 CompositeP + t.seq(acfB) - YFP807
BBa_K1487065 CompositeP + t.seq(acfC) - YFP807
BBa_K1487066 CompositeP + t.seq(acfD) - YFP807
BBa_K1487067 CompositeP + t.seq(ctxA) - YFP807
BBa_K1487068 CompositeP + t.seq(ctxB) - YFP807
BBa_K1487069 CompositeP + t.seq(tcpA) - YFP807
BBa_K1487070 CompositeP + t.seq(tcpB) - YFP807
BBa_K1487071 CompositeP + t.seq(tcpC) - YFP807
BBa_K1487072 CompositeP + t.seq(tcpD) - YFP807
BBa_K1487073 CompositeP + t.seq(tcpE) - YFP807
BBa_K1487074 CompositeP + t.seq(tcpF) - YFP807
BBa_K1487075 CompositeP + t.seq(tcpH) - YFP807
BBa_K1487076 CompositeP + t.seq(tcpI) - YFP807
BBa_K1487077 CompositeP + t.seq(tcpJ) - YFP807
BBa_K1487078 CompositeP + t.seq(tcpP) - YFP807
BBa_K1487079 CompositeP + t.seq(tcpQ) - YFP805
BBa_K1487080 CompositeP + t.seq(tcpR) - YFP807
BBa_K1487081 CompositeP + t.seq(tcpS) - YFP807
BBa_K1487082 CompositeP + t.seq(tcpT) - YFP807
BBa_K1487083 CompositeP + t.seq(toxT) - YFP807



Retrieved from "http://2014.igem.org/Team:UT-Dallas/temp"