Team:Evry/Notebook/Sensing/Phenol/09-30-2014
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<u> Mutation of the Pst1 site </u> <br> | <u> Mutation of the Pst1 site </u> <br> | ||
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- | Digestion of plasmid containing DmpR + GFP on three strains of <i>E.coli</i> ( | + | Digestion of plasmid containing DmpR + GFP on three strains of <i>E.coli</i> (DH5a, Top10 and Bl21) by EcoR1 (wells 1), Pst1 (wells 2) and EcoR1+Pst1 (wells 3). <br> |
<img src="https://static.igem.org/mediawiki/2014/2/26/30.09annot%C3%A9.jpg"/> <br> | <img src="https://static.igem.org/mediawiki/2014/2/26/30.09annot%C3%A9.jpg"/> <br> | ||
+ | e have the good construction in DH5a AND tOP 10 STRAINS. <BR> | ||
<br> | <br> |
Revision as of 03:27, 18 October 2014
Mutation of the Pst1 site
Digestion of plasmid containing DmpR + GFP on three strains of E.coli (DH5a, Top10 and Bl21) by EcoR1 (wells 1), Pst1 (wells 2) and EcoR1+Pst1 (wells 3).
e have the good construction in DH5a AND tOP 10 STRAINS.