Team:ZJU-China/Safety
From 2014.igem.org
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<td>DH5-alpha</td> | <td>DH5-alpha</td> | ||
<td>1</td> | <td>1</td> | ||
- | <td>Yes.May cause irritation to skin, eyes, and respiratory tract | + | <td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td> |
</td> | </td> | ||
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<td>BL21(DE3)</td> | <td>BL21(DE3)</td> | ||
<td>1</td> | <td>1</td> | ||
- | <td>Yes.May cause irritation to skin, eyes, and respiratory tract | + | <td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td> |
</tr> | </tr> | ||
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<td>DH10-beta</td> | <td>DH10-beta</td> | ||
<td>1</td> | <td>1</td> | ||
- | <td>Yes.May cause irritation to skin, eyes, and respiratory tract | + | <td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td> |
</tr> | </tr> | ||
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<td>BW25113</td> | <td>BW25113</td> | ||
<td>1</td> | <td>1</td> | ||
- | <td>Yes.May cause irritation to skin, eyes, and respiratory tract | + | <td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<ol> | <ol> | ||
<li><b>Lab Safety</b> | <li><b>Lab Safety</b> | ||
- | <p> | + | <p>Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required to wear protective equipments including lab-gown, long pants, gloves etc, while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.</p> |
</li> | </li> | ||
<li><b>Research Safety</b> | <li><b>Research Safety</b> | ||
- | <p>All students involved | + | <p>All students involved have been trained to operate equipments, reagents and strains in a safe way. We are not able to do ecperiments until we pass the bio-safety examination.</p> |
</li> | </li> | ||
<li><b>Environment Safety</b> | <li><b>Environment Safety</b> | ||
- | <p> All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used | + | <p> All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don't contain F plasmid, which guarantees that our genes would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.</p> |
</li> | </li> | ||
<li><b>Reference</b> | <li><b>Reference</b> |
Revision as of 03:16, 18 October 2014
Species | Strain | NIH Risk Group | Disease risk to human? |
---|---|---|---|
E.Coli(K12) | DH5-alpha | 1 | Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys. |
E.Coli(K12) | BL21(DE3) | 1 | Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys. |
E.Coli(K12) | DH10-beta | 1 | Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys. |
E.Coli(K12) | BW25113 | 1 | Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys. |
Our Safety Protocol
- Lab Safety
Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required to wear protective equipments including lab-gown, long pants, gloves etc, while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.
- Research Safety
All students involved have been trained to operate equipments, reagents and strains in a safe way. We are not able to do ecperiments until we pass the bio-safety examination.
- Environment Safety
All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don't contain F plasmid, which guarantees that our genes would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.
- Reference
http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia