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Team:Imperial/Results
From 2014.igem.org
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<li><a data-scroll href="#overview">Overview</a> | <li><a data-scroll href="#overview">Overview</a> | ||
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| - | <li><a data-scroll href="#result1"> | + | <li><a data-scroll href="#result1">G. xylinus</a> |
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<li><a data-scroll href="#result2">RESULT 2</a> | <li><a data-scroll href="#result2">RESULT 2</a> | ||
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<section id="result1"> | <section id="result1"> | ||
| - | <h2> | + | <h2>G.xylinus</h2> |
| - | <p> | + | <h3>Overview</h3> |
| + | <p>Bacterial cellulose has great potential in many areas, including water purification, tissue scaffolds, wound dressings, etc., however, until now, all bacterial cellulose-based materials have been created using chemical or physical post-production processing, not genetic engineering. This is due to the lack of well-developed tools and methods for Gluconacetobacter genetic engineering, as well as the lack of genome sequence of the highest cellulose-producing strain ATCC 53582. We have overcome the numerous difficulties associated with G.xylinus genetic engineering, and turned G.xylinus KI and ATCC 53852 strains into new platforms for the production of cellulose-based biomaterials by sequencing the genomes of ATCC 53582 and KI, creating a genetic toolbox of consisting of five new plasmid backbones and around 40 widely used genes, and developing a set of new and improved protocols for G.xylinus genetic engineering.</p> | ||
| + | <h3>Key Achievements</h3> | ||
| + | <ul> | ||
| + | <li>Isolated a new strain of <em>Gluconacetobacter</em> (named <em>G. xylinus</em> igem) from Kombucha tea and characterized its properties fully.</li> | ||
| + | <li>Sequenced the previously unknown genomes of <em>G. xylinus</em> ATCC 53582 and <em>G. xylinus</em> igem strains - the first genomes sequenced in the history of iGEM </li> | ||
| + | <li>Discovered four new plasmids capable of replication in <em>Gluconacetobacter</em> species - pSEVA321, pSEVA331, pSEVA351 and pBAV1K, which replicate both in <em>G. xylinus</em> ATCC 53582 and igem strains as well as in <em>E. coli</em></li> | ||
| + | <li>Were the first in science to create transgenic cells of <em>G.xylinus</em> igem strain </li> | ||
| + | <li>Using our discovered plasmids, created a genetic toolbox consisting of 40 genes for <em>G. xylinus</em> engineering and expressed them in the ATCC 53582 and igem</li> | ||
| + | |||
| + | <li>Developed a set of new and improved protocols for efficient genetic engineering of <em>G. xylinus</em></li> | ||
| + | <li>In summary, turned <em>G. xylinus</em> ATCC 53582 and igem strains into new model organisms and developed the necessary tools to create a powerful platform for the synthesis of new cellulose-based biomaterials and water filters</li> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </ul> | ||
</section> | </section> | ||
Revision as of 03:13, 18 October 2014
Results
Overview
Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.Much Text. Such prose. Wow.
G.xylinus
Overview
Bacterial cellulose has great potential in many areas, including water purification, tissue scaffolds, wound dressings, etc., however, until now, all bacterial cellulose-based materials have been created using chemical or physical post-production processing, not genetic engineering. This is due to the lack of well-developed tools and methods for Gluconacetobacter genetic engineering, as well as the lack of genome sequence of the highest cellulose-producing strain ATCC 53582. We have overcome the numerous difficulties associated with G.xylinus genetic engineering, and turned G.xylinus KI and ATCC 53852 strains into new platforms for the production of cellulose-based biomaterials by sequencing the genomes of ATCC 53582 and KI, creating a genetic toolbox of consisting of five new plasmid backbones and around 40 widely used genes, and developing a set of new and improved protocols for G.xylinus genetic engineering.
Key Achievements
- Isolated a new strain of Gluconacetobacter (named G. xylinus igem) from Kombucha tea and characterized its properties fully.
- Sequenced the previously unknown genomes of G. xylinus ATCC 53582 and G. xylinus igem strains - the first genomes sequenced in the history of iGEM
- Discovered four new plasmids capable of replication in Gluconacetobacter species - pSEVA321, pSEVA331, pSEVA351 and pBAV1K, which replicate both in G. xylinus ATCC 53582 and igem strains as well as in E. coli
- Were the first in science to create transgenic cells of G.xylinus igem strain
- Using our discovered plasmids, created a genetic toolbox consisting of 40 genes for G. xylinus engineering and expressed them in the ATCC 53582 and igem
- Developed a set of new and improved protocols for efficient genetic engineering of G. xylinus
- In summary, turned G. xylinus ATCC 53582 and igem strains into new model organisms and developed the necessary tools to create a powerful platform for the synthesis of new cellulose-based biomaterials and water filters
HEADLINE RESULT OVERVIEW 2
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HEADLINE RESULT OVERVIEW 3
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HEADLINE RESULT OVERVIEW 4
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HEADLINE RESULT OVERVIEW 5
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