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Team:Bordeaux/NotebookSeptember25
From 2014.igem.org
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- Sodium sulfate (Na2SO4) to 320g / L fiber => a fluffy appearance that does not take. <br> | - Sodium sulfate (Na2SO4) to 320g / L fiber => a fluffy appearance that does not take. <br> | ||
| - | - Diethyl ether (C2H5) 2O | + | - Diethyl ether (C2H5) 2O => no reaction because the drop is trapped we are finding that the solvent is immiscible. <br> |
| - | - Acetone (CH3COCH3) | + | - Acetone (CH3COCH3) =>we observe the formation of a white ring.<br> |
| - | - Sodium chloride (NaCl 5M) hot | + | - Sodium chloride (NaCl 5M) hot => observation of a white cloud, which is due to a problem of cohesiveness.<br> |
| - | - Calcium chloride (CaCl2 15%) | + | - Calcium chloride (CaCl2 15%) => we do a mixture of 1.25% alginate and ELP 40 (10 mg / ml) => fiber forming a perfect white.<br> </div><br><br> |
NB: test 0.75% of alginate PERFECT! | NB: test 0.75% of alginate PERFECT! | ||
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<li>Mechanical traction device <br><br> | <li>Mechanical traction device <br><br> | ||
Latest revision as of 03:05, 18 October 2014
4th June
Molecular biology
- ELP characterization
- Test of polymere formation
To get the thread of polymers we have tested in different polymers we observed the following results :
- Sodium sulfate (Na2SO4) to 320g / L fiber => a fluffy appearance that does not take.
- Diethyl ether (C2H5) 2O => no reaction because the drop is trapped we are finding that the solvent is immiscible.
- Acetone (CH3COCH3) =>we observe the formation of a white ring.
- Sodium chloride (NaCl 5M) hot => observation of a white cloud, which is due to a problem of cohesiveness.
- Calcium chloride (CaCl2 15%) => we do a mixture of 1.25% alginate and ELP 40 (10 mg / ml) => fiber forming a perfect white.
NB: test 0.75% of alginate PERFECT! -
Test of transition
After obtaining the fiber ELP 40 it is necessary to wash with hot water and extract; fiber may dissolve cold.
- The control is transparent
- The fiber changes color on contact at room Temperature, passing from white to transparent
With the mixture Alginate ELP 40 + (10mg / L) we can observe the formation of the polymer and we can infer that :
- In the presence of salt = the product disintegrates
- In contact with water this contracts = warm
- Wetspenning
- Mechanical test :
The traction machine can measure the degree of resistance to the breakage of any material.
This experiment involves placing a small bar of the material to be studied between the jaws of a tensile testing machine that draws on the bar until it breaks. Elongation was recorded and the force applied, which is then converted into stress and strain. - Mechanical traction device
The mechanical tests allow us to say :- - Alginate is reproducible but ends up breaking after a time t
- Alginate breaks faster due to the fact that it was realized hot
- We see a similar pattern, just an anomaly on the curve of a slip of paper without doubts. - - Elasticity similar to that of Carbon nano which is due to the presence of polymers, so we have a very obvious ELP particular feature.
- Fragile fibers that break easily
Sample 10mg
Breaks quickly because there MAYBE too much material, so the sample 2-3, 10-1 are not taken into account for the results!
- - Alginate is reproducible but ends up breaking after a time t
- 2nd mechanical tests
Different samples of ELP
Sample 1.2 = with more alginate we don’t notice anything important, it breaks fast
1.1 => resisted longer before breaking but there is an anomaly that is the glue that gives this end there
1.1 B => Strange results due to different conditions of drying?
- Test of polymere formation
