Team:BNU-China/home.html

From 2014.igem.org

(Difference between revisions)

Revision as of 03:02, 18 October 2014

1 2

We are iGEMers from BNU-China, we are going to tell you a story about E.coli Prometheus ...

Mr E.coli Prometheus is a philanthropic man. One day, he saw plenty poor peanuts living in a condition that was rough for them to grow up nor to flower. He made up his mind that he must to do something to help!

Then he found us for help, he asked for the power to collect molybdate and send them to peanut. We told him: " Since the power is not belong to you in nature, for the sake of the other life, you will die after this." But he didn't change his mind for one second.

E.coli Prometheus collected many molybdate with the power ...

He sent the precious micronutrient to those peanut everyday. The peanuts became more and more healthier and was about to flower ...

Sadly, E.coli Prometheus flied to heaven before he saw the flowers of peanut. He died as we informed him before. His body changed into nutrition for peanut after his death.
He is honorable.

THE STORY OF E.COLI PROMETHEUS

Our work

The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots. We transferred our designed plasmids into E.coli "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA,absorbs molybdate. With the help of organic acid chemotaxis system, our "Prometheus" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. E.coli Prometheus will deliver Mo directly to the root. This opens a new chapter of biological fertilizer.

Wiki Design

Art design, pictures, figures and the latest experiment results were put on the wiki in time. Wiki is an important platform to show our works.

ModA & INPN

ModA we used in our project is a kind of protein that can bind the molybdate and INPN can display ModA on E.coli's surface.

Chemotaxis

We Used McfR plasmid to give the E.coli the capacity of Chemotaxis, so that those E.coli with molybdate can swim to the root of peanut.

Modeling

We have made the model of peanut root and E.coli in environment to show how our thought be realized out of lab.

Latest Works

Prev Next

The Story of E.coli Prometheus

BNU-China

@@ Line 61: / Line 61: @@
      <h2>Our work</h2>
      <div class="two-third">
-       <p>Nitrogen fixing ability of rhizobium is significant for the growth of legumes. The stronger of its ability, the less chemical fertilizer will be used. Molybdenum is a key integral part of nitrogen fixing enzyme in rhizobium and Mo is also important for the growth of plants themselves. However, Mo is heavy mental. The abuse of it will cause environment pollution. Hence, spot application of Mo is of great importance. A plasmid was transfer into <i>E.coli</i> Prometheus to make it express fusion protein, one side of which anchors on the out membrane and the other side of which,ModA,catches molybdate. With the help of organic acid tendency system, our Prometheus will head for the roots. A suicide system depending on time is also design for the reason of bio-safety. <i>E.coli Prometheus</i> will deliver Mo directly to the root of plants. This will also open a new chapter of biological fertilizer.
+       <p>The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots.
+We transferred our designed plasmids into E.coli "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA,absorbs molybdate. With the help of organic acid chemotaxis system, our "Prometheus" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns.   E.coli Prometheus will deliver Mo directly to the root. This opens a new chapter of biological fertilizer.
      </div>    <!-- End Top Columns -->