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Team:Paris Saclay/Notebook/July/18
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from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July] | from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July] | ||
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| + | ===2 - pJBEI 6409 plasmid construction with apramycin=== | ||
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| + | ''by Sean'' | ||
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| + | Optical density of .134 measured at 10:40am, .619 at 12:30pm. | ||
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| + | '''''Electroporation''''' | ||
| + | Three samples with varying amounts of plasmids: control, 2μL, 4μL | ||
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| + | Protocol | ||
| + | |||
| + | (will check this on Monday... don't edit this part! - SC) | ||
Revision as of 19:55, 20 July 2014
Contents |
Friday 18th July
Lab work
1 - Transformation of supercompetent cells with CaCl2
by Romain & Arnaud
Strains transformed: MG1655, MG1655Z1. from Bacterial Cultures made the 17th July
2 - Plasmid DNA Purification
by Fabio
- J23119 (x2)
from Bacterial Culture made the 17th July
2 - pJBEI 6409 plasmid construction with apramycin
by Sean
Optical density of .134 measured at 10:40am, .619 at 12:30pm.
Electroporation Three samples with varying amounts of plasmids: control, 2μL, 4μL
Protocol
(will check this on Monday... don't edit this part! - SC)
People there:
- Instructors and advisors: Solenne.
- Students: Arnaud, Fabio, Mathias, Romain and Sean.
