Team:British Columbia/ProjectBiomining

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'''Our solution: Mineral Separation''':
'''Our solution: Mineral Separation''':
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As a proof of concept, we have chosen to express these peptides to selectively bind to chalcopyrite, which will then fall out of solution by aggregation using two interacting coiled-coil peptides expressed on the S-layer of ''Caulobacter crescentus''. These coiled-coil peptides (K-coil: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189012">BBa_K1189012</a> , E-coil: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189013">BBa_K1189013</a>, K-coil-His: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189010">BBa_K1189010</a>, and E-coil-His: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189011">BBa_K1189011</a> )were constructed by iGEM Calgary 2013. In this model, Caulobacter A will contain chalcopyrite-binding peptides bound to chalcopyrite elusively while holding a K-coil interaction peptide. By addition of Caulobacter B which holds the E-coil interaction peptide, Caulobacter A will coalesce with Caulobacter B by E-coil and K-coil interactions, forming a greater mass that will selectively sink in a froth floation solution. Alternatively, we have considered floating bound chalcopyrite using gas vesicle proteins (a href="http://parts.igem.org/wiki/index.php/Part:BBa_I750016">BBa_I750016</a>) developed by iGEM Groningen 2009.  
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As a proof of concept, we have chosen to express these peptides to selectively bind to chalcopyrite, which will then fall out of solution by aggregation using two interacting coiled-coil peptides expressed on the S-layer of ''Caulobacter crescentus''. These coiled-coil peptides (K-coil: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189012">BBa_K1189012</a> , E-coil: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189013">BBa_K1189013</a>, K-coil-His: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189010">BBa_K1189010</a>, and E-coil-His: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189011">BBa_K1189011</a> )were constructed by iGEM Calgary 2013. In this model, Caulobacter A will contain chalcopyrite-binding peptides bound to chalcopyrite elusively while holding a K-coil interaction peptide. By addition of Caulobacter B which holds the E-coil interaction peptide, Caulobacter A will coalesce with Caulobacter B by E-coil and K-coil interactions, forming a greater mass that will selectively sink in a froth floation solution. Alternatively, we have considered floating bound chalcopyrite using gas vesicle proteins (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_I750016">BBa_I750016</a>) developed by iGEM Groningen 2009.  

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2014 UBC iGEM

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