Team:UFMG Brazil/Project/Notebook

From 2014.igem.org

(Difference between revisions)
 
Line 1: Line 1:
-
<html>
+
{{UFMG_Brazil/css}}
-
<style type="text/css">
+
<html class=" desktop landscape" lang="en">
-
.tg  {border-collapse:collapse;border-spacing:0;border-color:#ccc;border-width:1px;border-style:solid;}
+
 
-
.tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-color:#ccc;color:#333;background-color:#fff;}
+
<head>
-
.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-color:#ccc;color:#333;background-color:#f0f0f0;}
+
    <meta http-equiv="content-type" content="text/html; charset=UTF-8">
-
.tg .tg-qupy{font-weight:bold;background-color:#ffccc9}
+
    <title>Home UFMG Team</title>
-
.tg .tg-huad{background-color:#ffccc9}
+
    <meta charset="utf-8">
-
.tg .tg-4eph{background-color:#f9f9f9}
+
    <meta name="format-detection" content="telephone=no">
-
</style>
+
    <link rel="icon" href="images/favicon.ico" type="image/x-icon">
-
<table class="tg" style="undefined;table-layout: fixed; width: 758px">
+
    <link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/grid.css&action=raw&ctype=text/css">
-
<colgroup>
+
    <link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/style.css&action=raw&ctype=text/css">
-
<col style="width: 82.19999998807907px">
+
    <link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/font-awesome.css&action=raw&ctype=text/css" >   
-
<col style="width: 676.1999999880791px">
+
    <link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/owl.carousel.css&action=raw&ctype=text/css" >
-
</colgroup>
+
    <!--[if lt IE 9]>
-
   <tr>
+
        <div style=' clear: both; text-align:center; position: relative;'>
-
     <th class="tg-qupy">February</th>
+
            <a href="http://windows.microsoft.com/en-US/internet-explorer/products/ie/home?ocid=ie6_countdown_bannercode">
-
    <th class="tg-huad"> </th>
+
            <img src="http://storage.ie6countdown.com/assets/100/images/banners/warning_bar_0000_us.jpg" border="0" height="42" width="820" alt="You are using an outdated browser. For a faster, safer browsing experience, upgrade for free today." />
-
  </tr>
+
            </a>
-
  <tr>
+
        </div>
-
    <td class="tg-4eph">1st week</td>
+
        <script src="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/html5shiv.js&action=raw&ctype=text/javascript"></script>
-
    <td class="tg-4eph">*First meeting with the team</td>
+
        <link rel="stylesheet" type="text/css" media="screen" href="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/ie.css&action=raw&ctype=text/css">
-
  </tr>
+
    <![endif]-->
-
  <tr>
+
    <script>
-
    <td class="tg-031e">2nd week</td>
+
      document.getElementById('top-section').style.height = '10px';
-
    <td class="tg-031e"> </td>
+
      var left_menu = document.querySelectorAll(".left-menu");
-
  </tr>
+
      for (var i = 0, length = left_menu.length; i < length; i++) {
-
  <tr>
+
        left_menu[i].style.display = 'none';
-
    <td class="tg-4eph">3rd week</td>
+
      }
-
    <td class="tg-4eph"> </td>
+
      var right_menu = document.querySelectorAll(".right-menu");
-
  </tr>
+
      for (var i = 0, length = right_menu.length; i < length; i++) {
-
  <tr>
+
        right_menu[i].style.display = 'none';
-
    <td class="tg-031e">4th week</td>
+
      }
-
    <td class="tg-031e"> </td>
+
    </script>
-
  </tr>
+
    <style>
-
  <tr>
+
    .thumbnail {
-
    <td class="tg-4eph">5th week</td>
+
      position: relative;
-
    <td class="tg-4eph"> </td>
+
      overflow: hidden;
-
  </tr>
+
      text-align: center;
-
  <tr>
+
      padding: 0 0 30px;
-
    <td class="tg-qupy">March</td>
+
      border-bottom: 0px;
-
    <td class="tg-huad"> </td>
+
      margin-bottom: 0px;
-
  </tr>
+
      -webkit-transition: all 0.3s ease;
-
  <tr>
+
      transition: all 0.3s ease;
-
    <td class="tg-4eph">1st week</td>
+
    }
-
    <td class="tg-4eph"> </td>
+
    .thumbnail:after {
-
  </tr>
+
      position: absolute;
-
  <tr>
+
      content: "";
-
    <td class="tg-031e">2nd week</td>
+
      background: none;
-
    <td class="tg-031e"> </td>
+
      bottom: 0;
-
  </tr>
+
      left: 45%;
-
  <tr>
+
      height: 13px;
-
    <td class="tg-4eph">3rd week</td>
+
      width: 25px;
-
    <td class="tg-4eph">*Possibilities of themes: <br>    → Metabolic syndrome, <br>    → Promoter sensitive to sound <br>    → Use of yeast <br>    *Doodle <br>    *Description <br>    *Trying to get support from Pro-reitoria→ Professor. Adelina <br>    *Sponsorship</td>
+
      -webkit-transition: all 0.3s ease;
-
  </tr>
+
      transition: all 0.3s ease;
-
  <tr>
+
    }
-
    <td class="tg-031e">4th week</td>
+
    .thumbnail:hover:after {
-
    <td class="tg-031e">*Defined theme:<br>    →Use of yeast for detection and treatment of colon cancer</td>
+
      background: none;
-
  </tr>
+
    }
-
  <tr>
+
    .thumbnail:hover {
-
    <td class="tg-4eph">5th week</td>
+
      border-bottom: none;
-
    <td class="tg-4eph">*Vakinha (Crowd funding)</td>
+
    }
-
  </tr>
+
    .header {
-
  <tr>
+
      padding-bottom: 0px;
-
    <td class="tg-qupy">April</td>
+
    }
-
    <td class="tg-huad"> </td>
+
    .table td {
-
  </tr>
+
      font-family: 'Roboto', sans-serif;
-
  <tr>
+
      font-size: 14px;
-
    <td class="tg-4eph">1st week</td>
+
      padding: 0;
-
    <td class="tg-4eph">*Defined theme:<br>    →Use of yeast for detection and treatment of colon cancer</td>
+
      color: #eae6e3;
-
  </tr>
+
      border-collapse: separate;
-
  <tr>
+
      border-spacing: 2px;
-
    <td class="tg-031e">2nd week</td>
+
      border-style: solid;
-
    <td class="tg-031e">*Cancer of colon<br>    *Probiotic: UFMG 905 <br>    *Biomarkers: sensors <br>    *Human practices (Meagan)<br>    → Difficulties to do Science in Brazil<br>    →Documentary <br>    → Buttons "I'm from Latin America" <br>    → Lectures</td>
+
      border-width: 1px;
-
  </tr>
+
      overflow: hidden;
-
  <tr>
+
      word-break: normal;
-
    <td class="tg-4eph">3rd week</td>
+
      border: 1px solid #d9d6d3;
-
    <td class="tg-4eph">*Identification system Colon cancer (Carlos) <br>    →Quantify the DNA present in the feces of mice with cancer (Laila) <br>    →Kit for DNA extraction from faeces (Rodrigo); <br>    →Disclosure TV radio and medias <br>    →Refresh the iGEM Facebook page <br>    →Search for new options field of non-specific DNA binding</td>
+
      height: 48px;
-
  </tr>
+
      line-height: 18px;
-
  <tr>
+
    }
-
    <td class="tg-031e">4th week</td>
+
    .c1 {
-
    <td class="tg-031e">*Long strand DNA <br>    →Extracting DNA from faeces of mice<br>  <br>    →System <br>    *Chimera proteins: look at other<br>  teams <br>    * Importance of attendance at team<br>  meetings <br>    * Romario: looking press office<br>  <br>    *Oncologists <br>    →Situation of colon cancer <br>    →Diagnosis <br>    *CCSP-2 (Oncogenes 2005) → Von<br>  Willebrand <br>    →Search for markers <br>    →DNA (Carlos) <br>    →Carbohydrates (Laila / Isadora /<br>  Lucas) <br>    →CCSP-2 (Rodrigo and Michele) <br>    →Metabolites (Marcelo and Dani)<br>  <br>    *Need to find for sponsorship (Ciça<br>  and Chris) <br>    *Idea to elaborate questionnaire to<br>  other Latin American iGEM teams (Thiago and Veronica)</td>
+
      line-height: 48px !important;  
-
  </tr>
+
    }
-
  <tr>
+
    </style>
-
    <td class="tg-4eph">5th week</td>
+
</head>
-
    <td class="tg-4eph">*TALE: Specific Sequence. DNA binding <br>    *mCherry split: NEW !!! <br>    *Can we use FRET techniques? <br>    *mCherry modeling <br>    *Microsatellite <br>    *Synthesis of template DNA repeat<br>  <br>    *Ca-binding proteins overexpressed<br>  ++ <br>    *Biosafety <br>    *CCSP-2: two domains von wilebrand<br>  <br>    *Workers yeasts: central information<br>  (more than one signal <br>    "warning" the same cell)</td>
+
<body id="top">
-
  </tr>
+
 
-
  <tr>
+
  <!--========================================================
-
    <td class="tg-qupy">May</td>
+
                            HEADER
-
    <td class="tg-huad"> </td>
+
  =========================================================-->
-
  </tr>
+
 
-
  <tr>
+
<header id="header" class="header">
-
    <td class="tg-4eph">1st week</td>
+
    <div class="banner1">
-
    <td class="tg-4eph">*Division of the responsibilities:<br>    →Draw the biobricks (Carlos) <br>    →Collecting mice faeces (Laila) <br>    →Protocol for DNA extraction from faeces and quantification (Rodrigo and<br>  Michele) <br>    →Human Practices (Thiago Mafra) <br>    →Cancer in the past IGEM (Marcelo and Dani Chame) <br>    →Video animation of IGEM UFMG BRAZIL (Lucas and Isadora) <br>    →Sponsors (Cecilia, Chris, James and Veronica Bruno) <br>    →Translate questionnaire to Spanish (Dani Chame)</td>
+
      <div id="stuck_container">
-
  </tr>
+
          <nav>
-
  <tr>
+
              <div class="container">
-
    <td class="tg-031e">2nd week</td>
+
                  <div class="row">
-
    <td class="tg-031e">*Conversation with Paulo Sérgio Lacerda Beirão<br>    →Legislation of the country<br>    *Videos with testimonials about the science in the country of researchers</td>
+
                      <div class="grid_12">
-
  </tr>
+
                            <ul class="sf-menu">
-
  <tr>
+
                                <li><a href="https://2014.igem.org/Team:UFMG_Brazil">Home</a></li>
-
    <td class="tg-4eph">3rd week</td>
+
                                <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Team">Team</a></li>
-
    <td class="tg-4eph">*Metabolic Syndrome - network of diseases<br>    *Biomarkers choice → limit<br>    →Complexity<br>    →Physiology<br>    →Stability<br>    *Lenght DNA→ eletricity (3’<br>  5’)<br>    *Accumulation of signal<br>    *Transfer of fluorescence<br>    *DNA bound proteins: proteins<br>  crawling in the DNA<br>    *Noise/signal ratio: important for<br>  qualitative diagnosis<br>    *Stool: noise<br>    *Proximity can allow interaction<br>  with domains that will activate an enzyme that transform a smelly (banana?)<br>  substract<br>    OR<br>    *Smell constitutive?<br>    *Yeast die if it dos not meet<br>  signal.<br>    *Difference between short and long<br>  DNA<br>    *NEW idea: sound detection.<br>    *mCherry</td>
+
                                <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Safety">Safety</a></li>
-
  </tr>
+
                                <li class="current"><a href="#">Project</a>
-
  <tr>
+
                                    <ul style="display:none;">
-
    <td class="tg-031e">4th week</td>
+
                                        <li><a href="https://2014.igem.org/Team:UFMG Brazil/Project/Intro">Introduction</a></li>
-
    <td class="tg-031e">*Protocol for feces<br>    *Modeling (Gastone)<br>    →Probability of false positives <br>    *Yeast plasmids design <br>    *Paratope Fab / Histone <br>    *Validation in E.coli: pass after apra yeast synthesize:<br>    →TALE <br>    →mCherry <br>    →Long repetitive DNA <br>    →Short repetitive DNA</td>
+
                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Parts">Parts</a></li>
-
  </tr>
+
                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Protocols">Protocols and results</a></li>
-
  <tr>
+
                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Modelling">Modelling</a></li>
-
    <td class="tg-4eph">5th week</td>
+
                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Policy">Policy and practices</a></li>
-
    <td class="tg-4eph"> </td>
+
                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Notebook">Notebook</a></li>
-
  </tr>
+
                                    </ul>
-
  <tr>
+
                                </li>
-
    <td class="tg-qupy">June</td>
+
                                <li><a href="#">Thanks to</a>
-
    <td class="tg-huad"> </td>
+
                                    <ul style="display:none;">
-
  </tr>
+
                                      <li>
-
  <tr>
+
                                        <a href="https://2014.igem.org/Team:UFMG_Brazil/sponsors">Sponsors</a>
-
    <td class="tg-4eph">1st week</td>
+
                                      </li>
-
    <td class="tg-4eph">*Workshop Biosynth <br>    *How to earn money? <br>    →Brazilian companies? <br>    →INCT <br>    →Proreitoria <br>    →Mario Penna <br>    <br>    *Latin America Fan Page</td>
+
                                      <li>
-
  </tr>
+
                                        <a href="https://2014.igem.org/Team:UFMG_Brazil/attributions">Attributions</a>
-
  <tr>
+
                                      </li>
-
    <td class="tg-031e">2nd week</td>
+
                                    </ul>
-
    <td class="tg-031e">*RFC10 - Biobrick standard <br>    *Bsmb1 → TALE <br>    *Software Assembly? <br>    *Wiki <br>    *Wet lab - gene synthesis <br>    *How to earn money?</td>
+
                                </li>
-
  </tr>
+
                                <li>
-
  <tr>
+
                                  <div>
-
    <td class="tg-4eph">3rd week</td>
+
                                    <div style="padding-top:5px; display:inline-block;"><a href="https://igem.org/"><img id="igemmenulogo" src="https://static.igem.org/mediawiki/2014/6/60/Igemlogo_300px.png" alt=""></a></div>
-
    <td class="tg-4eph">*Discount in the synthesis <br>    *Enzymes: XbaI, SpeI, EcoR1, Pst1,<br>  AgeI, NgomIV, BsmbI <br>    →Phoneutria? <br>    *Jacques Nicoli→ Yeast <br>    *Biosafety (term 23/06) <br>    *Donation Fiocruz</td>
+
                                    <div style="padding-top:24px; display:inline-block;"><a href="#"><img id="topmenutoggle" src="https://static.igem.org/mediawiki/2014/5/5b/Onoff.png" alt="" style="cursor:pointer"></a></div>
-
  </tr>
+
                                  </div>
-
  <tr>
+
                                </li>
-
    <td class="tg-031e">4th week</td>
+
                            </ul>
-
    <td class="tg-031e">*New England Enzyme <br>    *Texts Wiki <br>    →Project <br>    →Team <br>    →Human practices <br>    →Facebook<br>    →Vakinha (Crowd funding)</td>
+
                        </div>
-
  </tr>
+
                  </div>
-
  <tr>
+
              </div>
-
    <td class="tg-4eph">5th week</td>
+
          </nav>
-
    <td class="tg-4eph"> </td>
+
      </div>
-
  </tr>
+
 
-
  <tr>
+
      <div class="container">
-
    <td class="tg-qupy">July</td>
+
          <div class="row">
-
    <td class="tg-huad"> </td>
+
              <div class="grid_12">
-
  </tr>
+
                    <div class="unit" style="padding-top:50px; padding-bottom: 20px;">
-
  <tr>
+
                      <div class="title1">- notebook -</div>
-
    <td class="tg-4eph">1st week</td>
+
                    </div>
-
    <td class="tg-4eph">* Experimental tests <br>    * shopping<br>    * Restriction enzymes <br>    * length of 110 nucleotides linker → → try a smaller <br>    *experimental <br>    →Biobrick assemble the plasmid <br>    →Sequencing<br>    →Express and perform the tests in vitro. <br>    →Write protocols for wiki <br>    *Modeling mathematics <br>    *Wiki: <br>    →Assemble layout and content. <br>    *Human Practices <br>    →Questionnaires <br>    →Sponsorships <br>    →Questions - students ufmg schools. <br>    *Bioethics</td>
+
                  <div class="slogan">See what we did in all this time!</div>
-
  </tr>
+
              </div>   
-
  <tr>
+
          </div>   
-
    <td class="tg-031e">2nd week</td>
+
      </div>
-
    <td class="tg-031e">*Price for synthesizing biobrick <br>    →Request assistance pro-reitoria <br>    <br>    *Modeling <br>    →test of interaction assemblies TALE + + Linker Hmcherry1 and 2 <br>    *Making a letter for sponsorship</td>
+
    </div>
-
  </tr>
+
</header>
-
  <tr>
+
<div class="clear"></div>
-
    <td class="tg-4eph">3rd week</td>
+
 
-
    <td class="tg-4eph">*Deadlines<br>    * Fundraising Ideas <br>    * Human Practices <br>    → Send e-mails with the questionnaires <br>    * Modeling of proteins <br>    *Extraction of faeces</td>
+
   <!--========================================================
-
  </tr>
+
                            CONTENT
-
  <tr>
+
  =========================================================-->
-
    <td class="tg-031e">4th week</td>
+
 
-
    <td class="tg-031e"> </td>
+
<div id="content">
-
  </tr>
+
     <div class="bg4 p8">
-
  <tr>
+
        <div class="container">
-
    <td class="tg-4eph">5th week</td>
+
            <div class="row">
-
    <td class="tg-4eph"> </td>
+
                <div class="grid_12">
-
  </tr>
+
                  <h2>Our weekly notebook</h2>
-
  <tr>
+
                 
-
    <td class="tg-qupy">August</td>
+
                  <p>In this section is described our weekly deeds for the development of the project!.<br>You can go directly to a month using the links above:</p>
-
    <td class="tg-huad"> </td>
+
                   
-
  </tr>
+
                    <div class="btns">
-
  <tr>
+
                      <a href="#feb" class="link" data-type="reset">February</a>
-
    <td class="tg-4eph">1st week</td>
+
                      <a href="#March" class="link" data-type="reset">March</a>
-
    <td class="tg-4eph">* Arrival of restriction enzyme <br>    → Work<br>    *Texts for wiki <br>    *inscription IGEM</td>
+
                      <a href="#April" class="link" data-type="reset">April</a>
-
  </tr>
+
                      <a href="#May" class="link" data-type="reset">May</a>
-
  <tr>
+
                      <a href="#June" class="link" data-type="reset">June</a>
-
    <td class="tg-031e">2nd week</td>
+
                      <a href="#July" class="link" data-type="reset">July</a>
-
    <td class="tg-031e">*Experiments</td>
+
                      <a href="#August" class="link" data-type="reset">August</a>
-
  </tr>
+
                      <a href="#September" class="link" data-type="reset">September</a>
-
  <tr>
+
                      <a href="#October" class="link" data-type="reset">October</a>
-
    <td class="tg-4eph">3rd week</td>
+
                      <a href="#November" class="link" data-type="reset">November</a>
-
    <td class="tg-4eph">*Sponsors</td>
+
                    </div>
-
  </tr>
+
 
-
  <tr>
+
                  <br>
-
    <td class="tg-031e">4th week</td>
+
                  <br>
-
    <td class="tg-031e">*Experiments<br>    * Human Practices <br>    →Results of the questionnaires<br>    * Modeling of proteins</td>
+
                 
-
  </tr>
+
                </div>
-
  <tr>
+
 
-
    <td class="tg-4eph">5th week</td>
+
                <div class="grid_12"> 
-
    <td class="tg-4eph"> </td>
+
                  <h3 style="color: #2e3e4b;" id="feb">February</h3>
-
  </tr>
+
                  <table class="table" style="margin: 15px 0 20px;">
-
  <tr>
+
                    <tr>
-
    <td class="tg-qupy">September</td>
+
                      <th>Week</th>
-
    <td class="tg-huad"> </td>
+
                      <th>Description</th>
-
  </tr>
+
                    </tr>
-
  <tr>
+
                    <tr>
-
    <td class="tg-4eph">1st week</td>
+
                      <td class="c1" style="width: 120px;">1 week</td>
-
    <td class="tg-4eph">*Meeting with Dr.Clélio Campolina, National minister of<br>  Science and Technology<br>    *Wiki</td>
+
                      <td style="">
-
  </tr>
+
                        <p style="padding-left:5px;">
-
  <tr>
+
                        *First meeting with the team
-
    <td class="tg-031e">2nd week</td>
+
                        </p>
-
    <td class="tg-031e">*Human Practices: low adhesion <br>    *Fetch data from investment in research <br>    *Participation in Latin America over the years in the iGem<br>    *Plot: X number of contacts feedback <br>    *Lack of appreciation of Science <br>    *Popular search</td>
+
                      </td>
-
  </tr>
+
                    </tr>
-
  <tr>
+
                    <tr>
-
    <td class="tg-4eph">3rd week</td>
+
                      <td class="c1" style="width: 120px;">2 week</td>
-
    <td class="tg-4eph"> </td>
+
                      <td style="">
-
  </tr>
+
                        <p style="padding-left:5px;">
-
  <tr>
+
                       
-
    <td class="tg-031e">4th week</td>
+
                        </p>
-
    <td class="tg-031e">*Experiments<br>    *Project Summary<br>    *Meeting with Dr. Evaldo Vilela,<br>  FAPEMIG director.</td>
+
                      </td>
-
  </tr>
+
                    </tr>
-
  <tr>
+
                    <tr>
-
    <td class="tg-4eph">5th week</td>
+
                      <td class="c1" style="width: 120px;">3 week</td>
-
    <td class="tg-4eph"> </td>
+
                      <td style="">
-
  </tr>
+
                        <p style="padding-left:5px;">
-
  <tr>
+
                       
-
    <td class="tg-qupy">October</td>
+
                        </p>
-
    <td class="tg-huad"> </td>
+
                      </td>
-
  </tr>
+
                    </tr>
-
  <tr>
+
                    <tr>
-
    <td class="tg-4eph">1st week</td>
+
                      <td class="c1" style="width: 120px;">4 week</td>
-
    <td class="tg-4eph">*Finish the Wiki <br>    Modeling <br>    *Experimental <br>    *Human practices. <br>    →Questionnaire in the 7 square</td>
+
                      <td style="">
-
  </tr>
+
                        <p style="padding-left:5px;">
-
  <tr>
+
                       
-
    <td class="tg-031e">2nd week</td>
+
                        </p>
-
    <td class="tg-031e">*Lecture to high school, Technical school and Graduation, at<br>  CEFET.<br>    * Survey: The Importance of Science <br>    * Concentrations DNA <br>    * Sponsors <br>    * Deadlines</td>
+
                      </td>
-
  </tr>
+
                    </tr>
-
  <tr>
+
                    <tr>
-
    <td class="tg-4eph">3rd week</td>
+
                      <td class="c1" style="width: 120px;">5 week</td>
-
    <td class="tg-4eph">* Experiments <br>    →Results <br>    * Human Practices <br>    →Results <br>    *Modeling of proteins<br>    →Results <br>    * Modeling mathematics <br>    →Results <br>    * Sponsors <br>    →Tshirt <br>    →Who helped?</td>
+
                      <td style="">
-
  </tr>
+
                        <p style="padding:5px;">
-
  <tr>
+
                       
-
    <td class="tg-031e">4th week</td>
+
                        </p>
-
    <td class="tg-031e"> </td>
+
                      </td>
-
  </tr>
+
                    </tr>                                                                                      
-
  <tr>
+
                  </table>                
-
    <td class="tg-4eph">5th week</td>
+
                  <br>
-
    <td class="tg-4eph"> </td>
+
 
-
  </tr>
+
                  <h3 style="color: #2e3e4b;" id="March">March</h3>
-
  <tr>
+
                  <table class="table" style="margin: 15px 0 20px;">
-
    <td class="tg-qupy">November</td>
+
                    <tr>
-
    <td class="tg-huad"> </td>
+
                      <th>Week</th>
-
  </tr>
+
                      <th>Description</th>
-
  <tr>
+
                    </tr>
-
    <td class="tg-4eph"></td>
+
                    <tr>
-
    <td class="tg-4eph">World Championship Jamboree.</td>
+
                      <td class="c1" style="width: 120px;">1 week</td>
-
  </tr>
+
                      <td style="">
-
</table>
+
                        <p style="padding-left:5px;">
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                       
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Possibilities of themes: <br>    → Metabolic syndrome, <br>    → Promoter sensitive to sound <br>    → Use of yeast <br>    *Doodle <br>    *Description <br>    *Trying to get support from Pro-reitoria→ Professor. Adelina <br>    *Sponsorship
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Defined theme:<br>    →Use of yeast for detection and treatment of colon cancer
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;">
 +
                        *Vakinha (Crowd funding)
 +
                        </p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="April" >April</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Defined theme:<br>    →Use of yeast for detection and treatment of colon cancer
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Cancer of colon<br>    *Probiotic: UFMG 905 <br>    *Biomarkers: sensors <br>    *Human practices (Meagan)<br>    → Difficulties to do Science in Brazil<br>    →Documentary <br>    → Buttons "I'm from Latin America" <br>    → Lectures
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Identification system Colon cancer (Carlos) <br>    →Quantify the DNA present in the feces of mice with cancer (Laila) <br>    →Kit for DNA extraction from faeces (Rodrigo); <br>    →Disclosure TV radio and medias <br>    →Refresh the iGEM Facebook page <br>    →Search for new options field of non-specific DNA binding
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Long strand DNA <br>    →Extracting DNA from faeces of mice<br>  <br>    →System <br>    *Chimera proteins: look at other<br>  teams <br>    * Importance of attendance at team<br>  meetings <br>    * Romario: looking press office<br>  <br>    *Oncologists <br>    →Situation of colon cancer <br>    →Diagnosis <br>    *CCSP-2 (Oncogenes 2005) → Von<br>  Willebrand <br>    →Search for markers <br>    →DNA (Carlos) <br>    →Carbohydrates (Laila / Isadora /<br>  Lucas) <br>    →CCSP-2 (Rodrigo and Michele) <br>    →Metabolites (Marcelo and Dani)<br>  <br>    *Need to find for sponsorship (Ciça<br>  and Chris) <br>    *Idea to elaborate questionnaire to<br>  other Latin American iGEM teams (Thiago and Veronica)
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;">
 +
                        *TALE: Specific Sequence. DNA binding <br>    *mCherry split: NEW !!! <br>    *Can we use FRET techniques? <br>    *mCherry modeling <br>    *Microsatellite <br>    *Synthesis of template DNA repeat<br>  <br>    *Ca-binding proteins overexpressed<br>  ++ <br>    *Biosafety <br>    *CCSP-2: two domains von wilebrand<br>  <br>    *Workers yeasts: central information<br>  (more than one signal <br>    "warning" the same cell)
 +
                        </p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="May" >May</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Division of the responsibilities:<br>    →Draw the biobricks (Carlos) <br>    →Collecting mice faeces (Laila) <br>    →Protocol for DNA extraction from faeces and quantification (Rodrigo and<br>  Michele) <br>    →Human Practices (Thiago Mafra) <br>    →Cancer in the past IGEM (Marcelo and Dani Chame) <br>    →Video animation of IGEM UFMG BRAZIL (Lucas and Isadora) <br>    →Sponsors (Cecilia, Chris, James and Veronica Bruno) <br>    →Translate questionnaire to Spanish (Dani Chame)
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Conversation with Paulo Sérgio Lacerda Beirão<br>    →Legislation of the country<br>    *Videos with testimonials about the science in the country of researchers
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Metabolic Syndrome - network of diseases<br>    *Biomarkers choice → limit<br>    →Complexity<br>    →Physiology<br>    →Stability<br>    *Lenght DNA→ eletricity (3’<br>  5’)<br>    *Accumulation of signal<br>    *Transfer of fluorescence<br>    *DNA bound proteins: proteins<br>  crawling in the DNA<br>    *Noise/signal ratio: important for<br>  qualitative diagnosis<br>    *Stool: noise<br>    *Proximity can allow interaction<br>  with domains that will activate an enzyme that transform a smelly (banana?)<br>  substract<br>    OR<br>    *Smell constitutive?<br>    *Yeast die if it dos not meet<br>  signal.<br>    *Difference between short and long<br>  DNA<br>    *NEW idea: sound detection.<br>    *mCherry
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Protocol for feces<br>    *Modeling (Gastone)<br>    →Probability of false positives <br>    *Yeast plasmids design <br>    *Paratope Fab / Histone <br>    *Validation in E.coli: pass after apra yeast synthesize:<br>    →TALE <br>    →mCherry <br>    →Long repetitive DNA <br>    →Short repetitive DNA
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;">
 +
                        *TALE: Specific Sequence. DNA binding <br>    *mCherry split: NEW !!! <br>    *Can we use FRET techniques? <br>    *mCherry modeling <br>    *Microsatellite <br>    *Synthesis of template DNA repeat<br>  <br>    *Ca-binding proteins overexpressed<br>  ++ <br>    *Biosafety <br>    *CCSP-2: two domains von wilebrand<br>  <br>    *Workers yeasts: central information<br>  (more than one signal <br>    "warning" the same cell)
 +
                        </p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="June">June</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Workshop Biosynth <br>    *How to earn money? <br>    →Brazilian companies? <br>    →INCT <br>    →Proreitoria <br>    →Mario Penna <br>    <br>    *Latin America Fan Page
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *RFC10 - Biobrick standard <br>    *Bsmb1 → TALE <br>    *Software Assembly? <br>    *Wiki <br>    *Wet lab - gene synthesis <br>    *How to earn money?
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Discount in the synthesis <br>    *Enzymes: XbaI, SpeI, EcoR1, Pst1,<br>  AgeI, NgomIV, BsmbI <br>    →Phoneutria? <br>    *Jacques Nicoli→ Yeast <br>    *Biosafety (term 23/06) <br>    *Donation Fiocruz
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *New England Enzyme <br>    *Texts Wiki <br>    →Project <br>    →Team <br>    →Human practices <br>    →Facebook<br>    →Vakinha (Crowd funding)
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;"></p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="July">July</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        * Experimental tests <br>    * shopping<br>    * Restriction enzymes <br>    * length of 110 nucleotides linker → → try a smaller <br>    *experimental <br>    →Biobrick assemble the plasmid <br>    →Sequencing<br>    →Express and perform the tests in vitro. <br>    →Write protocols for wiki <br>    *Modeling mathematics <br>    *Wiki: <br>    →Assemble layout and content. <br>    *Human Practices <br>    →Questionnaires <br>    →Sponsorships <br>    →Questions - students ufmg schools. <br>    *Bioethics
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
*Price for synthesizing biobrick <br>    →Request assistance pro-reitoria <br>    <br>    *Modeling <br>    →test of interaction assemblies TALE + + Linker Hmcherry1 and 2 <br>    *Making a letter for sponsorship
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Deadlines<br>    * Fundraising Ideas <br>    * Human Practices <br>    → Send e-mails with the questionnaires <br>    * Modeling of proteins <br>    *Extraction of faeces
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                       
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;"></p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="August">August</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        * Arrival of restriction enzyme <br>    → Work<br>    *Texts for wiki <br>    *inscription IGEM
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                          *Experiments
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Sponsors
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Experiments<br>    * Human Practices <br>    →Results of the questionnaires<br>    * Modeling of proteins
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;"></p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="September">September</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Meeting with Dr.Clélio Campolina, National minister of<br>  Science and Technology<br>    *Wiki
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                          *Human Practices: low adhesion <br>    *Fetch data from investment in research <br>    *Participation in Latin America over the years in the iGem<br>    *Plot: X number of contacts feedback <br>    *Lack of appreciation of Science <br>    *Popular search
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                       
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Experiments<br>    *Project Summary<br>    *Meeting with Dr. Evaldo Vilela,<br>  FAPEMIG director.
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;"></p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="October">October</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        *Finish the Wiki <br>    Modeling <br>    *Experimental <br>    *Human practices. <br>    →Questionnaire in the 7 square
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">2 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                          *Lecture to high school, Technical school and Graduation, at<br>  CEFET.<br>    * Survey: The Importance of Science <br>    * Concentrations DNA <br>    * Sponsors <br>    * Deadlines
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">3 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        * Experiments <br>    →Results <br>    * Human Practices <br>    →Results <br>    *Modeling of proteins<br>    →Results <br>    * Modeling mathematics <br>    →Results <br>    * Sponsors <br>    →Tshirt <br>    →Who helped?
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">4 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        </p>
 +
                      </td>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">5 week</td>
 +
                      <td style="">
 +
                        <p style="padding:5px;"></p>
 +
                      </td>
 +
                    </tr>                                                                                      
 +
                  </table>                
 +
                  <br>
 +
 
 +
                  <h3 style="color: #2e3e4b;" id="November">November</h3>
 +
                  <table class="table" style="margin: 15px 0 20px;">
 +
                    <tr>
 +
                      <th>Week</th>
 +
                      <th>Description</th>
 +
                    </tr>
 +
                    <tr>
 +
                      <td class="c1" style="width: 120px;">1 week</td>
 +
                      <td style="">
 +
                        <p style="padding-left:5px;">
 +
                        World Championship Jamboree.
 +
                        </p>
 +
                      </td>
 +
                    </tr>                                                                                    
 +
                  </table>                
 +
                  <br>
 +
 
 +
                </div>
 +
            </div>           
 +
        </div>
 +
    </div>
 +
</div>
 +
 
 +
  <!--========================================================
 +
                            FOOTER
 +
  =========================================================-->
 +
 
 +
<footer id="footer">
 +
      <div class="container">
 +
          <div class="row">
 +
              <div class="grid_12">
 +
                  <a class="logo1" href="https://2014.igem.org/Team:UFMG_Brazil">
 +
                    <img src="https://static.igem.org/mediawiki/2014/8/84/Yeastlogo_small_gray.png" alt="">
 +
                  </a>
 +
                  <div class="copy">
 +
                      <span>&nbsp;&copy;</span>
 +
                      <span id="copyright-year"></span>
 +
                      <span>|</span>
 +
                      <span><a href="https://2014.igem.org/Team:UFMG_Brazil">Team UFMG Brazil</a></span>
 +
                  </div>
 +
              </div>     
 +
          </div>
 +
      </div> 
 +
  </footer>
 +
 
 +
    <script src="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/jquery.js&action=raw&ctype=text/javascript"></script>
 +
    <script src="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/jquery-migrate-1.2.1.js&action=raw&ctype=text/javascript
 +
"></script>
 +
    <script src="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/owl.carousel.js&action=raw&ctype=text/javascript
 +
"></script>     
 +
    <script src="https://2014.igem.org/wiki/index.php?title=Template:UFMG_Brazil/script.js&action=raw&ctype=text/javascript
 +
"></script>
 +
 
 +
    <meta name="viewport" content="width=device-width,initial-scale=1.0,user-scalable=0">
 +
    <script>
 +
        $(document).ready(function() {
 +
            $("#owl").owlCarousel({
 +
                items: 4,
 +
                itemsDesktop: [1299, 4],
 +
                itemsTablet: [995, 3],
 +
                itemsTabletSmall: [767, 1],
 +
                itemsMobile: [479, 1],
 +
                lazyLoad: true,
 +
                pagination: false,
 +
                navigation: true
 +
            });
 +
        });
 +
        $( document ).ready(function() {
 +
          $('#topmenutoggle').click(function(){
 +
            toggleMenu();
 +
          });
 +
        });
 +
        function toggleMenu(){
 +
          if($('#top-section').css("height") == "25px"){
 +
              $('#top-section').css("height", "10px");
 +
              $('.left-menu').hide();
 +
              $('.right-menu').hide();
 +
          }
 +
          else{
 +
              $('#top-section').css("height", "25px");
 +
              $('.left-menu').show();
 +
              $('.right-menu').show();
 +
          }
 +
        }
 +
    </script>
 +
 
 +
    <a style="margin-right: -49px; right: 50%; display: none;" href="#" id="toTop" class="fa fa-chevron-up"></a>
 +
</body>
 +
 
</html>
</html>

Latest revision as of 02:51, 18 October 2014

Home UFMG Team

Our weekly notebook

In this section is described our weekly deeds for the development of the project!.
You can go directly to a month using the links above:



February

Week Description
1 week

*First meeting with the team

2 week

3 week

4 week

5 week


March

Week Description
1 week

2 week

3 week

*Possibilities of themes:
→ Metabolic syndrome,
→ Promoter sensitive to sound
→ Use of yeast
*Doodle
*Description
*Trying to get support from Pro-reitoria→ Professor. Adelina
*Sponsorship

4 week

*Defined theme:
→Use of yeast for detection and treatment of colon cancer

5 week

*Vakinha (Crowd funding)


April

Week Description
1 week

*Defined theme:
→Use of yeast for detection and treatment of colon cancer

2 week

*Cancer of colon
*Probiotic: UFMG 905
*Biomarkers: sensors
*Human practices (Meagan)
→ Difficulties to do Science in Brazil
→Documentary
→ Buttons "I'm from Latin America"
→ Lectures

3 week

*Identification system Colon cancer (Carlos)
→Quantify the DNA present in the feces of mice with cancer (Laila)
→Kit for DNA extraction from faeces (Rodrigo);
→Disclosure TV radio and medias
→Refresh the iGEM Facebook page
→Search for new options field of non-specific DNA binding

4 week

*Long strand DNA
→Extracting DNA from faeces of mice

→System
*Chimera proteins: look at other
teams
* Importance of attendance at team
meetings
* Romario: looking press office

*Oncologists
→Situation of colon cancer
→Diagnosis
*CCSP-2 (Oncogenes 2005) → Von
Willebrand
→Search for markers
→DNA (Carlos)
→Carbohydrates (Laila / Isadora /
Lucas)
→CCSP-2 (Rodrigo and Michele)
→Metabolites (Marcelo and Dani)

*Need to find for sponsorship (Ciça
and Chris)
*Idea to elaborate questionnaire to
other Latin American iGEM teams (Thiago and Veronica)

5 week

*TALE: Specific Sequence. DNA binding
*mCherry split: NEW !!!
*Can we use FRET techniques?
*mCherry modeling
*Microsatellite
*Synthesis of template DNA repeat

*Ca-binding proteins overexpressed
++
*Biosafety
*CCSP-2: two domains von wilebrand

*Workers yeasts: central information
(more than one signal
"warning" the same cell)


May

Week Description
1 week

*Division of the responsibilities:
→Draw the biobricks (Carlos)
→Collecting mice faeces (Laila)
→Protocol for DNA extraction from faeces and quantification (Rodrigo and
Michele)
→Human Practices (Thiago Mafra)
→Cancer in the past IGEM (Marcelo and Dani Chame)
→Video animation of IGEM UFMG BRAZIL (Lucas and Isadora)
→Sponsors (Cecilia, Chris, James and Veronica Bruno)
→Translate questionnaire to Spanish (Dani Chame)

2 week

*Conversation with Paulo Sérgio Lacerda Beirão
→Legislation of the country
*Videos with testimonials about the science in the country of researchers

3 week

*Metabolic Syndrome - network of diseases
*Biomarkers choice → limit
→Complexity
→Physiology
→Stability
*Lenght DNA→ eletricity (3’
5’)
*Accumulation of signal
*Transfer of fluorescence
*DNA bound proteins: proteins
crawling in the DNA
*Noise/signal ratio: important for
qualitative diagnosis
*Stool: noise
*Proximity can allow interaction
with domains that will activate an enzyme that transform a smelly (banana?)
substract
OR
*Smell constitutive?
*Yeast die if it dos not meet
signal.
*Difference between short and long
DNA
*NEW idea: sound detection.
*mCherry

4 week

*Protocol for feces
*Modeling (Gastone)
→Probability of false positives
*Yeast plasmids design
*Paratope Fab / Histone
*Validation in E.coli: pass after apra yeast synthesize:
→TALE
→mCherry
→Long repetitive DNA
→Short repetitive DNA

5 week

*TALE: Specific Sequence. DNA binding
*mCherry split: NEW !!!
*Can we use FRET techniques?
*mCherry modeling
*Microsatellite
*Synthesis of template DNA repeat

*Ca-binding proteins overexpressed
++
*Biosafety
*CCSP-2: two domains von wilebrand

*Workers yeasts: central information
(more than one signal
"warning" the same cell)


June

Week Description
1 week

*Workshop Biosynth
*How to earn money?
→Brazilian companies?
→INCT
→Proreitoria
→Mario Penna

*Latin America Fan Page

2 week

*RFC10 - Biobrick standard
*Bsmb1 → TALE
*Software Assembly?
*Wiki
*Wet lab - gene synthesis
*How to earn money?

3 week

*Discount in the synthesis
*Enzymes: XbaI, SpeI, EcoR1, Pst1,
AgeI, NgomIV, BsmbI
→Phoneutria?
*Jacques Nicoli→ Yeast
*Biosafety (term 23/06)
*Donation Fiocruz

4 week

*New England Enzyme
*Texts Wiki
→Project
→Team
→Human practices
→Facebook
→Vakinha (Crowd funding)

5 week


July

Week Description
1 week

* Experimental tests
* shopping
* Restriction enzymes
* length of 110 nucleotides linker → → try a smaller
*experimental
→Biobrick assemble the plasmid
→Sequencing
→Express and perform the tests in vitro.
→Write protocols for wiki
*Modeling mathematics
*Wiki:
→Assemble layout and content.
*Human Practices
→Questionnaires
→Sponsorships
→Questions - students ufmg schools.
*Bioethics

2 week

*Price for synthesizing biobrick
→Request assistance pro-reitoria

*Modeling
→test of interaction assemblies TALE + + Linker Hmcherry1 and 2
*Making a letter for sponsorship

3 week

*Deadlines
* Fundraising Ideas
* Human Practices
→ Send e-mails with the questionnaires
* Modeling of proteins
*Extraction of faeces

4 week

5 week


August

Week Description
1 week

* Arrival of restriction enzyme
→ Work
*Texts for wiki
*inscription IGEM

2 week

*Experiments

3 week

*Sponsors

4 week

*Experiments
* Human Practices
→Results of the questionnaires
* Modeling of proteins

5 week


September

Week Description
1 week

*Meeting with Dr.Clélio Campolina, National minister of
Science and Technology
*Wiki

2 week

*Human Practices: low adhesion
*Fetch data from investment in research
*Participation in Latin America over the years in the iGem
*Plot: X number of contacts feedback
*Lack of appreciation of Science
*Popular search

3 week

4 week

*Experiments
*Project Summary
*Meeting with Dr. Evaldo Vilela,
FAPEMIG director.

5 week


October

Week Description
1 week

*Finish the Wiki
Modeling
*Experimental
*Human practices.
→Questionnaire in the 7 square

2 week

*Lecture to high school, Technical school and Graduation, at
CEFET.
* Survey: The Importance of Science
* Concentrations DNA
* Sponsors
* Deadlines

3 week

* Experiments
→Results
* Human Practices
→Results
*Modeling of proteins
→Results
* Modeling mathematics
→Results
* Sponsors
→Tshirt
→Who helped?

4 week

5 week


November

Week Description
1 week

World Championship Jamboree.