Team:Evry/Notebook/Sensing/PCBs/09-08-2014

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Revision as of 02:50, 18 October 2014

Picture

Sensor construction bphR2/PbpR1

A new tentative of golden gate has been done with modification of volumes of each part:

  • J23114-rbs : 0,12µL
  • bphR2: 1,05µl
  • bphR1:0,31µl
  • RFP : 0,64µl
  • Terminator: 0,67µl
  • pSB1C3,G3: 1µl
    The mix enzyme was inchanged and the program is of 8h Sep 08

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