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Revision as of 02:38, 18 October 2014

Cloning

The protocols for our methods can be found here.

During our experiments, we conducted a lot of PCRs with different Primers. In order to stay on top of things, we numbered Primers, PCRs and constructs. You can download both lists with detailed information about Primer composition and about PCRs in the following documents:

p14rz_002

PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
Digest: pKM006, EcoRI + NotI; PCR-product
Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7

p14rz_003

PCR 3
Digest 3, 4
Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8

p14rz_004

PCR 21,22
Digest -
Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2

p14rz_005

PCR 4,5
Digest 5
Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3

p14rz_006

PCR 17,18
Digest 5
Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4

p14rz_007

PCR 37,38
Digest 21
Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7

p14rz_008

PCR blue light receptor; Template: pQXCIN-SLC7A1
Digest pKM006 EcoRI, NotI
Testdigest with SmaI in Cutsmart, expected bands: 5,7

p14rz_009

PCR 39,40
Digest 5
Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7

p14rz_010

PCR 23,24
Digest 12
Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56

p14ls_003

PCR 7,9,10
Digest -
Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4

p14vv_001

PCR Primers vv003,vv004; Template: pMIG
Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6

p14vv_002

PCR -
Digest 8,11
Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4

p14vv_003

PCR pMIG with primer vv003+vv004-
Digest xx
Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5

p14vv_006

PCR 26
Digest 13
Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6

p14vv_007

PCR 27
Digest 14
Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66

p14vv_008

PCR 43
Digest 22
Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75

p14vv_009

PCR 44
Digest 22
Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75

p14vv_010

PCR 45
Digest 22
Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2

p14vv_012

PCR 46
Digest 22
Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4

p14vv_013

PCR 47,48,49
Digest 22
Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2

p14vv_014

PCR 50,51
Digest 22
Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66

p14zl_010

PCR 1,2
Digest 1
Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1

p14zl_011

PCR 19,20
Digest 1
Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43

p14zl_016

PCR 52,53
Digest 22
Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7

p14zl_017

PCR 64,65
Digest 24
testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9

p14zl_020

PCR 59,60
Digest 24
testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8

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 <p>During our experiments, we conducted a lot of PCRs with different Primers. In order to stay on top of things, we numbered Primers, PCRs and constructs. You can download both lists with detailed information about Primer composition and about PCRs in the following documents:</p>
+<section>
-	<figure>
+<div class="row category-row">
+      <div class="col-sm-6">
+ 	<figure>
          <img src="https://static.igem.org/mediawiki/2014/a/a6/2014Freiburg_Labbook_Digests.JPG">
-     </figure>
+     </figure></div>
+<div class="col-sm-6">
+</div>
+</div>
+</section>

Team:Freiburg/Content/Notebook