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Team:Bordeaux/NotebookAugust26
From 2014.igem.org
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Digestion pSB1C3 | Digestion pSB1C3 | ||
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Miniprep pSB1C3<br> | Miniprep pSB1C3<br> | ||
Revision as of 02:29, 18 October 2014
26th August
Molecular biology
-
pSB6A1 : 193 CFU
ELP-ELP 1: 1776 CFU
ELP-ELP 2: 1104 CFU
ELP-SLP: 2016 CFU
ELP-SLP: 2400 CFU
ELP-RLP: 1876 CFU
ELP-RLP: 2368 CFU
SLP-SLP: 93 CFU
SLP-SLP: 91 CFU
SLP-RLP: 10 CFU
SLP-RLP: 248 CFU
RLP-RLP: 1872 CFU
RLP-RLP: 1424 CFU
-
Digestion pSB1C3
Buffer (cutsmart) 5μl Xba1 0,5μl Spe1 0,5μl DNA 10μl H2O qsp 50μl
=> 1h at 37°C and gel 1% (30min, 100V) -
Miniprep pSB1C3
Sample 1: 224,2ng/μl
Sample 2: 313,4ng/μl<
Sample 3: 269,9ng/μl
