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From 2014.igem.org

Revision as of 02:15, 18 October 2014 (view source) Jr (Talk | contribs) ← Older edit		Latest revision as of 02:25, 18 October 2014 (view source) Jr (Talk | contribs)
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	<ul class="day-detail">		<ul class="day-detail">
-	<li>Gene SLP synthesis<br><br>	+	<li>SLP gene synthesis<br><br>
	PCR step 3 : Vtot = 50µL<br>		PCR step 3 : Vtot = 50µL<br>
	<br>		<br>

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Latest revision as of 02:25, 18 October 2014

15^th July

• SLP gene synthesis
  
  PCR step 3 : Vtot = 50µL
  
  10µL phusion buffer 5X
  1µL dNTP 10mM
  0,5µL phusion polymérase
  2,5µL DMSO
  2,5µL Primer 1 à 10mM
  2,5µL Primer 8 à 10mM
  10µL 3° ou 3°°
  21µL H2O
  
  PCR Parameter:
  98°C 5 min
  35 cycles : 98°C 10s, 69°C 20s, 72°C 1min
  Final extension 72°C 10min
  End at 4°C
  
  
  
  
  
  

Please look our planning of production and purification