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Team:BYU Provo/Notebook/Metabolism/febapr
From 2014.igem.org
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<h3>March 21, 2014</h3> | <h3>March 21, 2014</h3> | ||
<p>Searched articles on the effectiveness of macrolide and beta-lactam degradation enzymes. Researched bacteria with a known gene sequences to degrade both types of antibiotics.</p> | <p>Searched articles on the effectiveness of macrolide and beta-lactam degradation enzymes. Researched bacteria with a known gene sequences to degrade both types of antibiotics.</p> | ||
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| + | <h2>Week of March 29th, 2014</h2> | ||
| + | <h3>March 24, 2014</h3> | ||
| + | <p>Investigated macrolide antibiotic degradation, settling on the ethryomycin esterase as the enzyme. Found part BBa_K1159000 in the IGEM registry which contains the Erythromycin Esterase Type II (EreB) gene that degrades macrolides.</p> | ||
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| + | <h3>March 28, 2014</h3> | ||
| + | <p>Used the Anderson Promoter Collection to determine which promoters have the highest rate of expression. Antibiotic degradation genes would need medium to strong expression to be useful to the bacteria</p> | ||
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</html> | </html> | ||
Revision as of 20:42, 18 July 2014
BYU 2014 Notebook |
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Week of March 22nd
March 17, 2014
Researched articles on the effects of heavy metals on waste water treatment plants and the effects of heavy metals on bacteria to prepare for our presentation on N.multiformis metabolism optimization. Searched for common bacteria with heavy metal resistance genes that could be possibly insert into our bacterial chassis; finding sequences and reading about success rates in data of those that had been transferred.
March 20, 2014
Searched for the most commonly prescribed antibiotics in the United States. Top prescribed antibiotics include penicillins and macrolides according to the New England Journal of Medicine (2013) [http://www.nejm.org/doi/full/10.1056/NEJMc1212055#t=article]
March 21, 2014
Searched articles on the effectiveness of macrolide and beta-lactam degradation enzymes. Researched bacteria with a known gene sequences to degrade both types of antibiotics.
Week of March 29th, 2014
March 24, 2014
Investigated macrolide antibiotic degradation, settling on the ethryomycin esterase as the enzyme. Found part BBa_K1159000 in the IGEM registry which contains the Erythromycin Esterase Type II (EreB) gene that degrades macrolides.
March 28, 2014
Used the Anderson Promoter Collection to determine which promoters have the highest rate of expression. Antibiotic degradation genes would need medium to strong expression to be useful to the bacteria
