Parts

• BBa_K1559000 tracr RNA to be used with Cas9
• BBa_K1559001 Cas9 and crRNA array
• BBa_K1559002 rearranged CRISPR/Cas9 system without promoter
• BBa_K1559003 CRISPR/Cas9 system with Anderson high-expression constitutive promoter
• BBa_K1559004 CRISPR/Cas9 system with Anderson medium-expression constitutive promoter
• BBa_K1559005 CRISPR/Cas9 system with Anderson low-expression constitutive promoter
• BBa_K1559006 CRISPR/Cas9 system with pBAD inducible promoter
• BBa_K1559007 CRISPR/Cas9 system with pLac inducible promoter
• BBa_K1559008 CRISPR/Cas9 system with pRha inducible promoter
• BBa_K1559009 Randomized protospacer for CRISPR/Cas9 System
• BBa_K1559010 RFP-Targeting protospacer for CRISPR/Cas9 system
• BBa_K1559011 Low-copy plasmid pTRC99A (trc promoter and lacI removed)

Our assembly strategy involved assembling 4 fragments together (see design page). Over the course of the project, we made these 4 fragments but were unable to assemble them together into one plasmid. We managed to biobrick one of these fragments, the tracrRNA fragment (BBa_K1559000) before the parts submission deadline.

We have also documented and added the sequences of the rest of the parts we designed to the parts registry in the hopes that future iGEM teams may find them useful.