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From 2014.igem.org

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Revision as of 02:09, 18 October 2014

February
1º week	*First meeting with the team
2º week
3º week
4º week
5º week
March
1º week
2º week
3º week	*Possibilities of themes: → Metabolic syndrome, → Promoter sensitive to sound → Use of yeast *Doodle *Description *Trying to get support from Pro-reitoria→ Professor. Adelina *Sponsorship
4º week	*Defined theme: →Use of yeast for detection and treatment of colon cancer
5º week	*Vakinha (Crowd funding)
April
1º week	*Defined theme: →Use of yeast for detection and treatment of colon cancer
2º week	*Cancer of colon *Probiotic: UFMG 905 *Biomarkers: sensors *Human practices (Meagan) → Difficulties to do Science in Brazil →Documentary → Buttons "I'm from Latin America" → Lectures
3º week	*Identification system Colon cancer (Carlos) →Quantify the DNA present in the feces of mice with cancer (Laila) →Kit for DNA extraction from faeces (Rodrigo); →Disclosure TV radio and medias →Refresh the iGEM Facebook page →Search for new options field of non-specific DNA binding
4º week	*Long strand DNA →Extracting DNA from faeces of mice →System *Chimera proteins: look at other teams * Importance of attendance at team meetings * Romario: looking press office *Oncologists →Situation of colon cancer →Diagnosis *CCSP-2 (Oncogenes 2005) → Von Willebrand →Search for markers →DNA (Carlos) →Carbohydrates (Laila / Isadora / Lucas) →CCSP-2 (Rodrigo and Michele) →Metabolites (Marcelo and Dani) *Need to find for sponsorship (Ciça and Chris) *Idea to elaborate questionnaire to other Latin American iGEM teams (Thiago and Veronica)
5º week	*TALE: Specific Sequence. DNA binding *mCherry split: NEW !!! *Can we use FRET techniques? *mCherry modeling *Microsatellite *Synthesis of template DNA repeat *Ca-binding proteins overexpressed ++ *Biosafety *CCSP-2: two domains von wilebrand *Workers yeasts: central information (more than one signal "warning" the same cell)
May
1º week	*Division of the responsibilities: →Draw the biobricks (Carlos) →Collecting mice faeces (Laila) →Protocol for DNA extraction from faeces and quantification (Rodrigo and Michele) →Human Practices (Thiago Mafra) →Cancer in the past IGEM (Marcelo and Dani Chame) →Video animation of IGEM UFMG BRAZIL (Lucas and Isadora) →Sponsors (Cecilia, Chris, James and Veronica Bruno) →Translate questionnaire to Spanish (Dani Chame)
2º week	*Conversation with Paulo Sérgio Lacerda Beirão →Legislation of the country *Videos with testimonials about the science in the country of researchers
3º week	*Metabolic Syndrome - network of diseases *Biomarkers choice → limit →Complexity →Physiology →Stability *Lenght DNA→ eletricity (3’ 5’) *Accumulation of signal *Transfer of fluorescence *DNA bound proteins: proteins crawling in the DNA *Noise/signal ratio: important for qualitative diagnosis *Stool: noise *Proximity can allow interaction with domains that will activate an enzyme that transform a smelly (banana?) substract OR *Smell constitutive? *Yeast die if it dos not meet signal. *Difference between short and long DNA *NEW idea: sound detection. *mCherry
4º week	*Protocol for feces *Modeling (Gastone) →Probability of false positives *Yeast plasmids design *Paratope Fab / Histone *Validation in E.coli: pass after apra yeast synthesize: →TALE →mCherry →Long repetitive DNA →Short repetitive DNA
5º week
June
1º week	*Workshop Biosynth *How to earn money? →Brazilian companies? →INCT →Proreitoria →Mario Penna *Latin America Fan Page
2º week	*RFC10 - Biobrick standard *Bsmb1 → TALE *Software Assembly? *Wiki *Wet lab - gene synthesis *How to earn money?
3º week	*Discount in the synthesis *Enzymes: XbaI, SpeI, EcoR1, Pst1, AgeI, NgomIV, BsmbI →Phoneutria? *Jacques Nicoli→ Yeast *Biosafety (term 23/06) *Donation Fiocruz
4º week	*New England Enzyme *Texts Wiki →Project →Team →Human practices →Facebook →Vakinha (Crowd funding)
5º week
July
1º week	* Experimental tests * shopping * Restriction enzymes * length of 110 nucleotides linker → → try a smaller *experimental →Biobrick assemble the plasmid →Sequencing →Express and perform the tests in vitro. →Write protocols for wiki *Modeling mathematics *Wiki: →Assemble layout and content. *Human Practices →Questionnaires →Sponsorships →Questions - students ufmg schools. *Bioethics
2º week	*Price for synthesizing biobrick →Request assistance pro-reitoria *Modeling →test of interaction assemblies TALE + + Linker Hmcherry1 and 2 *Making a letter for sponsorship
3º week	*Deadlines * Fundraising Ideas * Human Practices → Send e-mails with the questionnaires * Modeling of proteins *Extraction of faeces
4º week
5º week
August
1º week	* Arrival of restriction enzyme → Work *Texts for wiki *inscription IGEM
2º week	*Experiments
3º week	*Sponsors
4º week	*Experiments * Human Practices →Results of the questionnaires * Modeling of proteins
5º week
September
1º week	*Meeting with Dr.Clélio Campolina, National minister of Science and Technology *Wiki
2º week	*Human Practices: low adhesion *Fetch data from investment in research *Participation in Latin America over the years in the iGem *Plot: X number of contacts feedback *Lack of appreciation of Science *Popular search
3º week
4º week	*Experiments *Project Summary *Meeting with Dr. Evaldo Vilela, FAPEMIG director.
5º week
October
1º week	*Finish the Wiki Modeling *Experimental *Human practices. →Questionnaire in the 7 square
2º week	*Lecture to high school, Technical school and Graduation, at CEFET. * Survey: The Importance of Science * Concentrations DNA * Sponsors * Deadlines
3º week	* Experiments →Results * Human Practices →Results *Modeling of proteins →Results * Modeling mathematics →Results * Sponsors →Tshirt →Who helped?
4º week
5º week
November
	World Championship Jamboree.