Team:Oxford/biopolymer containment
From 2014.igem.org
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1.5% agarose 'beads' were synthesised by dropping cooling (~40<font style="vertical-align: super;font-size: smaller;">o</font>C) 1.5% agarose solution through a 250 mL measuring cylinder of 0<font style="vertical-align: super;font-size: smaller;">o</font>C water, via 10mL Gilson pipette:<br><br> | 1.5% agarose 'beads' were synthesised by dropping cooling (~40<font style="vertical-align: super;font-size: smaller;">o</font>C) 1.5% agarose solution through a 250 mL measuring cylinder of 0<font style="vertical-align: super;font-size: smaller;">o</font>C water, via 10mL Gilson pipette:<br><br> | ||
<img src="https://static.igem.org/mediawiki/2014/d/d8/Oxigembeadsynth1.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /><br><br> | <img src="https://static.igem.org/mediawiki/2014/d/d8/Oxigembeadsynth1.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /><br><br> | ||
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To coat the product with cellulose acetate, a modified biopolymer, the solidified agarose beads were passed through the following biphasic mixture, a thin organic layer consisting of cellulose acetate in ethyl acetate above an aqueous layer: | To coat the product with cellulose acetate, a modified biopolymer, the solidified agarose beads were passed through the following biphasic mixture, a thin organic layer consisting of cellulose acetate in ethyl acetate above an aqueous layer: | ||
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<img src="https://static.igem.org/mediawiki/2014/9/93/Oxigembeadsynth3.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /><br><br> | <img src="https://static.igem.org/mediawiki/2014/9/93/Oxigembeadsynth3.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /><br><br> | ||
<img src="https://static.igem.org/mediawiki/2014/d/d0/Oxigembeadynth4.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /><br><br> | <img src="https://static.igem.org/mediawiki/2014/d/d0/Oxigembeadynth4.jpg" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /><br><br> | ||
+ | As of the wiki freeze, we had yet to perform polymer coating of bacteria-containing agarose beads, although have made arrangements within the Oxford's Biochemistry department to further research this, to be written as a scientific paper.<br><br> | ||
By collecting the resulting 'capsules' and repeating this procedure, we built up polymer coat thicknesses up to 5mm, calculated by the difference in measured initial and final diameters (an average of 5 diameters, using 0.01 mm precision callipers).<br><br> | By collecting the resulting 'capsules' and repeating this procedure, we built up polymer coat thicknesses up to 5mm, calculated by the difference in measured initial and final diameters (an average of 5 diameters, using 0.01 mm precision callipers).<br><br> | ||
<img src="https://static.igem.org/mediawiki/2014/d/da/Oxford_polymer4.png" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /> | <img src="https://static.igem.org/mediawiki/2014/d/da/Oxford_polymer4.png" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:2%;" /> |
Revision as of 02:06, 18 October 2014