Team:Groningen/Template/MODULE/Notebook/bandage/week9

From 2014.igem.org

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1 September - 4 September
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September 1 - September 4
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After several weeks of trying to pour cells inside the gel it's time to switch to another kind of experiments.
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Nisin diffusion tests performed with the polyacrylamide gel
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This time we will start by doing nisin diffusion tests. We'd like to see if nisin diffuses through our acrylamid gel and still spreads out to other bacteria.
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A GM17 agar plate with <i>L. lactis</i> NZ9000 on it, this is a <i>L. lactis</i> strain that is sensitive for nisin, after which we made three holes in the agar
how will we do this: We will start by pouring gm17 agar plates with the nz9000 strain on it, this is a strain that doesn't have any nisin resistance. While pouring we had 3 empty circles inside this agar plate where we can put in 3 small samples of acrylamid gel.
how will we do this: We will start by pouring gm17 agar plates with the nz9000 strain on it, this is a strain that doesn't have any nisin resistance. While pouring we had 3 empty circles inside this agar plate where we can put in 3 small samples of acrylamid gel.
This acrylamid gel contains the NZ9700 strain, this is a strain that can create nisin. It does this after induction with nisin.
This acrylamid gel contains the NZ9700 strain, this is a strain that can create nisin. It does this after induction with nisin.
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Inside the three holes, polyacrylamide gels with the <i>L. lactis</i> nisin producing strain NZ9700
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The acrylamid gels which we will put inside this agar plate have several variations. We can choose to induce them, but since it's a leaky promotor we will try it without inducing as well. One of the other varaiables is that we let is grow overnight in either water or media (GM17) and this will likely give a different result.
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The three holes had slightly different conditions, one of the three wholes if filled with a gel, which was incubated overnight at 30 °C, the other two were filled with a freshly prepared gel of which one is not induced to produce nisin
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Our first try didn't go to well because the gel didn't seem to touch the agar, diffusion wouldn't start.
Our first try didn't go to well because the gel didn't seem to touch the agar, diffusion wouldn't start.
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During the first expiremt we hardly had any results, because the gel did not cam in touch with the agar
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Revision as of 02:01, 18 October 2014

September 1 - September 4
 
Nisin diffusion tests performed with the polyacrylamide gel
 
A GM17 agar plate with L. lactis NZ9000 on it, this is a L. lactis strain that is sensitive for nisin, after which we made three holes in the agar how will we do this: We will start by pouring gm17 agar plates with the nz9000 strain on it, this is a strain that doesn't have any nisin resistance. While pouring we had 3 empty circles inside this agar plate where we can put in 3 small samples of acrylamid gel. This acrylamid gel contains the NZ9700 strain, this is a strain that can create nisin. It does this after induction with nisin.
 
Inside the three holes, polyacrylamide gels with the L. lactis nisin producing strain NZ9700
 
The three holes had slightly different conditions, one of the three wholes if filled with a gel, which was incubated overnight at 30 °C, the other two were filled with a freshly prepared gel of which one is not induced to produce nisin Our first try didn't go to well because the gel didn't seem to touch the agar, diffusion wouldn't start.
 
During the first expiremt we hardly had any results, because the gel did not cam in touch with the agar
 
 
 
 

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