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Team:Paris Bettencourt/Results
From 2014.igem.org
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<div class=project id=project2> | <div class=project id=project2> | ||
| - | <p class=text2 | + | <p class=text2></p> |
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell">Teen Spirit</a></h6><br> | <h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell">Teen Spirit</a></h6><br> | ||
| - | <p class=text1> | + | <p class=text1>‘<ul> |
| + | <li>Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.</li> | ||
| + | <li>Determined the critical smell detection limit of 2-nonenal.</li> | ||
| + | <li>Analyzed 2-nonenal tolerance and degradation in natural isolates.</li> | ||
| + | |||
| + | </ul></p> | ||
</div> | </div> | ||
<div class=project id=project3> | <div class=project id=project3> | ||
Revision as of 01:59, 18 October 2014
| Smell the roses |
Teen spirit |
Something fishy |
Goody two shoes |
Don't sweat it |
Smell The Roses
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Teen Spirit
‘
- Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.
- Determined the critical smell detection limit of 2-nonenal.
- Analyzed 2-nonenal tolerance and degradation in natural isolates.
Something Fishy
- Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
- Characterized and quantified the activity of TMM by a colorimetric assay.
- Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).
Goody Two Shoes
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Don't Sweat It
- Designed a BioBrick of agaA (main gene responsible for body odor) in pSB1C3.
- Found hotspots for genes ackA and ldh (responsible for body odor) found in human microbiome samples.
- Design CRISPRs to select for natural odorless strains.
- Made a DIY formulation of probiotic cream.
Human practice
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Interlab study
The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.iGEM Paris Bettencourt team
- - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
- - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
- - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
- - Sequenced and gel verified the three devices
- - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
- - Reported the results on the Interlab Study page of the iGEM wiki
- - Completed the description of the BBa_I20260 - Device 1
- - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry
Collaborations
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