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Team:Bordeaux/NotebookAugust20
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(Created page with "{{:Team:Bordeaux/Tete}} <html> <h1>20<sup>th</sup> August </h1> <div class="type-exp"> Molecular biology</div> <br> <ul class="day-detail"> <li> Test of pSB1C3 miniprep <ul cl...") |
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<li>Migration on 1% agarose gel (100V, 30min)</li></ul> | <li>Migration on 1% agarose gel (100V, 30min)</li></ul> | ||
| - | </li> | + | </li><br> |
<li> | <li> | ||
Making daily competent cells (BL21) and transformation with pSB1C3 (spread on LB+chlo plates) | Making daily competent cells (BL21) and transformation with pSB1C3 (spread on LB+chlo plates) | ||
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<ul class="day-detail"> | <ul class="day-detail"> | ||
| - | <li> | + | <li>End of the purification for ELP-40 and -60 |
| - | <div style="margin-left:40px;"> | + | <div style="margin-left:40px;">=> Begin of dialysis</div> |
</li> | </li> | ||
Latest revision as of 01:58, 18 October 2014
20th August
Molecular biology
-
Test of pSB1C3 miniprep
- Migration on 1% agarose gel (100V, 30min)
- Making daily competent cells (BL21) and transformation with pSB1C3 (spread on LB+chlo plates)
Production
Problem with preculture => ampicillin
Purification
- End of the purification for ELP-40 and -60
=> Begin of dialysis
