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Team:Paris Bettencourt/Results
From 2014.igem.org
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<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/TMAU">Something Fishy</a></h6><br> | <h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/TMAU">Something Fishy</a></h6><br> | ||
| - | <p class=text1> | + | <p class=text1>We created a new Biobrick by cloning <i>tmm</i> gene into a Biobrick vector and expressed it in <i>E. coli</i>. The activity of TMM was demonstrated both with a color test and a gas chromatography-mass spectrometer analysis, showing that trimethylamine is degraded and fish odor reduced. </p> |
</div> | </div> | ||
<div class=project id=project4> | <div class=project id=project4> | ||
Revision as of 01:53, 18 October 2014
| Smell the roses |
Teen spirit |
Something fishy |
Goody two shoes |
Don't sweat it |
Smell The Roses
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Figure 1.Main idea of 'Teen spirit'
Teen Spirit
‘Old people smell’ is a characteristic smell of people over 40 caused by the appearance of 2-nonenal in sweat. In this project we isolated and characterized natural bacteria that can metabolize 2-nonenal and therefore attenuate its smell. These bacteria could be used in a cosmetic product to reduce 2-nonenal in body odor.
Something Fishy
We created a new Biobrick by cloning tmm gene into a Biobrick vector and expressed it in E. coli. The activity of TMM was demonstrated both with a color test and a gas chromatography-mass spectrometer analysis, showing that trimethylamine is degraded and fish odor reduced.
Goody Two Shoes
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Don't Sweat It
- Designed a BioBrick of agaA (main gene responsible for body odor) in pSB1C3.
- Found hotspots for genes ackA and ldh (responsible for body odor) found in human microbiome samples.
- Design CRISPRs to select for natural odorless strains.
- Made a DIY formulation of probiotic cream.
Human practice
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Interlab study
The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.iGEM Paris Bettencourt team
- - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
- - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
- - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
- - Sequenced and gel verified the three devices
- - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
- - Reported the results on the Interlab Study page of the iGEM wiki
- - Completed the description of the BBa_I20260 - Device 1
- - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry
Collaborations
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+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com 
