Team:Clemson/Safety

From 2014.igem.org

(Difference between revisions)
Line 5: Line 5:
-
<html>
+
<center>'''Basic Safety Questions for iGEM 2013'''</center>
-
<!--main content -->
+
'''1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:  '''
-
<table width="70%" align="center">
+
 +
{| class="wikitable"
 +
|-
 +
!
 +
Species
-
<!--welcome box -->
+
!
-
<tr>
+
Strain no/name
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
-
<br>
+
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Clemson/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
-
</td>
+
-
</tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
!
-
<!-- end welcome box -->
+
Risk Group
-
<tr>
+
-
<!--navigation menu -->
+
!
-
<td align="center" colspan="3">
+
Risk group source link
-
<table  width="100%">
+
!
-
<tr heigth="15px"></tr>
+
Disease risk to humans? If so, which disease?
-
<tr heigth="75px">
+
 +
|-
 +
|
 +
''E. coli''
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
|
-
<a href="https://2014.igem.org/Team:Clemson"style="color:#000000">Home </a> </td>
+
DH10B
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
|
-
<a href="https://2014.igem.org/Team:Clemson/Team"style="color:#000000"> Team </a> </td>
+
1
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
|
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Clemson"style="color:#000000"> Official Team Profile </a></td>
+
[http://tools.invitrogen.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf http://tools.invitrogen.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf]
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
|
-
<a href="https://2014.igem.org/Team:Clemson/Project"style="color:#000000"> Project</a></td>
+
Yes. May cause eye and skin irritation with susceptible persons. May be harmful by inhalation. May be harmful if swallowed.
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
|-
-
<a href="https://2014.igem.org/Team:Clemson/Parts"style="color:#000000"> Parts</a></td>
+
|
 +
''E. coli''
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
|
-
<a href="https://2014.igem.org/Team:Clemson/Modeling"style="color:#000000"> Modeling</a></td>
+
K12
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
|
-
<a href="https://2014.igem.org/Team:Clemson/Notebook"style="color:#000000"> Notebook</a></td>
+
1
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
|
-
<a href="https://2014.igem.org/Team:Clemson/Safety"style=" color:#000000"> Safety </a></td>
+
[http://atcc.org/Products/All/29425.aspx#839CDF1D115D43D5AE9FEDD4CB8A9BE5 http://atcc.org/Products/All/29425.aspx#839CDF1D115D43D5AE9FEDD4CB8A9BE5]
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
|
-
<a href="https://2014.igem.org/Team:Clemson/Attributions"style="color:#000000"> Attributions </a></td>
+
Yes. May cause irritation to skin, eyes, and respiratory tract. May affect kidneys.
 +
|-
 +
|
 +
''E. coli''
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
|
-
</tr>
+
E. colni™
-
</table>
+
-
<!--end navigation menu -->
+
-
</tr>
+
-
</tr>
+
-
</td>
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
|
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
1
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
 +
|
 +
[http://lucigen.com/store/docs/literature/flyer/E-cloni-Cells-2011.pdf http://lucigen.com/store/docs/literature/flyer/E-cloni-Cells-2011.pdf]
-
<!--safety content-->
+
|
-
<tr><td > <h3> Welcome! </h3></td>
+
No
-
<td ></td >
+
-
<td > <h3> Timeline</h3></td>
+
-
</tr>
+
-
<tr>
+
|}
-
<td width="45%"  valign="top">
+
'''2. Highest Risk Group Listed:''' 1
-
<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
+
 +
'''3. List and describe ''all ''new or modified coding regions you will be using in your project.'''
 +
{| class="wikitable"
 +
|-
 +
!
 +
Part number.
-
<br>
+
-
<h3> Edit this page!</h3>
+
-
<p>
+
-
Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
+
-
<ul>
+
-
<li> <a href=" ">Learn about lab Safety for Today</a></li>
+
-
<li> <a href="">Learn about Safety for the future of your project.</a></li>
+
-
</ul>
+
-
-->
+
-
</p>
+
-
<h3> Your Lab </h3>
+
!
 +
Where did you get the physical DNA for this part (which lab,
 +
synthesis company, etc)
-
<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
+
!
-
<!--
+
What species does this part originally come from?
-
<gallery>
+
-
Image:Example2_Lab_1.png|The building our lab is in!
+
-
Image:Example2_Lab_2.png|The inside of our lab!
+
-
Image:Example2_Lab_3.png|Team Member 3 doing an experiment
+
-
Image:Example2_Lab_4.png|Working in biosafety cabinets
+
-
Image:Example2_Lab_5.png|Team all gloved up and ready for work!
+
-
Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
+
-
Image:Example2_Lab_7.png|We decorated this part of our lab
+
-
Image:Example2_Lab_8.png|Whatever else you want
+
-
</gallery>-->
+
-
</td>
+
!
-
<td></td>
+
What is the Risk Group of the species?
 +
!
 +
What is the function of this part, in its parent species? 
-
<td width="45%"  valign="top">
+
|-
 +
|
 +
NA
 +
|
 +
PCR amplification and site directed mutagenesis
-
<ul>
+
|
-
<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
+
''E. coli ''K12
-
<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
+
-
<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
+
-
<li><b>June 9: </b>Submit the About Our Lab form.</li>
+
-
<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
+
-
<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
+
-
<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
+
-
<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
+
-
<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
+
-
<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
+
-
</ul>
+
|
-
</td>
+
1
-
</tr>
+
|
 +
Beta-galactosidase
 +
|-
 +
|
 +
K592009
 +
|
 +
iGEM
 +
|
 +
''Acropora millepora''
-
<tr>
+
|
 +
NA
 +
|
 +
Blue pigment
 +
|}
 +
'''4. Do the biological materials used in your lab work pose any of the following risks? Please describe. '''
 +
'''a. Risks to the safety and health of team members or others working in the lab? '''
 +
{| class="prettytable"
 +
|-
 +
|
 +
All works are carried out following standard BSL-1 practice in BSL-1 facilities.
 +
|}
 +
'''b. Risks to the safety and health of the general public, if released by design or by accident? '''
 +
{| class="prettytable"
 +
|-
 +
|
 +
Access to laboratory facilities and project materials are restricted to team members only.  There is no planned release of engineered microorganisms.  All bacterial strains used in this current project are not considered pathogenic and do not cause diseases in healthy adults.
 +
|}
 +
'''c. Risks to the environment, if released by design or by accident? '''
-
</table>
+
{| class="prettytable"
-
</html>
+
|-
 +
|
 +
There is no planned release of engineered microorganisms to the environment in this current project.  All bacterial strains used are either laboratory strains that do not survive outside of laboratory environment or are wild type bacterial that normally present in the environment.
 +
 
 +
|}
 +
'''d. Risks to security through malicious misuse by individuals, groups, or countries? '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
All biological agents utilized in this current project are not on the Selection Agent list and do not pose any biosecurity threats.
 +
 
 +
|}
 +
'''5. If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
The current biological constructs do not pose risks and there is no scale-up plan in this current project.  However, the risks associated with large-scale production of the biological constructs will be assessed when such plan for commercialization arises.
 +
 
 +
|}
 +
'''6. Does your project include any design features to address safety risks? (For example: kill switches, auxotrophic chassis, etc.) Note that including such features is not mandatory to participate in iGEM, but many groups choose to include them. '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
The current project is in the proof-of-concept stage.  Kill switches and/or auxotrophic chassis will be included in future design.
 +
 
 +
|}
 +
'''7. What safety training have you received (or plan to receive in the future)?  Provide a brief description, and a link to your institution’s safety training requirements, if available. '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
All iGEM members are required to complete training modules located at [http://www.clemson.edu/research/safety/training/ http://www.clemson.edu/research/safety/training/] and [https://www.citiprogram.org/ https://www.citiprogram.org] before working in the laboratory.
 +
 
 +
|}
 +
'''8. Under what biosafety provisions will / do you work? '''
 +
 
 +
'''a. Please provide a link to your institution biosafety guidelines. '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
All works are carried out following Clemson University's chemical hygiene plan and biosafety guidelines overseen by Environmental Health and Safety, [http://ehs.clemson.edu/ http://ehs.clemson.edu/], and Institutional Biosafety Committee, [http://www.clemson.edu/research/compliance/ibc/ http://www.clemson.edu/research/compliance/ibc/].
 +
 
 +
|}
 +
'''b. Does your institution have an Institutional Biosafety Committee, or an equivalent group? If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project plan based on their review. '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
The primary instructor of Clemson iGEM team is a member of the IBC and is in constant communication with members of the committee.  No concerns have been raised regarding this current project.
 +
 
 +
|}
 +
'''c. Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible. '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
The relevant national biosafety regulations and policies are listed on [http://www.clemson.edu/research/compliance/ibc/regulations.html http://www.clemson.edu/research/compliance/ibc/regulations.html].
 +
 
 +
|}
 +
'''d. According to the WHO Biosafety Manual, what is the BioSafety<nowiki> Level rating of your lab? (Check the summary table on page 3, and the fuller description that starts on page 9.) If your lab does not fit neatly into category 1, 2, 3, or 4, please describe its safety features [see 2013.igem.org/Safety for help]. </nowiki>'''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
All research activities related to current project are carried out in BSL-1 rated laboratory spaces even though some of the iGEM members have clearance for access to BSL-2 rated laboratory spaces located in the same Life Sciences Facility.
 +
 
 +
|}
 +
'''e. What is the Risk Group of your chassis organism(s), as you stated in question 1? If it does not match the BSL rating of your laboratory, please explain what additional safety measures you are taking. '''
 +
 
 +
{| class="prettytable"
 +
|-
 +
|
 +
Only RG-1 microorganisms are utilized in the current project.
 +
 
 +
|}

Revision as of 01:52, 18 October 2014



Basic Safety Questions for iGEM 2013

1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:

Species

Strain no/name

Risk Group

Risk group source link

Disease risk to humans? If so, which disease?

E. coli

DH10B

1

[http://tools.invitrogen.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf http://tools.invitrogen.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf]

Yes. May cause eye and skin irritation with susceptible persons. May be harmful by inhalation. May be harmful if swallowed.

E. coli

K12

1

[http://atcc.org/Products/All/29425.aspx#839CDF1D115D43D5AE9FEDD4CB8A9BE5 http://atcc.org/Products/All/29425.aspx#839CDF1D115D43D5AE9FEDD4CB8A9BE5]

Yes. May cause irritation to skin, eyes, and respiratory tract. May affect kidneys.

E. coli

E. colni™

1

[http://lucigen.com/store/docs/literature/flyer/E-cloni-Cells-2011.pdf http://lucigen.com/store/docs/literature/flyer/E-cloni-Cells-2011.pdf]

No

2. Highest Risk Group Listed: 1

3. List and describe all new or modified coding regions you will be using in your project.

Part number.


Where did you get the physical DNA for this part (which lab,

synthesis company, etc)

What species does this part originally come from?

What is the Risk Group of the species?

What is the function of this part, in its parent species?

NA

PCR amplification and site directed mutagenesis

E. coli K12

1

Beta-galactosidase

K592009

iGEM

Acropora millepora

NA

Blue pigment

4. Do the biological materials used in your lab work pose any of the following risks? Please describe.

a. Risks to the safety and health of team members or others working in the lab?

All works are carried out following standard BSL-1 practice in BSL-1 facilities.

b. Risks to the safety and health of the general public, if released by design or by accident?

Access to laboratory facilities and project materials are restricted to team members only. There is no planned release of engineered microorganisms. All bacterial strains used in this current project are not considered pathogenic and do not cause diseases in healthy adults.

c. Risks to the environment, if released by design or by accident?

There is no planned release of engineered microorganisms to the environment in this current project. All bacterial strains used are either laboratory strains that do not survive outside of laboratory environment or are wild type bacterial that normally present in the environment.

d. Risks to security through malicious misuse by individuals, groups, or countries?

All biological agents utilized in this current project are not on the Selection Agent list and do not pose any biosecurity threats.

5. If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise?

The current biological constructs do not pose risks and there is no scale-up plan in this current project. However, the risks associated with large-scale production of the biological constructs will be assessed when such plan for commercialization arises.

6. Does your project include any design features to address safety risks? (For example: kill switches, auxotrophic chassis, etc.) Note that including such features is not mandatory to participate in iGEM, but many groups choose to include them.

The current project is in the proof-of-concept stage. Kill switches and/or auxotrophic chassis will be included in future design.

7. What safety training have you received (or plan to receive in the future)? Provide a brief description, and a link to your institution’s safety training requirements, if available.

All iGEM members are required to complete training modules located at [http://www.clemson.edu/research/safety/training/ http://www.clemson.edu/research/safety/training/] and https://www.citiprogram.org before working in the laboratory.

8. Under what biosafety provisions will / do you work?

a. Please provide a link to your institution biosafety guidelines.

All works are carried out following Clemson University's chemical hygiene plan and biosafety guidelines overseen by Environmental Health and Safety, [http://ehs.clemson.edu/ http://ehs.clemson.edu/], and Institutional Biosafety Committee, [http://www.clemson.edu/research/compliance/ibc/ http://www.clemson.edu/research/compliance/ibc/].

b. Does your institution have an Institutional Biosafety Committee, or an equivalent group? If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project plan based on their review.

The primary instructor of Clemson iGEM team is a member of the IBC and is in constant communication with members of the committee. No concerns have been raised regarding this current project.

c. Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible.

The relevant national biosafety regulations and policies are listed on [http://www.clemson.edu/research/compliance/ibc/regulations.html http://www.clemson.edu/research/compliance/ibc/regulations.html].

d. According to the WHO Biosafety Manual, what is the BioSafety Level rating of your lab? (Check the summary table on page 3, and the fuller description that starts on page 9.) If your lab does not fit neatly into category 1, 2, 3, or 4, please describe its safety features [see 2013.igem.org/Safety for help].

All research activities related to current project are carried out in BSL-1 rated laboratory spaces even though some of the iGEM members have clearance for access to BSL-2 rated laboratory spaces located in the same Life Sciences Facility.

e. What is the Risk Group of your chassis organism(s), as you stated in question 1? If it does not match the BSL rating of your laboratory, please explain what additional safety measures you are taking.

Only RG-1 microorganisms are utilized in the current project.

Retrieved from "http://2014.igem.org/Team:Clemson/Safety"