Team:British Columbia/ProjectBiomining

From 2014.igem.org

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We, the UBC iGEM team, feel that separation and enrichment can be done in other ways that does not rely on energy or chemically-intensive methods. Our synbio solution involves the use of small surface heptapeptides and octapeptides, which have previously been demonstrated in M13 bacteriophage by Curtis et al. to selectively bind to chalcopyrite(3). Three peptides, labelled WSD-1 (TPTTYKV), WSD-2 (DSQKTNPS), and WSD-3 (DPIKHTSG), have been identified for binding. However, operating with bacteriophage is not feasible for large scale operations in mining as it is difficult to scale up titers to compensate for the smaller surface area available for binding in bacteriophage. Our idea is to operate these peptides in bacteria which have a larger surface area and are much more responsive to stimuli. These peptides must be accessible on the surface. Therefore, we have chosen ''Caulobacter crescentus'' as the chosen chassis given that it contains a S-protein layer in which we can express our peptides. A developed kit for protein secretion and display of peptides on the cell surface S-layer in ''Caulobacter crescentus''  (via cloning to the S-layer gene sequence) can be found at  
We, the UBC iGEM team, feel that separation and enrichment can be done in other ways that does not rely on energy or chemically-intensive methods. Our synbio solution involves the use of small surface heptapeptides and octapeptides, which have previously been demonstrated in M13 bacteriophage by Curtis et al. to selectively bind to chalcopyrite(3). Three peptides, labelled WSD-1 (TPTTYKV), WSD-2 (DSQKTNPS), and WSD-3 (DPIKHTSG), have been identified for binding. However, operating with bacteriophage is not feasible for large scale operations in mining as it is difficult to scale up titers to compensate for the smaller surface area available for binding in bacteriophage. Our idea is to operate these peptides in bacteria which have a larger surface area and are much more responsive to stimuli. These peptides must be accessible on the surface. Therefore, we have chosen ''Caulobacter crescentus'' as the chosen chassis given that it contains a S-protein layer in which we can express our peptides. A developed kit for protein secretion and display of peptides on the cell surface S-layer in ''Caulobacter crescentus''  (via cloning to the S-layer gene sequence) can be found at  
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<a href="http://ubc.flintbox.com/public/project/1487/">Caulobacter S-layer Kits</a>
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<a href="http://ubc.flintbox.com/public/project/1487/">Caulobacter S-layer Kits</a>.
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   </p>

Revision as of 01:44, 18 October 2014

2014 UBC iGEM

© 2014 UBC iGEM