Team:UC-Santa Cruz-BioE/Notebook

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<li>1. Inoculated TSB with MR-1 (either from plate or glycerol stock) and grow overnight.</li>
<li>1. Inoculated TSB with MR-1 (either from plate or glycerol stock) and grow overnight.</li>
<li>2. add glycerol to culture to final concentration of 80% glycerol.</li>
<li>2. add glycerol to culture to final concentration of 80% glycerol.</li>
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<li>3. place 1ml in 1.5ml freezer safe eppendorf tubes (do not fill eppendorfs with more than 1ml to allow for expansion by freezing).</li>
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<li>3. place 1ml in 1.5ml freezer safe eppendorf tubes (do not fill eppendorfs with more than 1.5ml to allow for expansion by freezing).</li>
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<li>1.  cut two 3 inch clear PVC tubes .</li>
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<li>1.  cut two 3 inch PVC tubes .</li>
<li>2.  sandwich them with nafion cation exchange membrane between them.</li>
<li>2.  sandwich them with nafion cation exchange membrane between them.</li>
<li>3.  add caulking around both sides of membrane-tube junction.</li>
<li>3.  add caulking around both sides of membrane-tube junction.</li>
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<h3>Plasmid Prep</h3>
<h3>Plasmid Prep</h3>
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Revision as of 01:42, 18 October 2014


Notebook

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Important Protocols

Glycerol Stock Preparation

  • 1. Inoculated TSB with MR-1 (either from plate or glycerol stock) and grow overnight.
  • 2. add glycerol to culture to final concentration of 80% glycerol.
  • 3. place 1ml in 1.5ml freezer safe eppendorf tubes (do not fill eppendorfs with more than 1.5ml to allow for expansion by freezing).

Microbial Fuel Cell Assembly

  • 1. cut two 3 inch PVC tubes .
  • 2. sandwich them with nafion cation exchange membrane between them.
  • 3. add caulking around both sides of membrane-tube junction.
  • 4. let dry for 15 minutes.
  • 5. repeat step 3 and 4.
  • 6. cut off excess membrane around junction.
  • 7. add one more layer to seal up junction.
  • 8. let dry overnight.
  • 9. cut 2.5in-3in x 12in of carbon cloth
  • 10. roll around tip of finger.
  • 11. weave titanium wire close to end edge of roll.
  • 12 fold titanium wire up the length of the roll.
  • 13. twist excess wire around itself.
  • 14. cut wire at .5in-1in.
  • 15. push through stopper.
  • 16. seal hole from wire with caulking.
  • 17. let dry overnight. (two of these make up the cathode and the anode)
  • 18. pour potassium ferricyanide in one chamber of the cell.
  • 19. carefully push stopper with carbon cloth to plug the chamber.
  • 20. push hollow needle through stopper to let air out of chamber.
  • 21. pour overnight culture of MR-1 into other chamber.
  • 22. place glass slide in chamber.
  • 23. carefully push stopper with carbon cloth to plug the chamber.
  • 24. push new sterile hollow needle in stopper into air bubble in chamber to let out any CO2 bacteria will produce.
  • 25. open ExcelINX up on computer.
  • 26. connect electrodes from DMM to cathode and anode (remember which channels are being used).
  • 27. start ExcelINX.

Plasmid Prep

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