Team:Korea U Seoul/Notebook sub

From 2014.igem.org

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<li>Minseob - cell to cell communication</li>
<li>Minseob - cell to cell communication</li>
<p>- Outer membrane vesicles (OMV) : gram negative bacteria like E. coli have Outer membrane vesicles (OMV), which can transfer toxin to other bacteria or host strains. Omv is composed of bi-lipid layer with water soluble core, so it can transfer both water soluble materials and lipid soluble materials. Omv is a strong candidate in long-distance messaging.</p>
<p>- Outer membrane vesicles (OMV) : gram negative bacteria like E. coli have Outer membrane vesicles (OMV), which can transfer toxin to other bacteria or host strains. Omv is composed of bi-lipid layer with water soluble core, so it can transfer both water soluble materials and lipid soluble materials. Omv is a strong candidate in long-distance messaging.</p>
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<li>Pick a single, well-isolated colony and inoculate it into 5 ml of LB broth (plus antibiotic). Incubate at 37 oC overnight with shaking at 220 rpm.</li>
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<li>Hyeyeong -sensitivity tuners, color generator</li>
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<li>Transfer 1 ml of the saturated overnight culture to a sterile 500-ml flask containing 100 ml of LB medium (do not add antibiotic at this step). Incubate the cells at 37 oC with the shaking at 220 rpm, until OD600 reach 0.5 ; this usually takes 2.0-2.5 hr. Check the OD frequently when it gets beyond 0.2 to avoid overgrowth.</li>
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<p>- Different color expression by density</p>
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<li>When the culture reaches an OD600 of 0.5, chill the flask on the ice for 20 min and then collect the cells by centrifugation at 1500 rpm for 5 min at 4 oC.</li>
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<li>Juyoung –biosensor by density</li>
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<li>Resuspend the cells in 10 ml of ice-cold TSS solution. Now the competent cells are ready to be transformed.</li>
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<p>- Check the expression by density divided strong, wild, weak Lock & key, Jammer</p>
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<li>Aliquot 150 ul competent cells to 1.5ml tube. If they are not immediately used, cells can be stored at 4 oC for maximum of 6 hr without significant loss of competency. The same competent cells can also be stored at -70 oC for long-term storage (pre-treat with liquid nitrogen or dry ice).</li>
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<li>Heonsong – estimating stress by promoter & microorganism which can produce materials when get some light.</li>
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<p>
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<p>- Formation of hybrid-promoter, light-sensing, biomaterial production</p>
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- (p.s.) Competent cells should give a minimum of 1x106 transformants per ug of plasmid DNA. Transformation frequency of frozen cells is 30 % of that of the fresh cells, when used within two months.
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<li>Jihee- Biothermometer using RNA, not protein</li>
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</p>
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<p>- RNA double helix come untied when increase the heat.</p>
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<li>Kyugin- treat for kidney disease</li>
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<p>- using NhaA, Urea sensor</p>
</ol><br />
</ol><br />
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<p>- First meeting with 2014 kuas team members. Discussed about what is kuas, synthetic biology and igem.</p><br />
 
<p style="font-weight: bold; font-size:20px;">
<p style="font-weight: bold; font-size:20px;">
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Information
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Tips of instrutor
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</p><br />
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<p>- Synthetic biology homepage : <a href="http://syntheticbiology.org">http://syntheticbiology.org</a></p><p>- Part legistry page : <a href="http://part.igem.org">http://part.igem.org</a>
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</p><br />
</p><br />
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<ol type="1"/>
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<li>Categorize the theme. </li>
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<li>Regulate the level. </li>
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<li>Refer other colleges access methods. </li>
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</ol><br />
<p style="font-weight: bold; font-size:20px;">
<p style="font-weight: bold; font-size:20px;">
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Homework
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Discussion of direction
</p><br />
</p><br />
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<p>- investigate about subject of2008, 2009 igem teams investigate about subject of 2008, 2009 igem teams</p><br />
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<ol type="1"/>
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             </div>    
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<li>Take category- one of food/energy, environment, manufacturing. </li>
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<li>Investigate some ideas food & energy part until 2013.12.23</li>
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</ol><br />
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             <div class="title">
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                   April
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                   2014 IGEM 4th minutes
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                <p><span>1. Ideas</span><br/><span>(1) Keywords&nbsp;</span><br/><span>- We made bulletin board at our website that we can easily search ideas</span><br/><span></span><br/><span>Kyengwoo Jang : Cyanobacteria+Rhodopsin, Acetic acid</span><br/><span>Junhong Jang : MSG</span><br/><span>Hyunsong Kim : Shwanella+Nano Silver</span><br/><span>Juyoung Han : Aluminum</span><br/><span>Jihoon Jeong : Proteorhodopsin</span><br/><span>Jihee Park : Endospore inner membrane</span><br/><span>Hyeyeong Choi : Food waste odor, Nuclear waste breakdown</span><br/><span></span><br/><span>(2) Teamwork</span><br/><span>-We made team to think systemically</span><br/><span></span><br/><span>Group 1 (Jihoon Jeong, Minseob Yoo) : CaCO3, AR system</span><br/><span>Group 2 (Jihee Park, Hyeyeong Choi, Kyengwoo Jang, Minseob Yoo, Juyoung Han) : Nonylphenol, SPR sensor, Lignin &beta;-keto adipate pathway</span><br/><span>Group 3 (Junhong Jang, Kyengwoo Jang, Hyunsong Kim, Juyoung Han) : Bio-hoil</span><br/><span>Group 4 (Jihoon Jeong, Jaeuk Han, Minseob Yoo, Dongha Kim) : Bio-wire</span><br/><span></span><br/><span>We consolidated group 3&amp;4 to the topic of nano metal producing by microbes</span><br/><span></span><br/><span></span><br/><span>2. Human Practice</span><br/><span>- Vote for Best 3 Human Practice from last year : Cornell, Calgary, LMU-Munich</span></p>
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Study presentation
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</p><br />
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<ol type="1"/>
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            <div class="title">
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<li>JUYOUNG</li>
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                <div class="title_">
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<p>- Siderosphore : Fe3+ absorb, high reducing agent. =>use ocean microbes to greenhouse effect, pathogen control, biofuel</p>
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                  May
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<p>- Degradation Aplatoxin</p>
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<li>MINSEOB</li>
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                <div class="left_line"></div><div class="right_line"></div>
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<p>- GABA  :  neuro-material.</p>
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<p>- T3SS(Type 3 secretion system) : related to plant immunity. Mechanism has been found. </p>
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            <div class="content_">
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<p>- Pathogen microorganism sensor : use metabolic product as marker like enterotoxin or virulence factor</p>
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            <p>
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<p>- PCN(antifungal product) </p>
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                <p><span>1. Decide topic</span><br/><span>- Minseob Yoo</span><br/><span>: conchiolin is major protein of pearl</span><br/><span>There are two types of crystal structures of CaCO3, calcite and aragonite</span><br/><span>Nacerin automatically gathers and produce aragonite</span><br/><span></span><br/><span>We will further search :</span><br/><span>1) How CaCO3 makes aragonite</span><br/><span>2) Conditions for gloss</span><br/><span>3) Exact mechanism of making pearl by pearl shell</span><br/><span>4) Method of making layers</span><br/><span>5) Accurate role of colchiolin and possibility of producing it.</span><br/><span></span><br/><span></span><br/><span>2. Human Practice</span><br/><span>- Undergraduate essay contest : K water, Bioneer challenge, Samsung human-tech</span></p>
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<p>- Shiga toxin producing E. coli : can develop to cell to cell communication. Herein. </p>
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            </p>
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<p>- checker of cosmetics: ex) PH sensor</p>
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            </div>
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<li>JIHEE</li>
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            <div class="title">
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<p>- dye : E. coli producing indigo</p>
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                <div class="title_">
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<p>- Pectinase+ beta-chrotin, E. coli producing 3-4 nutrients-> making juice</p>
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                  June
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<li>HYEYEONG</li>
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<p>- bio-filter</p>
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                <div class="left_line"></div><div class="right_line"></div>
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<p>- removal odors of food junks : ex)remove pathogenity. </p>
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<p>- Soil sensor</p>
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            <div class="content_">
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</ol><br />
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<p style="font-weight: bold; font-size:20px;">
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                <p><span>Things to do</span><br/><span>- Decide team logo &amp; Order team T-shirt</span><br/><span>- Homepage making (Contents)</span><br/><span>- Festival at Yongsan youth center : Dongha Kim, Hyunsong Kim, Jihee Park</span></p>
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Next week
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</p><br />
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<ul>
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            <div class="title">
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<li>minseob- GABA, Shiga toxin, components of cosmetics</li>
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                <div class="title_">
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<li>jihee- effectiveness of making juice</li>
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                  July
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<li>hyeyeong- effectiveness of removing bad smells and probability of removing pathogenity</li>
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                </div>
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<li>juyeong- about iron ion, removing ground torpedo microbes</li>
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                <p><span>1. New name for Korea University biosynthetic academy</span><br/><span>- Korea University Association of Synthetic biology (KUAS)</span><br/><span></span><br/><span>2. Role allocation</span><br/><span>1) Sponsor :</span><br/><span>in the school - Jihoon Jeong</span><br/><span>outside the school - Dongha Kim, Junhong Jang, Jonghee Chun</span><br/><span>2) Writing reports : Juyoung Han</span><br/><span>3) CCP : Jihee Park, Hyunsong Kim, Juyoung Han, Minseob Yoo</span><br/><span>4) Plans in Hongkong : Jihoon Jeong</span><br/><span>5) Translation into English : Hyunsong Kim</span><br/><span>6) Wiki contents : Minseob Yoo, Kyengwoo Jang, Jaeuk Han</span></p>
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                  August
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                <p><span>1. Individual tasks</span><br/><span>1) Jihoon Jeong - Report, Plane ticket, Wiki contents</span><br/><span>2) Jaeuk Han - Wiki contents, Experiment</span><br/><span>3) Hyeyeong Choi - Human Practice in Africa</span><br/><span>4) Jihee Park - CCP, Experiment, Travel plan</span><br/><span>5) Juyoung Han - Report, Design, Manager</span><br/><span>6) Kyengwoo Jang - Wiki contents, Experiment</span><br/><span>7) Junhong Jang - Experiment, Sponsor</span><br/><span>8) Jonghee Chun - Sponsor, Homepage</span><br/><span>9) Minseob Yoo - Experiment, CCP, Wiki contents</span><br/><span>10) Dongha Kim - Homepage, Wiki platform</span><br/><span>11) Minwhan Yoo - Homepage, wiki platform</span><br/><span>12) Hyunsong Kim - Translation, Experiment, CCP</span><br/><span></span><br/><span>* Experiment day of week&nbsp;</span><br/><span>- Monday : Jaeuk Han</span><br/><span>- Tuesday : Jihee Park</span><br/><span>- Wednesday : Juyoung Han</span><br/><span>- Thursday : Hyunsong Kim</span></p>
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Revision as of 01:19, 18 October 2014

2014 IGEM 1st minutes

Meeting


- First meeting with 2014 kuas team members. Discussed about what is kuas, synthetic biology and igem.


Information


- Synthetic biology homepage : http://syntheticbiology.org

- Part legistry page : http://part.igem.org


Homework


- investigate about subject of2008, 2009 igem teams investigate about subject of 2008, 2009 igem teams


2014 IGEM 2nd minutes

Study of igem 2008~2009 colleges


  1. Minseob - cell to cell communication

    2. - Outer membrane vesicles (OMV) : gram negative bacteria like E. coli have Outer membrane vesicles (OMV), which can transfer toxin to other bacteria or host strains. Omv is composed of bi-lipid layer with water soluble core, so it can transfer both water soluble materials and lipid soluble materials. Omv is a strong candidate in long-distance messaging.

  2. Hyeyeong -sensitivity tuners, color generator

    3. - Different color expression by density

  3. Juyoung –biosensor by density

    4. - Check the expression by density divided strong, wild, weak Lock & key, Jammer

  4. Heonsong – estimating stress by promoter & microorganism which can produce materials when get some light.

    5. - Formation of hybrid-promoter, light-sensing, biomaterial production

  5. Jihee- Biothermometer using RNA, not protein

    6. - RNA double helix come untied when increase the heat.

  6. Kyugin- treat for kidney disease

    7. - using NhaA, Urea sensor


Tips of instrutor


  1. Categorize the theme.
  2. Regulate the level.
  3. Refer other colleges access methods.

Discussion of direction


  1. Take category- one of food/energy, environment, manufacturing.
  2. Investigate some ideas food & energy part until 2013.12.23

2014 IGEM 4th minutes

Study presentation


  1. JUYOUNG

    2. - Siderosphore : Fe3+ absorb, high reducing agent. =>use ocean microbes to greenhouse effect, pathogen control, biofuel

    - Degradation Aplatoxin

  2. MINSEOB

    3. - GABA : neuro-material.

    - T3SS(Type 3 secretion system) : related to plant immunity. Mechanism has been found.

    - Pathogen microorganism sensor : use metabolic product as marker like enterotoxin or virulence factor

    - PCN(antifungal product)

    - Shiga toxin producing E. coli : can develop to cell to cell communication. Herein.

    - checker of cosmetics: ex) PH sensor

  3. JIHEE

    4. - dye : E. coli producing indigo

    - Pectinase+ beta-chrotin, E. coli producing 3-4 nutrients-> making juice

  4. HYEYEONG

    5. - bio-filter

    - removal odors of food junks : ex)remove pathogenity.

    - Soil sensor


Next week


  • minseob- GABA, Shiga toxin, components of cosmetics
  • jihee- effectiveness of making juice
  • hyeyeong- effectiveness of removing bad smells and probability of removing pathogenity
  • juyeong- about iron ion, removing ground torpedo microbes

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