Team:UIUC Illinois/Protocols

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  <h1 style="margin-top:20px;margin-bottom:5px">Menu</h1>
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  <div class="leftside">
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    <ul>
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      <li><a onclick="showprot('#prot1')">Gel Eclectrophoresis</a></li>
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      <li><a onclick="showprot('#prot2')">PCR Set-Up</a></li>
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      <li><a onclick="showprot('#prot3')">Bacterial Transformation</a></li>
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      <li><a onclick="showprot('#prot4')">Restriction Endonuclease Digest</a></li>
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      <li><a onclick="showprot('#prot5')">CaCl2 Competent Cell Protocol</a></li>
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      <li><a onclick="showprot('#prot6')">Genomic DNA Purification</a></li>
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      <li><a onclick="showprot('#prot7')">Resting Cell Assay</a></li>
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      <li><a onclick="showprot('#prot8')">Colony PCR</a></li>
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      <li><a onclick="showprot('#prot9')">Lactobacillus Transformation</a></li>
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    <div id="prot1" class="protocolactive">
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      <a href="http://www.addgene.org/plasmid-protocols/gel-electrophoresis/"><h2> Gel Electrophoresis</h2></a>
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      <p>Summarize Protocol Here</p>   
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    </div>
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    <div id="prot2" class="protocol">
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      <a href="https://www.neb.com/protocols/1/01/01/pcr-protocol-m0530"><h2> PCR Set-Up</h2></a>
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      <p>Summary Paragraph</p>
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</div>
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<div id="prot3" class="protocol">
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      <a href="http://www.addgene.org/plasmid-protocols/bacterial-transformation/"><h2> Bacterial Transformation</h2></a>
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      <p>Summarize Protocol Here</p>   
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    </div>
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<div id="prot4" class="protocol">
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      <a href="https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions"><h2> Restriction Endonuclease Digest</h2></a>
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      <p>Summary Paragraph</p>
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</div>
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<div id="prot5" class="protocol">
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      <a href="http://www.med.nyu.edu/medicine/labs/blaserlab/Protocols/Ecoli_competent_cells_protocol_&_transformation.pdf"><h2> CaCl2 Competent Cell Protocol</h2></a>
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      <p>Summarize Protocol Here</p>   
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    </div>
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<div id="prot6" class="protocol">
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      <a href="http://www.promega.com/~/media/files/resources/protcards/wizard%20genomic%20dna%20purification%20kit%20quick%20protocol.pdf"><h2> Genomic DNA Purification</h2></a>
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      <p>Summary Paragraph</p>
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</div>
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<div id="prot7" class="protocol">
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      <a href="http://pubs.acs.org/doi/pdf/10.1021/sb4000146"><h2> Resting Cell Assay</h2></a>
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      <p>Summarize Protocol Here</p>   
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    </div>
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<div id="prot8" class="protocol">
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      <a href="http://www.csun.edu/~mls42367/Protocols/Colony%20PCR.pdf"><h2> Colony PCR</h2></a>
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      <p>Summary Paragraph</p>
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</div>
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<div id="prot9" class="protocol">
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      <h2> Lactobacillus Transformation</h2>
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      <p>
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<ol>
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<li>Inoculate 100mL MRS with 1mL of an overnight culture and incubate at 37C until it reaches an OD600 = 0.5 – 0.8.<br>
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<p style="text-indent:30px;">
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a. Pre-warm the MRS for faster growth
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b. Take 3.5-5.5 hours to get to 0.5
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c. All liquid cultures can be done outside the anaerobic chamber; however, do not use a shaker
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</p>
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</li>
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</ol>
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</p>
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Revision as of 01:15, 18 October 2014


Menu

Gel Electrophoresis

Summarize Protocol Here

PCR Set-Up

Summary Paragraph

Bacterial Transformation

Summarize Protocol Here

CaCl2 Competent Cell Protocol

Summarize Protocol Here

Genomic DNA Purification

Summary Paragraph

Resting Cell Assay

Summarize Protocol Here

Colony PCR

Summary Paragraph

Lactobacillus Transformation

  1. Inoculate 100mL MRS with 1mL of an overnight culture and incubate at 37C until it reaches an OD600 = 0.5 – 0.8.

    a. Pre-warm the MRS for faster growth b. Take 3.5-5.5 hours to get to 0.5 c. All liquid cultures can be done outside the anaerobic chamber; however, do not use a shaker


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