Team:UChicago/Safety

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UChicago/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<p>Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3>Our Lab</h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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<p>Our lab is a WHO BSL1 Lab, a low risk lab. We use a range of equipment from open areas, to laminar flow hoods, as well as standard lab safety practices. Cells cultures are killed with bleach before disposal, and general solid waste is collected into biohazard bags and disposed of.</p>
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<p>All team members recieved training on biosafety levels, requirements for laboratory practices and equipment, biohazard containment, recombinant DNA, bioethics, fire safety, and personal protective equipment. Our institution's safety training policies are available <a href="http://researchadmin.uchicago.edu/docs/ibc/UC_Biosafety_Manual.pdf">here</a>.
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<p>At our institution, we are supervised by the University of Chicago Office of Environmental Health and Safety. They provide a<a href="http://safety.uchicago.edu/pp/labsafety/index.shtml">form</a> as a guide to safety here. We are also under US law, which provides other guidelines <a href="http://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf">here</a>.</p>
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<p>All of the strains that we are using are in risk group 1, with the potential to cause irritation, or affect kidneys</p>
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<p>Specific safety concerns and responses include:
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<a href="https://2014.igem.org/Team:UChicago"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:UChicago/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UChicago"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:UChicago/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:UChicago/Attributions"style="color:#000000"> Attributions </a></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<li> <a href=" ">Learn about lab Safety for Today</a></li>
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<li>E. coli MG1655 and DH5a, which belong to risk group 1 and may pose the following risks: irritation to skin, eyes, and respiratory tract, may affect kidneys. All plates and cultures are disinfected with bleach after use, and the lab benches are wiped down in 70% EtOH and then dH2O to prevent contamination.</li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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<li>Some toxic or harmful chemicals such as ethidium bromide, chloroform and bleach. Team members are required to wear rubber gloves at all times in labs, and lab coats when handling harmful chemicals.</li>
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Other flammable chemicals such as isopropyl ethanol, ethanol, DMSO will be handled with care and jf spilled, the liquids will be soaked up and the lab bench or any affected areas will be wiped down and cleaned.</li>
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<li>Hazardous waste such as chloroform waste are collected in special waste bins in the lab that will be treated by the University's hazardous waste disposal contractor.</li>
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<li>All members are required to take the biosafety lecture held by the University. All members have had experience in lab work and have had online trainings on safe lab practices.</li>
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<h3> Your Lab </h3>
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<li>We hold weekly meetings with faculty and graduate student advisors such that experienced wet lab scientists can provide advice and feedback on safe lab practices. Moreover, all of our team members have had lab experience before so that we can reduce the risk of human error. We have also chosen a harmless lab strain of E. coli to prevent the risk of introduction or spreading of harmful organisms.</li>
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<li>Our parts might be maliciously misused to produce a toxic or harmful metabolite. Since our project involves an in vivo feedback loop system, the harmful or toxic substance will also be toxic to the host organism, counteracting the antibiotic selective pressure. </li>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_2.png|The inside of our lab!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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Image:Example2_Lab_7.png|We decorated this part of our lab
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Image:Example2_Lab_8.png|Whatever else you want
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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Revision as of 01:15, 18 October 2014

Visit the Safety Hub to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.

Our Lab

Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space!

Our lab is a WHO BSL1 Lab, a low risk lab. We use a range of equipment from open areas, to laminar flow hoods, as well as standard lab safety practices. Cells cultures are killed with bleach before disposal, and general solid waste is collected into biohazard bags and disposed of.

All team members recieved training on biosafety levels, requirements for laboratory practices and equipment, biohazard containment, recombinant DNA, bioethics, fire safety, and personal protective equipment. Our institution's safety training policies are available here.

At our institution, we are supervised by the University of Chicago Office of Environmental Health and Safety. They provide aform as a guide to safety here. We are also under US law, which provides other guidelines here.

All of the strains that we are using are in risk group 1, with the potential to cause irritation, or affect kidneys

Specific safety concerns and responses include:

  • E. coli MG1655 and DH5a, which belong to risk group 1 and may pose the following risks: irritation to skin, eyes, and respiratory tract, may affect kidneys. All plates and cultures are disinfected with bleach after use, and the lab benches are wiped down in 70% EtOH and then dH2O to prevent contamination.
  • Some toxic or harmful chemicals such as ethidium bromide, chloroform and bleach. Team members are required to wear rubber gloves at all times in labs, and lab coats when handling harmful chemicals.
  • Other flammable chemicals such as isopropyl ethanol, ethanol, DMSO will be handled with care and jf spilled, the liquids will be soaked up and the lab bench or any affected areas will be wiped down and cleaned.
  • Hazardous waste such as chloroform waste are collected in special waste bins in the lab that will be treated by the University's hazardous waste disposal contractor.
  • All members are required to take the biosafety lecture held by the University. All members have had experience in lab work and have had online trainings on safe lab practices.
  • We hold weekly meetings with faculty and graduate student advisors such that experienced wet lab scientists can provide advice and feedback on safe lab practices. Moreover, all of our team members have had lab experience before so that we can reduce the risk of human error. We have also chosen a harmless lab strain of E. coli to prevent the risk of introduction or spreading of harmful organisms.
  • Our parts might be maliciously misused to produce a toxic or harmful metabolite. Since our project involves an in vivo feedback loop system, the harmful or toxic substance will also be toxic to the host organism, counteracting the antibiotic selective pressure.

Retrieved from "http://2014.igem.org/Team:UChicago/Safety"