"
Team:Oxford/protocols/Nanodrop: Finding DNA Concentration
From 2014.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
<h1>Nanodrop: Finding DNA Concentration</h1><BR><BR> | <h1>Nanodrop: Finding DNA Concentration</h1><BR><BR> | ||
| - | + | ||
<!--this will number your steps 1,2,3,etc--> | <!--this will number your steps 1,2,3,etc--> | ||
<ol style="list-style-type:decimal;"> | <ol style="list-style-type:decimal;"> | ||
| Line 17: | Line 17: | ||
<li>Blot off the sample from the contacts as before and repeat for other samples. <BR><BR> | <li>Blot off the sample from the contacts as before and repeat for other samples. <BR><BR> | ||
<li>At the end of your use wash the contacts by adding 2µl water to the contacts and closing the lever a few times before blotting off the water to leave the machine dry. <BR><BR> | <li>At the end of your use wash the contacts by adding 2µl water to the contacts and closing the lever a few times before blotting off the water to leave the machine dry. <BR><BR> | ||
| - | |||
{{:Team:Oxford/templates/footer}} | {{:Team:Oxford/templates/footer}} | ||
Revision as of 11:23, 18 July 2014
Nanodrop: Finding DNA Concentration
- Ensure the program is selected to read “NUCLEIC ACID” where it says “Sample Type” (Top right of screen)
- Load 2 µl of your blank onto the raised pedestal. (NOTE: this blank should be the solvent your DNA/RNA is suspended in. For example this could be EB buffer if you have just done a MiniPrep)
- Lower the metal lever until it gently rests on the sample pedestal and click ‘BLANK’ to zero the spectrophotometer reading.
- Using a tissue blot off the liquid from the pedestal and the contact on the underside of the lever.
- Now load 2µl of your nucleic acid sample in the same manner as the blank and close the lever
- Click ‘MEASURE’. After a short while it will display a concentration in ng/µl in the bottom right hand corner.
- Also displayed is the UV/Vis spectrum. One should expect a clean ‘bell’-like curve that indicated a clean sample. If a rough and wavy spectrum is seen the sample is likely contaminated.
- Blot off the sample from the contacts as before and repeat for other samples.
- At the end of your use wash the contacts by adding 2µl water to the contacts and closing the lever a few times before blotting off the water to leave the machine dry.
