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Team:Sumbawagen/Notebook/protocol7
From 2014.igem.org
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<li>Plasmid DNA, 1.5 ul*</li> | <li>Plasmid DNA, 1.5 ul*</li> | ||
<li>Ligation kit (Takara), 5 ul</li> | <li>Ligation kit (Takara), 5 ul</li> | ||
| - | Total volume, 10 ul</li> | + | Total volume, 10 ul</li><br> |
| - | * final quantity insert DNA > plasmid DNA | + | * final quantity insert DNA > plasmid DNA </li> |
| - | </ | + | </ul> |
<p>2. Samples were incubated at 4 oC for overnight (inside a refrigerator).</p> | <p>2. Samples were incubated at 4 oC for overnight (inside a refrigerator).</p> | ||
Revision as of 01:07, 18 October 2014
Team:Sumbawagen/Team
From 2014.igem.org
Notebook – Protocol – 7. Ligation
1. Ligation reaction was as follow:
- Insert DNA, 2,5 ul*
- Plasmid DNA, 1.5 ul*
- Ligation kit (Takara), 5 ul Total volume, 10 ul
* final quantity insert DNA > plasmid DNA
2. Samples were incubated at 4 oC for overnight (inside a refrigerator).
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