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- | <h2>p14rz_002</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1</li>
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- | <li>Digest: pKM006, EcoRI + NotI; PCR-product</li>
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- | <li>Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/d/dc/2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- | </div>
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- | </section>
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- | <h2>p14rz_003</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 3</li>
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- | <li>Digest 3, 4</li>
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- | <li>Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
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- | <figcaption>
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- | <p class="header">Digest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <h2>p14rz_004</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 21,22</li>
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- | <li>Digest -</li>
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- | <li>Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <h2>p14rz_005</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 4,5</li>
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- | <li>Digest 5</li>
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- | <li>Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/5f/2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
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- | <figcaption>
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- | <p class="header">Digest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <h2>p14rz_006</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 17,18</li>
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- | <li>Digest 5</li>
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- | <li>Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/5e/2014Freiburg_140811_PCR1%2C17%2C18.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
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- | <figcaption>
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- | <p class="header">Digest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <h2>p14rz_007</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 37,38</li>
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- | <li>Digest 21</li>
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- | <li>Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
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- | <figcaption>
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- | <p class="header">Digest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/00/2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <h2>p14rz_008</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR blue light receptor; Template: pQXCIN-SLC7A1</li>
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- | <li>Digest pKM006 EcoRI, NotI</li>
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- | <li>Testdigest with SmaI in Cutsmart, expected bands: 5,7</li>
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- | </ul>
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- |
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- | </div>
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- | <div class="col-sm-6">
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- |
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/c8/2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- | </div>
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- | </div>
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- | </section>
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- | <h2>p14rz_009</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 39,40</li>
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- | <li>Digest 5</li>
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- | <li>Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- |
| |
- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
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- | <figcaption>
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- | <p class="header">Digest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | <div class="col-sm-6">
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- |
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
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- | <figcaption>
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- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <h2>p14rz_010</h2>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 23,24</li>
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- | <li>Digest 12</li>
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- | <li>Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56</li>
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- | </ul>
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- | </div>
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- | <div class="col-sm-6">
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- |
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- | <section>
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- | <div class="row category-row">
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- | <div class="col-sm-6">
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- |
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- | <figure>
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- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
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- | <figcaption>
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- | <p class="header">PCR</p>
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- | </figcaption>
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- | </figure>
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- |
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- |
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- | </div>
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- | <div class="col-sm-6">
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- |
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- |
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- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/c0/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
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- | </figcaption>
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- | </figure>
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- | </div>
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- | </div>
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- | </section>
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- |
| |
- | <h2>p14ls_003</h2>
| |
- | <section>
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- | <div class="row category-row">
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- |
| |
- | <div class="col-sm-6">
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- | <ul>
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- | <li>PCR 7,9,10</li>
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- | <li>Digest -</li>
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- | <li>Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4</li>
| |
- | </ul>
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- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/60/2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_001</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR Primers vv003,vv004; Template: pMIG</li>
| |
- | <li>Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI</li>
| |
- | <li>Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b7/2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_002</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR -</li>
| |
- | <li>Digest 8,11</li>
| |
- | <li>Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/09/2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/1/19/2014Freiburg_140822_Digest8%2C_11%2C_10.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/51/2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_003</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR pMIG with primer vv003+vv004-</li>
| |
- | <li>Digest xx</li>
| |
- | <li>Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/06/2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/1/1f/2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_006</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 26</li>
| |
- | <li>Digest 13</li>
| |
- | <li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/a/a1/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_007</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 27</li>
| |
- | <li>Digest 14</li>
| |
- | <li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cb/2014Freiburg_9_10_2014_2_13_33_PM_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_008</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 43</li>
| |
- | <li>Digest 22</li>
| |
- | <li>Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_009</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 44</li>
| |
- | <li>Digest 22</li>
| |
- | <li>Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_010</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 45</li>
| |
- | <li>Digest 22</li>
| |
- | <li>Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_012</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 46</li>
| |
- | <li>Digest 22</li>
| |
- | <li>Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_013</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 47,48,49</li>
| |
- | <li>Digest 22</li>
| |
- | <li>Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14vv_014</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 50,51</li>
| |
- | <li>Digest 22</li>
| |
- | <li>Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14zl_010</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 1,2</li>
| |
- | <li>Digest 1</li>
| |
- | <li>Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14zl_011</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 19,20</li>
| |
- | <li>Digest 1</li>
| |
- | <li>Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b0/2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14zl_016</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 52,53</li>
| |
- | <li>Digest 22</li>
| |
- | <li>Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14zl_017</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 64,65</li>
| |
- | <li>Digest 24</li>
| |
- | <li>testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR_digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b3/2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <h2>p14zl_020</h2>
| |
- | <section>
| |
- | <div class="row category-row">
| |
- |
| |
- | <div class="col-sm-6">
| |
- | <ul>
| |
- | <li>PCR 59,60</li>
| |
- | <li>Digest 24</li>
| |
- | <li>testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8</li>
| |
- | </ul>
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
| |
- | <figcaption>
| |
- | <p class="header">PCR</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
- |
| |
- | <section>
| |
- | <div class="row category-row">
| |
- | <div class="col-sm-6">
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Digest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | <div class="col-sm-6">
| |
- |
| |
- |
| |
- | <figure>
| |
- | <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
| |
- | <figcaption>
| |
- | <p class="header">Testdigest</p>
| |
- | </figcaption>
| |
- | </figure>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </section>
| |
| | | |
| | | |
| | | |
| + | <table width="897"> |
| + | <tbody> |
| + | <tr> |
| + | <td width="48">ID</td> |
| + | <td width="159">Template</td> |
| + | <td width="157">Primer 1</td> |
| + | <td width="79">Primer 2</td> |
| + | <td width="65">Size[kbp]</td> |
| + | <td width="389"> </td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 1</td> |
| + | <td>pKM292</td> |
| + | <td>o14zl_039</td> |
| + | <td>o14zl_040</td> |
| + | <td>1,3</td> |
| + | <td>p14zl_010_pLXV_Gal4BD_LOV_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 2</td> |
| + | <td>p14zl_003</td> |
| + | <td>o14zl_041</td> |
| + | <td>o14zl_042</td> |
| + | <td>0,66</td> |
| + | <td>p14zl_010_pLXV_Gal4BD_LOV_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 3</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14rz_016</td> |
| + | <td>o14rz_017</td> |
| + | <td>1,87</td> |
| + | <td>p14rz_003_pcDNA_SLC71A_GFP</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 4</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14rz_018</td> |
| + | <td>o14rz_019</td> |
| + | <td>1,87</td> |
| + | <td>p14rz_005_pcDNA_SLC7A1_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 5</td> |
| + | <td>pKM078</td> |
| + | <td>o14rz_020</td> |
| + | <td>o14rz_021</td> |
| + | <td>0,78</td> |
| + | <td>p14rz_005_pcDNA_SLC7A1_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 6</td> |
| + | <td>p14rz_002</td> |
| + | <td>o14rz_022</td> |
| + | <td>o14rz_023</td> |
| + | <td>1,93</td> |
| + | <td>p14rz_004_pcDNA_SLC7A1_HA</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 7</td> |
| + | <td>pKM084</td> |
| + | <td>o14_ls_013</td> |
| + | <td>o14_ls_014</td> |
| + | <td>3,9</td> |
| + | <td>p14ls_002_Gal4_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 8</td> |
| + | <td>pKM078</td> |
| + | <td>o14_ls_015</td> |
| + | <td>o14_ls_016</td> |
| + | <td>0,78</td> |
| + | <td>p14ls_002_Gal4_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 9</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14_ls_017</td> |
| + | <td>o14_ls_018</td> |
| + | <td>1,93</td> |
| + | <td>p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 10</td> |
| + | <td>pBK001</td> |
| + | <td>o14_ls_019</td> |
| + | <td>o14_ls_020</td> |
| + | <td>0,87</td> |
| + | <td>p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 11</td> |
| + | <td>pKM022</td> |
| + | <td>o14_ls_005</td> |
| + | <td>o14_ls_006</td> |
| + | <td>2,8</td> |
| + | <td>p14ls_001_COP1-VP16_TetR_UVR8</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 12</td> |
| + | <td>Digest 7</td> |
| + | <td>o14_ls_008</td> |
| + | <td>o14_ls_009</td> |
| + | <td>1,4</td> |
| + | <td>p14ls_001_COP1-VP16_TetR_UVR8</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 13</td> |
| + | <td>UVR8 (Bba_1150029)</td> |
| + | <td>o14_ls_010</td> |
| + | <td>o14_ls_011</td> |
| + | <td>1,2</td> |
| + | <td>p14ls_001_COP1-VP16_TetR_UVR8</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 14</td> |
| + | <td>psi_pack_synthese</td> |
| + | <td>o14vv_031</td> |
| + | <td>o14vv_032</td> |
| + | <td>0,64</td> |
| + | <td>pMIG_Standardisierung (Synthese rein)</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 15</td> |
| + | <td>p14vv_003_backbone</td> |
| + | <td>o14vv_033</td> |
| + | <td>o14vv_034</td> |
| + | <td>3,4</td> |
| + | <td>pMIG_Standardisierung (XbaI raus)</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 16</td> |
| + | <td>COP1_VP16(Patrick)</td> |
| + | <td>o14_ls_007</td> |
| + | <td>o14_ls_012</td> |
| + | <td>1,5</td> |
| + | <td>p14ls_001_COP1-VP16_TetR_UVR8</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 17</td> |
| + | <td>pBK001</td> |
| + | <td>o14_rz_028</td> |
| + | <td>o14_rz_029</td> |
| + | <td>0,84</td> |
| + | <td>p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 18</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14_rz_026</td> |
| + | <td>o14_rz_027</td> |
| + | <td>1,9</td> |
| + | <td>p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 19</td> |
| + | <td>pKM297</td> |
| + | <td>o14zl_035</td> |
| + | <td>o14zl_036</td> |
| + | <td>1,5</td> |
| + | <td>p14zl_011_pLXV_ePDZ_VP16_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 20</td> |
| + | <td>pKM078</td> |
| + | <td>o14zl_037</td> |
| + | <td>o14zl_043</td> |
| + | <td>0,8</td> |
| + | <td>p14zl_011_pLXV_ePDZ_VP16_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 21</td> |
| + | <td>pCDNA</td> |
| + | <td>o14rz_024</td> |
| + | <td>o14rz_025</td> |
| + | <td>5,4</td> |
| + | <td>p14rz_004_pcDNA_SLC7A1_HA_2</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 22</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14rz_030</td> |
| + | <td>o14rz_031</td> |
| + | <td>1,9</td> |
| + | <td>p14rz_004_pcDNA_SLC7A1_HA_2</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 23</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14rz_032</td> |
| + | <td>o14rz_033</td> |
| + | <td>1,9</td> |
| + | <td>p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 24</td> |
| + | <td>pBK001</td> |
| + | <td>o14rz_034</td> |
| + | <td>o14rz_035</td> |
| + | <td>0,85</td> |
| + | <td>p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 25</td> |
| + | <td>p14zl_003</td> |
| + | <td>014zl_010</td> |
| + | <td>014zl_011</td> |
| + | <td>5,4</td> |
| + | <td>p14zl_004_pMIG_Phyb_VP16_puro_TetR_PIF6</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 26</td> |
| + | <td>pKM006</td> |
| + | <td>o14vv_037</td> |
| + | <td>o14vv_038</td> |
| + | <td>1,6</td> |
| + | <td>p14vv_006_pMIG_CMV_SEAP</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 27</td> |
| + | <td>pKM006</td> |
| + | <td>o14vv_037</td> |
| + | <td>o14vv_039</td> |
| + | <td>1,6</td> |
| + | <td>p14vv_007_pMIG_SEAP</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 28</td> |
| + | <td>pLXV</td> |
| + | <td>o14zl_044_pLXV_rv_neu</td> |
| + | <td>o14zl_030</td> |
| + | <td>6,3</td> |
| + | <td>p14zl_008_pLXV_TetO13_CMVmin_SLC7A1</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 29</td> |
| + | <td>p14rz_006</td> |
| + | <td>o14zl_048</td> |
| + | <td>o14zl_049</td> |
| + | <td>2,7</td> |
| + | <td>p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 30</td> |
| + | <td>p14vv_003_backbone</td> |
| + | <td>o14zl_050</td> |
| + | <td>o14zl_051</td> |
| + | <td>4,9</td> |
| + | <td>p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 31</td> |
| + | <td>lenti-CRISPR</td> |
| + | <td>o14zl_020</td> |
| + | <td>o14zl_021</td> |
| + | <td>0,7</td> |
| + | <td>p14zl_007_pLXV_LOV_ePDZ_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 32</td> |
| + | <td>pKM297</td> |
| + | <td>o14zl_022</td> |
| + | <td>o14zl_023</td> |
| + | <td>1</td> |
| + | <td>p14zl_007_pLXV_LOV_ePDZ_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 33</td> |
| + | <td>pKM292</td> |
| + | <td>o14zl_024</td> |
| + | <td>o14zl_025</td> |
| + | <td>1,4</td> |
| + | <td>p14zl_007_pLXV_LOV_ePDZ_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 34</td> |
| + | <td>pKM022</td> |
| + | <td>o14zl_026</td> |
| + | <td>o14zl_027</td> |
| + | <td>0,6</td> |
| + | <td>p14zl_007_pLXV_LOV_ePDZ_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 35</td> |
| + | <td>p14rz_006</td> |
| + | <td>o14rz_033</td> |
| + | <td>o14rz_036</td> |
| + | <td>2</td> |
| + | <td>p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 36</td> |
| + | <td>pGEMHE-XfA4-mCherry</td> |
| + | <td>o14rz_035</td> |
| + | <td>o14rz_037</td> |
| + | <td>0,8</td> |
| + | <td>p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 37</td> |
| + | <td>p14rz_006</td> |
| + | <td>o14rz_026</td> |
| + | <td>o14rz_036</td> |
| + | <td>2</td> |
| + | <td>p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 38</td> |
| + | <td>pBK001</td> |
| + | <td>o14rz_029</td> |
| + | <td>o14rz_037</td> |
| + | <td>0,8</td> |
| + | <td>p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 39</td> |
| + | <td>pKM084</td> |
| + | <td>o14rz_040</td> |
| + | <td>o14rz_041</td> |
| + | <td>3,9</td> |
| + | <td>p14rz_009_Gal4_SLC7A1-HA</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 40</td> |
| + | <td>p14rz_002</td> |
| + | <td>o14rz_039</td> |
| + | <td>o14rz_038</td> |
| + | <td>2</td> |
| + | <td>p14rz_009_Gal4_SLC7A1-HA</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 41</td> |
| + | <td>pMIG</td> |
| + | <td>o14rz_042</td> |
| + | <td>o14rz_043</td> |
| + | <td>1,4</td> |
| + | <td>p14rz_012_pcDNA_SLC7A1_IRES_GFP</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 42</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14rz_044</td> |
| + | <td>o14rz_045</td> |
| + | <td>2</td> |
| + | <td>p14rz_012_pcDNA_SLC7A1_IRES_GFP</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 43</td> |
| + | <td>mTAG_BFP2</td> |
| + | <td>o14vv_044</td> |
| + | <td>o14vv_045</td> |
| + | <td>0,8</td> |
| + | <td>p14vv_008_pMIG_mTAG_BFP2</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 44</td> |
| + | <td>mKO</td> |
| + | <td>o14vv_042</td> |
| + | <td>o14vv_043</td> |
| + | <td>0,8</td> |
| + | <td>p14vv_009_pMIG_mKO</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 45</td> |
| + | <td>mKate</td> |
| + | <td>o14vv_046</td> |
| + | <td>o14vv_047</td> |
| + | <td>0,8</td> |
| + | <td>p14vv_010_pMIG_mKate</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 46</td> |
| + | <td>pQXIN SLC7A1</td> |
| + | <td>o14vv_050</td> |
| + | <td>o14vv_051</td> |
| + | <td>2</td> |
| + | <td>p14vv_012_pMIG_SLC7A1_IRES_GFP</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 47</td> |
| + | <td>p14zl_003</td> |
| + | <td>o14vv_054</td> |
| + | <td>o14vv_055</td> |
| + | <td>0,7</td> |
| + | <td>p14vv_013_pMIG_eGFP_IRES_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 48</td> |
| + | <td>p14zl_003</td> |
| + | <td>o14vv_052</td> |
| + | <td>o14vv_053</td> |
| + | <td>0,7</td> |
| + | <td>p14vv_013_pMIG_eGFP_IRES_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 49</td> |
| + | <td>pMIG</td> |
| + | <td>o14vv_056</td> |
| + | <td>o14vv_057</td> |
| + | <td>0,7</td> |
| + | <td>p14vv_013_pMIG_eGFP_IRES_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 50</td> |
| + | <td>pMIG</td> |
| + | <td>o14vv_056</td> |
| + | <td>o14vv_059</td> |
| + | <td>0,8</td> |
| + | <td>p14vv_014_pMIG_eGFP_p2a_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 51</td> |
| + | <td>p14zl_003</td> |
| + | <td>o14vv_055</td> |
| + | <td>o14vv_058</td> |
| + | <td>0,8</td> |
| + | <td>p14vv_014_pMIG_eGFP_p2a_puro</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 52</td> |
| + | <td>p14zl_003</td> |
| + | <td>o14zl_052</td> |
| + | <td>o14zl_053</td> |
| + | <td>2,4</td> |
| + | <td>p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 53</td> |
| + | <td>p14zl_003</td> |
| + | <td>o14zl_054</td> |
| + | <td>o14zl_055</td> |
| + | <td>2,4</td> |
| + | <td>p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 54</td> |
| + | <td>p14ls_001</td> |
| + | <td>o14zl_056</td> |
| + | <td>o14zl_057</td> |
| + | <td>4</td> |
| + | <td>p14zl_015_pMIG_COP1-VP16_TetR_UVR8</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 55</td> |
| + | <td>p14rz_008_Gal4_SLC7A1</td> |
| + | <td>p14zl_061</td> |
| + | <td>p14zl_062</td> |
| + | <td>0,27</td> |
| + | <td>p14zl_018_pLXV_Gal4_GFP_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 56</td> |
| + | <td>pEGFP-C1</td> |
| + | <td>p14zl_063</td> |
| + | <td>p14zl_064</td> |
| + | <td>0,83</td> |
| + | <td>p14zl_018_pLXV_Gal4_GFP_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 57</td> |
| + | <td>pMIG-venus</td> |
| + | <td>p14zl_058</td> |
| + | <td>p14zl_059</td> |
| + | <td>1,37</td> |
| + | <td>p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 58</td> |
| + | <td>p14rz_008_Gal4_SLC7A1</td> |
| + | <td>p14zl_061</td> |
| + | <td>p14zl_065</td> |
| + | <td>2,13</td> |
| + | <td>p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 59</td> |
| + | <td>pMIG-dsRED</td> |
| + | <td>p14zl_069</td> |
| + | <td>p14zl_068</td> |
| + | <td>1,34</td> |
| + | <td>p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 60</td> |
| + | <td>pKM292</td> |
| + | <td>p14zl_066</td> |
| + | <td>p14zl_067</td> |
| + | <td>1,4</td> |
| + | <td>p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 61</td> |
| + | <td>pMIG-Venus</td> |
| + | <td>p14zl_059</td> |
| + | <td>p14zl_076</td> |
| + | <td>0,61</td> |
| + | <td>p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 62</td> |
| + | <td>pGEMHE-XfA4-mKate</td> |
| + | <td>o14zl_074</td> |
| + | <td>p14zl_075</td> |
| + | <td>0,78</td> |
| + | <td>p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 63</td> |
| + | <td>p14rz_008</td> |
| + | <td>p14zl_061</td> |
| + | <td>p14zl_065</td> |
| + | <td>2,1</td> |
| + | <td>p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 64</td> |
| + | <td>pMIG-CFP</td> |
| + | <td>p14zl_059</td> |
| + | <td>p14zl_058</td> |
| + | <td>1,37</td> |
| + | <td>p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 65</td> |
| + | <td>pKM297</td> |
| + | <td>o14zl_035</td> |
| + | <td>p14zl_060</td> |
| + | <td>1,5</td> |
| + | <td>p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 66</td> |
| + | <td>p14rz_008_Gal4_SLC7A1</td> |
| + | <td>p14zl_070</td> |
| + | <td>p14zl_065</td> |
| + | <td>2,1</td> |
| + | <td>p14zl_022_pMIG_Gal4_SLC7A1_venus</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 67</td> |
| + | <td>pMIG-Venus</td> |
| + | <td>p14zl_059</td> |
| + | <td>p14zl_073</td> |
| + | <td>1,37</td> |
| + | <td>p14zl_022_pMIG_Gal4_SLC7A1_venus</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 68</td> |
| + | <td>p14rz_008_Gal4_SLC7A1</td> |
| + | <td>p14zl_070</td> |
| + | <td>p14zl_062</td> |
| + | <td>0,26</td> |
| + | <td>p14zl_023_pMIG_Gal4_GFP</td> |
| + | </tr> |
| + | <tr> |
| + | <td>PCR 69</td> |
| + | <td>pEGFP-C1</td> |
| + | <td>p14zl_072</td> |
| + | <td>p14zl_071</td> |
| + | <td>0,83</td> |
| + | <td>p14zl_023_pMIG_Gal4_GFP</td> |
| + | </tr> |
| + | </tbody> |
| + | </table> |
| + | <p> </p> |
| | | |
| </body> | | </body> |
| </html> | | </html> |