Team:Cambridge-JIC/Notebook/CL 2014 7 17

Salil
Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.

edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...

Trang-Anh
Planned constructs, things to order, wetwork. Listened to Gos's talk on lab technique. Tried to look at the Marchantia genome...

Salil
Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.

edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...

Ginny

1- checked restriction sites in UnaG- No Illegals hiding away!
2- equivocated most of the day whether to codn optimise not to codon optimise with each supervisor saying something different...conclusion: ummmmm
3- located source of 'cheap' bilirubin (unconjugated)
4- designed initial primers for UnaG constructs which are now obsolete as UnaG optimized sequence has changed... 4.bis: got confused with bioinforatics software with Angelina and Bernado- those 5' UTRs!!
5- made plans for tomorrow :P
(including catching up on the NewScientist and buying more more coffee- where is it all going?!)

Guy
I was in London for most of the day where by chance I met a farmer with all the knowledge and experience we could ever want about what would be useful to sense in agricultural soil. The outcome was that we'll be searching for phosphate inducible promoters, not nitrate ones, because nitrate tests are of less use given how easily washed away nitrates are. In the evening along with Salil I transformed and plated E. coli to amplify our chromoprotein genes, the transformant colonies should be visible in the morning.

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 (including catching up on the NewScientist and buying more more coffee- where is it all going?!)
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+<p><strong>Guy</strong><br>
+I was in London for most of the day where by chance I met a farmer with all the knowledge and experience we could ever want about what would be useful to sense in agricultural soil. The outcome was that we'll be searching for phosphate inducible promoters, not nitrate ones, because nitrate tests are of less use given how easily washed away nitrates are. In the evening along with Salil I transformed and plated E. coli to amplify our chromoprotein genes, the transformant colonies should be visible in the morning.
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