Team:Cambridge-JIC/Notebook/CL 2014 7 17

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<p><strong>Trang-Anh</strong><br>Planned constructs, things to order, wetwork. Listened to Gos's talk on lab technique. Tried to look at the Marchantia genome...</p>
<p><strong>Trang-Anh</strong><br>Planned constructs, things to order, wetwork. Listened to Gos's talk on lab technique. Tried to look at the Marchantia genome...</p>
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<p><strong>Salil</strong><br>Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.
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<br>
 +
<br>
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edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...
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</p>
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</p>
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<p><strong>Ginny</strong><br>
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<p>
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1- checked restriction sites in UnaG- No Illegals hiding away!
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<br>
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2- equivocated most of the day whether to codn optimise not to codon optimise with each supervisor saying something different...conclusion:  ummmmm
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<br>
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3- located source of 'cheap' bilirubin (unconjugated)
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<br>
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4- designed initial primers for UnaG constructs which are now obsolete as UnaG optimized sequence has changed...
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4.bis: got confused with bioinforatics software with Angelina and Bernado- those 5' UTRs!!
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<br>
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5- made plans for tomorrow :P
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<br>
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6- some other random stuff
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</p>
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Revision as of 08:54, 18 July 2014

Home Team Official Team Profile TimeLine Project Outreach Marchantia Informatics Parts Modeling Notebook Protocols Safety Dry Work Technology Attributions

Hugh
Work on the wiki; listened to Gos Micklem discuss good lab technique

Salil
Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.

edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...

Trang-Anh
Planned constructs, things to order, wetwork. Listened to Gos's talk on lab technique. Tried to look at the Marchantia genome...

Salil
Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.

edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...

Ginny

1- checked restriction sites in UnaG- No Illegals hiding away!
2- equivocated most of the day whether to codn optimise not to codon optimise with each supervisor saying something different...conclusion: ummmmm
3- located source of 'cheap' bilirubin (unconjugated)
4- designed initial primers for UnaG constructs which are now obsolete as UnaG optimized sequence has changed... 4.bis: got confused with bioinforatics software with Angelina and Bernado- those 5' UTRs!!
5- made plans for tomorrow :P
6- some other random stuff

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